Facile synthesis and characterization of polyethylenimine-coated Fe3O4 superparamagnetic nanoparticles for cancer cell separation

Lu,Wei; Ling,Min; Jia,Min; Huang,Ping; Li,Chengkui; Yan,Biao

doi:10.3892/mmr.2014.1906

2014-March Volume 9 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

2014-March Volume 9 Issue 3

Full Size Image

Article

Facile synthesis and characterization of polyethylenimine-coated Fe3O4 superparamagnetic nanoparticles for cancer cell separation

Authors:
- Wei Lu
- Min Ling
- Min Jia
- Ping Huang
- Chengkui Li
- Biao Yan
View Affiliations / Copyright

Affiliations: School of Materials Science and Engineering, Shanghai Key Laboratory of D&A for Metal-Functional Materials, Tongji University, Shanghai 200092, P.R. China
Pages: 1080-1084
|
Published online on: January 20, 2014

https://doi.org/10.3892/mmr.2014.1906
Expand metrics +

Abstract

The detection of cancer cells in clinical samples is of great interest for a range of diagnostic applications, and separation and enrichment of cancer cells in low concentrations from complex sample matrices is necessary for efficient cancer diagnostics. In the present study, new surface-modified iron oxide nanoparticles were synthesized for the separation of lung cancer cells by simple precipitation of Fe(II) and Fe(III) salts in an aqueous ammonia solution, followed by the addition of polyethylenimine (PEI). The modified nanoparticles were characterized by X-ray diffractometry (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and vibrating sample magnetometry (VSM). XRD and TEM revealed that the particles were ~10 nm in diameter, while FTIR and XPS showed that their surfaces were well coated with PEI. VSM results confirmed the superparamagnetic nature of PEI-coated Fe3O4 nanoparticles. The separation and enrichment of lung cancer cells from sputum samples was demonstrated using the synthesized developed PEI-coated Fe3O4 magnetic nanoparticles. Exfoliative cytopathology showed that the percentage of positive cells increased from 6.3% (38/600) in untreated sputum samples to 38.5% (231/600) in sputum samples treated with PEI-coated Fe3O4 magnetic nanocomposites. This finding indicated that PEI-coated Fe3O4 magnetic nanocomposites can be used to efficiently enrich lung cancer cells from sputum for subsequent cytopathological analysis.

View Figures

View References

1	Babic M, Horák D, Trchová M, et al: Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labeling. Bioconjug Chem. 19:740–750. 2008. View Article : Google Scholar : PubMed/NCBI
2	Dong X, Zheng Y, Huang Y, et al: Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation. Anal Biochem. 405:207–212. 2010. View Article : Google Scholar : PubMed/NCBI
3	Imbeault M, Lodge R, Ouellet M and Tremblay MJ: Efficient magnetic bead-based separation of HIV-1-infected cells using an improved reporter virus system reveals that p53 up-regulation occurs exclusively in the virus-expressing cell population. Virology. 393:160–167. 2009. View Article : Google Scholar
4	Medina F, Segundo C, Salcedo I, et al: Purification of human lamina propria plasma cells by an immunomagnetic selection method. J Immunol Methods. 285:129–135. 2004. View Article : Google Scholar : PubMed/NCBI
5	Gupta AK and Gupta M: Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials. 26:3995–4021. 2005. View Article : Google Scholar : PubMed/NCBI
6	Abdallah B, Hassan A, Benoist C, et al: A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: polyethylenimine. Hum Gene Ther. 7:1947–1954. 1996. View Article : Google Scholar : PubMed/NCBI
7	Xu Y, Bruening ML and Watson JT: Use of polymer-modified MALDI-MS probes to improve analyses of protein digests and DNA. Anal Chem. 76:3106–3111. 2004. View Article : Google Scholar : PubMed/NCBI
8	Choosakoonkriang S, Lobo BA, Koe GS, et al: Biophysical characterization of PEI/DNA complexes. J Pharm Sci. 92:1710–1722. 2003. View Article : Google Scholar : PubMed/NCBI
9	Waldron RD: Infrared spectra of ferrites. Phys Rev. 99:1727–1735. 1955. View Article : Google Scholar
10	Banerjee I, Khollam YB, Balasubramanian C, et al: Preparation of γ-Fe₂O₃ nanoparticles using DC thermal arc-plasma route, their characterization and magnetic properties. Scr Mater. 54:1235–1240. 2006.
11	Fujii T, De Groot FMF, Sawatzky GA, et al: In situ XPS analysis of various iron oxide films grown by NO₂-assisted molecular-beam epitaxy. Phys Rev B. 59:3195–3202. 1999. View Article : Google Scholar
12	Qi HP, Chen QW, Wang MS, et al: Study of self-assembly of octahedral magnetite under an external magnetic field. J Phys Chem C. 113:17301–17305. 2009. View Article : Google Scholar
13	Asuha S, Zhou XG and Zhao S: Adsorption of methyl orange and Cr(VI) on mesoporous TiO₂ prepared by hydrothermal method. J Hazard Mater. 181:204–210. 2010. View Article : Google Scholar : PubMed/NCBI
14	Yamashita T and Hayes P: Analysis of XPS spectra of Fe²⁺ and Fe³⁺ ions in oxide materials. Appl Surf Sci. 254:2441–2449. 2008. View Article : Google Scholar
15	Graat PCJ and Somers AJ: Simultaneous determination of composition and thickness of thin iron-oxide films from XPS Fe 2p spectra. Appl Surf Sci. 100:36–40. 1996. View Article : Google Scholar
16	Cheng JP, Ma R, Chen X, et al: Effect of ferric ions on the morphology and size of magnetite nanocrystals synthesized by ultrasonic irradiation. Cryst Res Technol. 46:723–730. 2011. View Article : Google Scholar
17	Finn P and Jolly WL: Nitrogen 1s binding energies of transition metal nitrosyls. Inorg Chem. 11:893–895. 1972. View Article : Google Scholar
18	Incorvia MJ and Contarin S: X-ray photoelectron spectroscopic studies of metal/inhibitor systems: structure and bonding at the iron/amine interface. J Electrochem Soc. 136:2493–2498. 1989. View Article : Google Scholar
19	Berkowitz AE, Lahut JA and VanBuren CE: Properties of magnetic fluid particles. IEEE Trans Magn. 16:184–190. 1980. View Article : Google Scholar
20	Gnanaprakash G, Philip J, Jayakumar T, et al: Effect of digestion time and alkali addition rate on physical properties of magnetite nanoparticles. J Phys Chem B. 111:7978–7986. 2007. View Article : Google Scholar : PubMed/NCBI
21	Chantrell RW, Popplewell J and Charles SW: Measurements of particle size distribution parameters in ferrofluids. IEEE Trans Magn. 14:975–977. 1978. View Article : Google Scholar
22	Davies KJ, Wells S and Charles SW: The effect of temperature and oleate adsorption on the growth of maghemite particles. J Magn Magn Mater. 122:24–28. 1993. View Article : Google Scholar
23	Coey JMD: Noncollinear spin arrangement in ultrafine ferrimagnetic crystallites. Phys Rev Lett. 27:1140–1142. 1971. View Article : Google Scholar
24	Morup S: Mössbauer spectroscopy studies of suspensions of Fe₃O₄ microcrystals. J Magn Magn Mater. 39:45–47. 1983.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Facile synthesis and characterization of polyethylenimine-coated Fe3O4 superparamagnetic nanoparticles for cancer cell separation

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Related Articles