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Article

Screening for key genes associated with atopic dermatitis with DNA microarrays

  • Authors:
    • Zhong-Kui Zhang
    • Yong Yang
    • Shu-Rong Bai
    • Gui-Zhen Zhang
    • Tai‑Hua Liu
    • Zhou Zhou
    • Chun-Mei Wang
    • Li-Jun Tang
    • Jun Wang
    • Si-Xian He
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China, Department of Burns, Plastic Surgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China, Department of Anesthesiology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China, Department of Information, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China
  • Pages: 1049-1055
    |
    Published online on: January 21, 2014
       https://doi.org/10.3892/mmr.2014.1908
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Abstract

The aim of the present study was to identify key genes associated with atopic dermatitis (AD) using microarray data and bioinformatic analyses. The dataset GSE6012, downloaded from the Gene Expression Omnibus (GEO) database, contains gene expression data from 10 AD skin samples and 10 healthy skin samples. Following data preprocessing, differentially expressed genes (DEGs) were identified using the limma package of the R project. Interaction networks were constructed comprising DEGs that showed a degree of node of >3, >5 and >10, using the Osprey software. Functional enrichment and pathway enrichment analysis of the network comprising all DEGs and of the network comprising DEGs with a high degree of node, were performed with the DAVID and WebGestalt toolkits, respectively. A total of 337 DEGs were identified. The functional enrichment analysis revealed that the list of DEGs was significantly enriched for proteins related to epidermis development (P=2.95E-07), including loricrin (LOR), keratin 17 (KRT17), small proline-rich repeat proteins (SPRRs) and involucrin (IVL). The chemokine signaling pathway was the most significantly enriched pathway (P=0.0490978) in the network of all DEGs and in the network consisting of high degree‑node DEGs (>10), which comprised the genes coding for chemokine receptor 7 (CCR7), chemokine ligand (CCL19), signal transducer and activator of transcription 1 (STAT1), and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1). In conclusion, the list of AD-associated proteins identified in this study, including LOR, KRT17, SPRRs, IVL, CCR7, CCL19, PIK3R1 and STAT1 may prove useful for the development of methods to treat AD. From these proteins, PIK3R1 and KRT17 are novel and promising targets for AD therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Yang Y, Bai S, Zhang G, Liu TH, Zhou Z, Wang C, Tang L, Wang J, He S, He S, et al: Screening for key genes associated with atopic dermatitis with DNA microarrays. Mol Med Rep 9: 1049-1055, 2014.
APA
Zhang, Z., Yang, Y., Bai, S., Zhang, G., Liu, T., Zhou, Z. ... He, S. (2014). Screening for key genes associated with atopic dermatitis with DNA microarrays. Molecular Medicine Reports, 9, 1049-1055. https://doi.org/10.3892/mmr.2014.1908
MLA
Zhang, Z., Yang, Y., Bai, S., Zhang, G., Liu, T., Zhou, Z., Wang, C., Tang, L., Wang, J., He, S."Screening for key genes associated with atopic dermatitis with DNA microarrays". Molecular Medicine Reports 9.3 (2014): 1049-1055.
Chicago
Zhang, Z., Yang, Y., Bai, S., Zhang, G., Liu, T., Zhou, Z., Wang, C., Tang, L., Wang, J., He, S."Screening for key genes associated with atopic dermatitis with DNA microarrays". Molecular Medicine Reports 9, no. 3 (2014): 1049-1055. https://doi.org/10.3892/mmr.2014.1908
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Yang Y, Bai S, Zhang G, Liu TH, Zhou Z, Wang C, Tang L, Wang J, He S, He S, et al: Screening for key genes associated with atopic dermatitis with DNA microarrays. Mol Med Rep 9: 1049-1055, 2014.
APA
Zhang, Z., Yang, Y., Bai, S., Zhang, G., Liu, T., Zhou, Z. ... He, S. (2014). Screening for key genes associated with atopic dermatitis with DNA microarrays. Molecular Medicine Reports, 9, 1049-1055. https://doi.org/10.3892/mmr.2014.1908
MLA
Zhang, Z., Yang, Y., Bai, S., Zhang, G., Liu, T., Zhou, Z., Wang, C., Tang, L., Wang, J., He, S."Screening for key genes associated with atopic dermatitis with DNA microarrays". Molecular Medicine Reports 9.3 (2014): 1049-1055.
Chicago
Zhang, Z., Yang, Y., Bai, S., Zhang, G., Liu, T., Zhou, Z., Wang, C., Tang, L., Wang, J., He, S."Screening for key genes associated with atopic dermatitis with DNA microarrays". Molecular Medicine Reports 9, no. 3 (2014): 1049-1055. https://doi.org/10.3892/mmr.2014.1908
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