Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
2014-April Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-April Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats

  • Authors:
    • Jingxin Hong
    • Huajun Jin
    • Junling Han
    • Huanzhang Hu
    • Jian Liu
    • Linfang Li
    • Yao Huang
    • Dandan Wang
    • Mengchao Wu
    • Lugui Qiu
    • Qijun Qian
  • View Affiliations / Copyright

    Affiliations: Union Stem Cell and Gene Engineering Co., Ltd., Tianjin Cord Blood Bank, Tianjin 300384, P.R. China, Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Institute of Hematology, Blood Disease Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Tianjin 300020, P.R. China
  • Pages: 1103-1111
    |
    Published online on: January 30, 2014
       https://doi.org/10.3892/mmr.2014.1927
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cirrhosis is the long‑term outcome of chronic hepatic injury and no effective therapy is currently available for this disease. Mesenchymal stromal cells (MSCs) are multipotent cells that are easily acquired and amplified, and may be potential candidates for cell therapy against cirrhosis. This study aimed to determine the therapeutic effects of human umbilical cord‑derived MSCs (hUCMSCs) for the treatment of liver cirrhosis and identify an effective method for engrafting MSCs. The model of liver cirrhosis was established by induction of diethylnitrosamine (DEN) in rats. The isolated hUCMSCs were identified by morphology, flow cytometry and multilineage differentiation; they were injected into the vein of DEN‑induced rats at varied cell doses and infusion times. Biochemical analyses of the serum and histopathological analysis of the liver tissues were performed to evaluate the therapeutic effects of hUCMSCs in all treatment groups. The results indicated that isolated hUCMSCs were capable of self‑replication and differentiated into multiple lineages, including osteoblast‑, adipocyte‑ and hepatocyte‑like cells. Compared with the control group, administration of hUCMSCs at different cell doses and infusion times relieved DEN‑induced cirrhosis to varying degrees. The therapeutic effects of hUCMSCs on liver cirrhosis gradually improved with increased cell dose and infusion times. The improvement of cirrhosis was due to the capacity of hUCMSCs to breakdown collagen fibers in the liver. It was demonstrated that infusion of hUCMSCs effectively relieved liver cirrhosis by facilitating the breakdown of collagen fibers in a dose‑dependent manner and multiple infusions caused a relatively greater improvement in cirrhosis compared with a single infusion of hUCMSCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Friedman SL: Liver fibrosis - from bench to bedside. J Hepatol. 38(Suppl 1): S38–S53. 2003. View Article : Google Scholar

2 

Gines P, Quintero E, Arroyo V, Teres J, et al: Compensated cirrhosis: natural history and prognostic factors. Hepatology. 7:122–128. 1987. View Article : Google Scholar : PubMed/NCBI

3 

Moore KP and Aithal GP: Guidelines on the management of ascites in cirrhosis. Gut. 55(Suppl 6): vi1–vi12. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Sanyal AJ, Mullen KD and Bass NM: The treatment of hepatic encephalopathy in the cirrhotic patient. Gastroenterol Hepatol (NY). 6(Suppl 8): S1–S12. 2010.

5 

Murray KF and Carithers RL Jr; AASLD. AASLD practice guidelines: Evaluation of the patient for liver transplantation. Hepatology. 41:1407–1432. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Muraca M: Evolving concepts in cell therapy of liver disease and current clinical perspectives. Dig Liver Dis. 43:180–187. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Lee KD, Kuo TK, Whang-Peng J, et al: In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology. 40:1275–1284. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Chen Y, Dong XJ and Zhang GR: In vitro differentiation of mouse bone marrow stromal stem cells into hepatocytes induced by conditioned culture medium of hepatocytes. J Cell Biochem. 102:52–63. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Mohsin S, Shams S, Ali Nasir G, et al: Enhanced hepatic differentiation of mesenchymal stem cells after pretreatment with injured liver tissue. Differentiation. 81:42–48. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Sato Y, Araki H, Kato J, et al: Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion. Blood. 106:756–763. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Fang B, Shi M, Liao L, et al: Systemic infusions of FLK1(+) mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice. Transplantation. 78:83–88. 2004.

12 

Zhao DC, Lei JX, Chen R, et al: Bone marrow-derived mesenchymal stem cells protect against experimental liver fibrosis in rats. World J Gastroenterol. 11:3431–3440. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Abdel Aziz MT, Atta HM, Mahfouz S, et al: Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver fibrosis. Clin Biochem. 40:893–899. 2007.

14 

Chang YJ, Liu JW, Lin PC, et al: Mesenchymal stem cells facilitate recovery from chemically induced liver damage and decrease liver fibrosis. Life Sci. 85:517–525. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Lee MJ, Jung J, Na KH, et al: Anti-fibrotic effect of chorionic plate-derived mesenchymal stem cells isolated from human placenta in a rat model of CCl4-injured liver: potential application to the treatment of hepatic diseases. J Cell Biochem. 111:1453–1463. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Fan CG, Zhang QJ and Zhou JR: Therapeutic potentials of mesenchymal stem cells derived from human umbilical cord. Stem Cell Rev. 7:195–207. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Tsai PC, Fu TW, Chen YM, et al: The therapeutic potential of human umbilical mesenchymal stem cells from Wharton’s jelly in the treatment of rat liver fibrosis. Liver Transpl. 15:484–495. 2009.

18 

Wang HS, Hung SC, Peng ST, et al: Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord. Stem Cells. 22:1330–1337. 2004.

19 

Fisher LW, McBride OW, Termine JD, et al: Human bone sialoprotein. Deduced protein sequence and chromosomal localization. J Biol Chem. 265:2347–2351. 1990.PubMed/NCBI

20 

Avila RE, Carmo RA, de Farah KP, et al: Hyaluronic acid in the evaluation of liver fibrosis in patients with hepatitis C on haemodialysis. Braz J Infect Dis. 14:335–341. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Xu XM, Yuan GJ, Deng JJ, et al: Hepatic oval cells activated by hepatocyte apoptosis in diethylnitrosamine-induced rat liver cirrhosis. Saudi Med J. 31:490–494. 2010.PubMed/NCBI

22 

Barry FP and Murphy JM: Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol. 36:568–584. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Parekkadan B and Milwid JM: Mesenchymal stem cells as therapeutics. Annu Rev Biomed Eng. 12:87–117. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Thapa BR and Walia A: Liver function tests and their interpretation. Indian J Pediatr. 74:663–671. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Dai LJ, Li HY, Guan LX, et al: The therapeutic potential of bone marrow-derived mesenchymal stem cells on hepatic cirrhosis. Stem Cell Res. 2:16–25. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Chamberlain J, Yamagami T, Colletti E, et al: Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep. Hepatology. 46:1935–1945. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Parekkadan B, van Poll D, Suganuma K, et al: Mesenchymal stem cell-derived molecules reverse fulminant hepatic failure. PLoS One. 2:e9412007. View Article : Google Scholar : PubMed/NCBI

28 

van Poll D, Parekkadan B, Cho CH, et al: Mesenchymal stem cell-derived molecules directly modulate hepatocellular death and regeneration in vitro and in vivo. Hepatology. 47:1634–1643. 2008.PubMed/NCBI

29 

Chen X, Li Y, Wang L, Katakowski M, et al: Ischemic rat brain extracts induce human marrow stromal cell growth factor production. Neuropathology. 22:275–279. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hong J, Jin H, Han J, Hu H, Liu J, Li L, Huang Y, Wang D, Wu M, Qiu L, Qiu L, et al: Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats. Mol Med Rep 9: 1103-1111, 2014.
APA
Hong, J., Jin, H., Han, J., Hu, H., Liu, J., Li, L. ... Qian, Q. (2014). Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats. Molecular Medicine Reports, 9, 1103-1111. https://doi.org/10.3892/mmr.2014.1927
MLA
Hong, J., Jin, H., Han, J., Hu, H., Liu, J., Li, L., Huang, Y., Wang, D., Wu, M., Qiu, L., Qian, Q."Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats". Molecular Medicine Reports 9.4 (2014): 1103-1111.
Chicago
Hong, J., Jin, H., Han, J., Hu, H., Liu, J., Li, L., Huang, Y., Wang, D., Wu, M., Qiu, L., Qian, Q."Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats". Molecular Medicine Reports 9, no. 4 (2014): 1103-1111. https://doi.org/10.3892/mmr.2014.1927
Copy and paste a formatted citation
x
Spandidos Publications style
Hong J, Jin H, Han J, Hu H, Liu J, Li L, Huang Y, Wang D, Wu M, Qiu L, Qiu L, et al: Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats. Mol Med Rep 9: 1103-1111, 2014.
APA
Hong, J., Jin, H., Han, J., Hu, H., Liu, J., Li, L. ... Qian, Q. (2014). Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats. Molecular Medicine Reports, 9, 1103-1111. https://doi.org/10.3892/mmr.2014.1927
MLA
Hong, J., Jin, H., Han, J., Hu, H., Liu, J., Li, L., Huang, Y., Wang, D., Wu, M., Qiu, L., Qian, Q."Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats". Molecular Medicine Reports 9.4 (2014): 1103-1111.
Chicago
Hong, J., Jin, H., Han, J., Hu, H., Liu, J., Li, L., Huang, Y., Wang, D., Wu, M., Qiu, L., Qian, Q."Infusion of human umbilical cord‑derived mesenchymal stem cells effectively relieves liver cirrhosis in DEN‑induced rats". Molecular Medicine Reports 9, no. 4 (2014): 1103-1111. https://doi.org/10.3892/mmr.2014.1927
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team