Naloxone induces endoplasmic reticulum stress in PC12 cells

  • Authors:
    • Soyoung Seo
    • Young-Sook Kwon
    • Kweon Yu
    • Seung-Whan Kim
    • O-Yu Kwon
    • Kyung-Hee Kang
    • Kisang Kwon
  • View Affiliations

  • Published online on: February 7, 2014     https://doi.org/10.3892/mmr.2014.1935
  • Pages: 1395-1399
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Abstract

Naloxone is an opioid inverse agonist used in the treatment of opiate overdose, with well known pharmacology. In the present study, we determined the effects of naloxone on the unfolded protein response (UPR) in PC12 cells. Data from a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that naloxone may accelerate PC12 cell apoptosis in a dose-dependent manner. We also demonstrated that naloxone upregulated gene expression of endoplasmic reticulum (ER) chaperones, including binding immunoglobulin protein (Bip), calnexin, ER protein 29 (ERp29) and protein disulfide isomerase (PDI), and ER stress sensors, including activating transcription factor 6 (ATF6), inositol-requiring enzyme 1 (IRE1) and protein kinase-like ER kinase (PERK). In addition, naloxone also induced typical ER stress phenomena, including ART6 proteolytic cleavage, eIF2α phosphorylation and XBP1 mRNA splicing. Furthermore, naloxone upregulated gene expression of ER chaperones and ER stress sensors in in vivo experiments. To the best of our knowledge, these results are the first to indicate that naloxone induces ER stress in vitro and in vivo.
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2014-April
Volume 9 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Seo S, Kwon Y, Yu K, Kim S, Kwon O, Kang K and Kwon K: Naloxone induces endoplasmic reticulum stress in PC12 cells. Mol Med Rep 9: 1395-1399, 2014.
APA
Seo, S., Kwon, Y., Yu, K., Kim, S., Kwon, O., Kang, K., & Kwon, K. (2014). Naloxone induces endoplasmic reticulum stress in PC12 cells. Molecular Medicine Reports, 9, 1395-1399. https://doi.org/10.3892/mmr.2014.1935
MLA
Seo, S., Kwon, Y., Yu, K., Kim, S., Kwon, O., Kang, K., Kwon, K."Naloxone induces endoplasmic reticulum stress in PC12 cells". Molecular Medicine Reports 9.4 (2014): 1395-1399.
Chicago
Seo, S., Kwon, Y., Yu, K., Kim, S., Kwon, O., Kang, K., Kwon, K."Naloxone induces endoplasmic reticulum stress in PC12 cells". Molecular Medicine Reports 9, no. 4 (2014): 1395-1399. https://doi.org/10.3892/mmr.2014.1935