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Article

Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma

  • Authors:
    • Yang Ling
    • Changsong Zhang
    • Yun Xu
    • Jing Zhu
    • Changtai Zhu
    • Mingzhu Lu
    • Yongping Liu
    • Tianbao Zhou
  • View Affiliations / Copyright

    Affiliations: Clinical Oncology Laboratory, Changzhou Tumor Hospital, Medical College of Soochow University, Changzhou, Jiangsu 213001, P.R. China, Department of Oncology, Nanyang Center Hospital, Nanyang, Henan 473000, P.R. China, The Hepatobiliary Surgery Center, The Affiliated Ningbo No. 2 Hospital, Ningbo University School of Medicine, Ningbo, Zhejiang 315010, P.R. China
  • Pages: 1075-1079
    |
    Published online on: January 8, 2014
       https://doi.org/10.3892/mmr.2014.1887
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Abstract

The aim of the present study was to determine the frequency of p27kip1 promoter methylation in esophageal squamous cell carcinoma (ESCC). The methylation status of the p27kip1 promoter was analyzed by methylation‑specific polymerase chain reaction (MSP) in 50 ESCC and matched non‑tumor tissues. Cell lines were treated with the demethylation agent 5‑aza‑2'‑deoxycytidine (5‑Aza‑CdR) and p27kip1 mRNA expression was detected by quantitative polymerase chain reaction. p27kip1 methylation was found in 36% (18/50) of ESCC patients, but only in 12% (6/50) of the corresponding non‑tumor tissues (P=0.005). There were statistically significant associations between the presence of methylation and tumor metastasis (P=0.002). The p27kip1 mRNA was lower in ESCC compared with non‑tumor tissues (mean ± standard deviation, ‑0.886±3.298 vs. 0.988±0.257; P=0.0033). Furthermore, a significant association was identified between the methylation status of the p27kip1 promoter and p27kip1 mRNA expression in the tissue (P<0.01). Thus, demethylation by 5‑Aza‑CdR was capable of inducing p27kip1 mRNA expression in esophageal cancer cell lines. The high promoter methylation of p27kip1 is a common phenomenon in ESCC, which may be an important mechanism of silencing p27kip1 mRNA expression.
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Copy and paste a formatted citation
Spandidos Publications style
Ling Y, Zhang C, Xu Y, Zhu J, Zhu C, Lu M, Liu Y and Zhou T: Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma. Mol Med Rep 9: 1075-1079, 2014.
APA
Ling, Y., Zhang, C., Xu, Y., Zhu, J., Zhu, C., Lu, M. ... Zhou, T. (2014). Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma. Molecular Medicine Reports, 9, 1075-1079. https://doi.org/10.3892/mmr.2014.1887
MLA
Ling, Y., Zhang, C., Xu, Y., Zhu, J., Zhu, C., Lu, M., Liu, Y., Zhou, T."Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma". Molecular Medicine Reports 9.3 (2014): 1075-1079.
Chicago
Ling, Y., Zhang, C., Xu, Y., Zhu, J., Zhu, C., Lu, M., Liu, Y., Zhou, T."Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma". Molecular Medicine Reports 9, no. 3 (2014): 1075-1079. https://doi.org/10.3892/mmr.2014.1887
Copy and paste a formatted citation
x
Spandidos Publications style
Ling Y, Zhang C, Xu Y, Zhu J, Zhu C, Lu M, Liu Y and Zhou T: Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma. Mol Med Rep 9: 1075-1079, 2014.
APA
Ling, Y., Zhang, C., Xu, Y., Zhu, J., Zhu, C., Lu, M. ... Zhou, T. (2014). Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma. Molecular Medicine Reports, 9, 1075-1079. https://doi.org/10.3892/mmr.2014.1887
MLA
Ling, Y., Zhang, C., Xu, Y., Zhu, J., Zhu, C., Lu, M., Liu, Y., Zhou, T."Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma". Molecular Medicine Reports 9.3 (2014): 1075-1079.
Chicago
Ling, Y., Zhang, C., Xu, Y., Zhu, J., Zhu, C., Lu, M., Liu, Y., Zhou, T."Promoter methylation‑associated silencing of p27kip1 gene with metastasis in esophageal squamous cell carcinoma". Molecular Medicine Reports 9, no. 3 (2014): 1075-1079. https://doi.org/10.3892/mmr.2014.1887
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