Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
2014-April Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-April Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway

  • Authors:
    • Jing Liu
    • Guangbing Li
    • Dejie Liu
    • Jun Liu
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Liver Transplantation and Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Anesthesiology, Qilu Hospital Affiliated to Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 1289-1292
    |
    Published online on: January 30, 2014
       https://doi.org/10.3892/mmr.2014.1928
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hepatocellular carcinoma (HCC) is a primary cancer of the liver. Target therapy may improve prognosis of HCC. In the present study, we evaluated the inhibition of the Wnt/β‑catenin pathway as a potential therapeutic approach. HepG2 cells were treated with the β‑catenin inhibitor FH535. β‑catenin protein expression was semi‑quantitatively assessed using western blot analysis. Cell proliferation was examined with a 3‑(4,5‑dimethyl‑2‑yl)‑5‑(3‑carboxymethoxyphenyl)‑2‑(4‑sulfophenyl)‑2H‑tetrazolium salt (MTS) assay. The mRNA expression of nitric oxide synthase (iNOS) was detected by reverse transcription polymerase chain reaction. The Griess assay was used to determine nitric oxide (NO) concentration. FH535 inhibited the proliferation of HepG2 cells and decreased β‑catenin protein expression. mRNA expression of iNOS, a target gene of the Wnt/β‑catenin pathway, was decreased in FH535‑treated HepG2 cells compared to the control group. NO production was also reduced by FH535. In conclusion, the β‑catenin inhibitor FH535 may inhibit HCC cell proliferation via downregulation of the Wnt/β‑catenin pathway. Thus, targeting this pathway may be useful in HCC therapy.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

3 

Yang Z, Miao R, Li G, Wu Y, Robson SC, Yang X, Zhao Y, Zhao H and Zhong Y: Identification of recurrence related microRNAs in hepatocellular carcinoma after surgical resection. Int J Mol Sci. 14:1105–1118. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Bruix J and Sherman M: Management of hepatocellular carcinoma: an update. Hepatology. 53:1020–1022. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Wertheim JA, Petrowsky H, Saab S, Kupiec-Weglinski JW and Busuttil RW: Major challenges limiting liver transplantation in the United States. Am J Transplant. 11:1773–1784. 2011. View Article : Google Scholar : PubMed/NCBI

6 

El-Serag HB, Marrero JA, Rudolph L and Reddy KR: Diagnosis and treatment of hepatocellular carcinoma. Gastroenterology. 134:1752–1763. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Yeh JJ and Uemura M: Hepatocellular carcinoma. N Engl J Med. 366:92author reply 92–93. 2012. View Article : Google Scholar : PubMed/NCBI

8 

MacDonald BT, Tamai K and He X: Wnt/β-catenin signaling: components, mechanisms, and diseases. Dev Cell. 17:9–26. 2009.

9 

Thompson MD and Monga SP: WNT/β-catenin signaling in liver health and disease. Hepatology. 45:1298–1305. 2007.

10 

Clevers H: Wnt/β-catenin signaling in development and disease. Cell. 127:469–480. 2006.

11 

Burke ZD and Tosh D: The Wnt/β-catenin pathway: master regulator of liver zonation? Bioessays. 28:1072–1077. 2006.

12 

Siuta M, Zuckerman SL and Mocco J: Nitric oxide in cerebral vasospasm: theories, measurement, and treatment. Neurol Res Int. 2013:9724172013. View Article : Google Scholar : PubMed/NCBI

13 

Ozel RE, Alkasir RS, Ray K, Wallace KN and Andreescu S: Comparative evaluation of intestinal nitric oxide in embryonic zebrafish exposed to metal oxide nanoparticles. Small. 9:4250–4261. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Du Q, Zhang X, Cardinal J, Cao Z, Guo Z, Shao L and Geller DA: Wnt/beta-catenin signaling regulates cytokine-induced human inducible nitric oxide synthase expression by inhibiting nuclear factor-kappaB activation in cancer cells. Cancer Res. 69:3764–3771. 2009. View Article : Google Scholar

15 

Bosch FX, Ribes J, Diaz M and Cléries R: Primary liver cancer: worldwide incidence and trends. Gastroenterology. 127(Suppl 1): S5–S16. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Hora C, Romanque P and Dufour JF: Effect of sorafenib on murine liver regeneration. Hepatology. 53:577–586. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Pitot HC: Adventures in hepatocarcinogenesis. Annu Rev Pathol. 2:1–29. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Hussain SP, Schwank J, Staib F, Wang XW and Harris CC: TP53 mutations and hepatocellular carcinoma: insights into the etiology and pathogenesis of liver cancer. Oncogene. 26:2166–2176. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Willert K and Jones KA: Wnt signaling: is the party in the nucleus? Genes Dev. 20:1394–1404. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Gordon MD and Nusse R: Wnt signaling: multiple pathways, multiple receptors, and multiple transcription factors. J Biol Chem. 281:22429–22433. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Thu KL, Radulovich N, Becker-Santos DD, Pikor LA, Pusic A, Lockwood WW, Lam WL and Tsao MS: SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling. Oncogene. Jan 14–2013.(Epub ahead of print). View Article : Google Scholar

22 

Clements WM, Wang J, Sarnaik A, Kim OJ, MacDonald J, Fenoglio-Preiser C, Groden J and Lowy AM: β-Catenin mutation is a frequent cause of Wnt pathway activation in gastric cancer. Cancer Res. 62:3503–3506. 2002.

23 

Li H, Mo J, Jia G, Liu C, Luan Z and Guan Y: Activation of Wnt signaling inhibits the pro-apoptotic role of Notch in gastric cancer cells. Mol Med Rep. 7:1751–1756. 2013.PubMed/NCBI

24 

Shapiro M, Akiri G, Chin C, Wisnivesky JP, Beasley MB, Weiser TS, Swanson SJ and Aaronson SA: Wnt pathway activation predicts increased risk of tumor recurrence in patients with stage I nonsmall cell lung cancer. Ann Surg. 257:548–554. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Lustig B and Behrens J: The Wnt signaling pathway and its role in tumor development. J Cancer Res Clin Oncol. 129:199–221. 2003.PubMed/NCBI

26 

Luo X, Li HX, Liu RX, Wu ZS, Yang YJ and Yang GS: β-catenin protein utilized by Tumour necrosis factor-alpha in porcine preadipocytes to suppress differentiation. BMB Rep. 42:338–343. 2009.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu J, Li G, Liu D and Liu J: FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway. Mol Med Rep 9: 1289-1292, 2014.
APA
Liu, J., Li, G., Liu, D., & Liu, J. (2014). FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway. Molecular Medicine Reports, 9, 1289-1292. https://doi.org/10.3892/mmr.2014.1928
MLA
Liu, J., Li, G., Liu, D., Liu, J."FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 9.4 (2014): 1289-1292.
Chicago
Liu, J., Li, G., Liu, D., Liu, J."FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 9, no. 4 (2014): 1289-1292. https://doi.org/10.3892/mmr.2014.1928
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Li G, Liu D and Liu J: FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway. Mol Med Rep 9: 1289-1292, 2014.
APA
Liu, J., Li, G., Liu, D., & Liu, J. (2014). FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway. Molecular Medicine Reports, 9, 1289-1292. https://doi.org/10.3892/mmr.2014.1928
MLA
Liu, J., Li, G., Liu, D., Liu, J."FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 9.4 (2014): 1289-1292.
Chicago
Liu, J., Li, G., Liu, D., Liu, J."FH535 inhibits the proliferation of HepG2 cells via downregulation of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 9, no. 4 (2014): 1289-1292. https://doi.org/10.3892/mmr.2014.1928
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team