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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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2014-April Volume 9 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Article

Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis

  • Authors:
    • Qiang Fan
    • Yuping Hu
    • Haidong Pang
    • Jintang Sun
    • Zhendong Wang
    • Jianmin Li
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Orthopedics, People's Hospital of Jimo, Qingdao, Shandong 266200, P.R. China
  • Pages: 1365-1370
    |
    Published online on: February 7, 2014
       https://doi.org/10.3892/mmr.2014.1936
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Abstract

The aim of the present study was to explore the pro‑apoptotic effect and specific mechanism of action of melittin (MEL) in humans. The effects of MEL on apoptosis in osteosarcoma and fetal osteoblast cells were investigated, and the mechanism that induced MG63 cell growth was also explored. The effects of MEL on cell proliferation were detected by a 2,3‑bis‑(2‑methoxy‑4‑nitro‑5‑sulfophenyl)-2H‑tetrazolium‑5‑carboxanilide analysis. Apoptosis was detected by flow cytometric analysis. MEL protein, inositol‑requiring protein‑1 (IRE‑α), phosphorylated‑protein kinase R‑like endoplasmic reticulum (ER) kinase, spliced X‑box‑1 (XBP1), eukaryotic translation initiation factor‑2α, cleaved activating transcription factor‑6, caspase‑12 and C/EBP homology protein (CHOP) were detected in three groups and two cell lines by western blot analysis. The results indicated that the expression or incubation of MEL in the MG63 cells triggered apoptosis and the inhibition of proliferation. One protein from the ER stress unfolded protein response pathway, IRE‑α, was involved in the MEL‑induced apoptosis in MG63 cells. Furthermore, spliced XBP1 protein was significantly increased in the MEL peptide incubated and MEL expressing groups of MG63 cells. Furthermore, CHOP protein expression was activated in MG63 cells following being incubated with or expressing MEL. In conclusion, MEL serves as an effective factor that inhibits the proliferation of MG63 cells via activating the ER stress‑mediated apoptosis pathway. This activation is triggered by the IRE‑α pathway mediated by inducing CHOP protein expression.
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Copy and paste a formatted citation
Spandidos Publications style
Fan Q, Hu Y, Pang H, Sun J, Wang Z and Li J: Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis . Mol Med Rep 9: 1365-1370, 2014.
APA
Fan, Q., Hu, Y., Pang, H., Sun, J., Wang, Z., & Li, J. (2014). Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis . Molecular Medicine Reports, 9, 1365-1370. https://doi.org/10.3892/mmr.2014.1936
MLA
Fan, Q., Hu, Y., Pang, H., Sun, J., Wang, Z., Li, J."Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis ". Molecular Medicine Reports 9.4 (2014): 1365-1370.
Chicago
Fan, Q., Hu, Y., Pang, H., Sun, J., Wang, Z., Li, J."Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis ". Molecular Medicine Reports 9, no. 4 (2014): 1365-1370. https://doi.org/10.3892/mmr.2014.1936
Copy and paste a formatted citation
x
Spandidos Publications style
Fan Q, Hu Y, Pang H, Sun J, Wang Z and Li J: Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis . Mol Med Rep 9: 1365-1370, 2014.
APA
Fan, Q., Hu, Y., Pang, H., Sun, J., Wang, Z., & Li, J. (2014). Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis . Molecular Medicine Reports, 9, 1365-1370. https://doi.org/10.3892/mmr.2014.1936
MLA
Fan, Q., Hu, Y., Pang, H., Sun, J., Wang, Z., Li, J."Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis ". Molecular Medicine Reports 9.4 (2014): 1365-1370.
Chicago
Fan, Q., Hu, Y., Pang, H., Sun, J., Wang, Z., Li, J."Melittin protein inhibits the proliferation of MG63 cells by activating inositol‑requiring protein‑1α and X‑box binding protein 1‑mediated apoptosis ". Molecular Medicine Reports 9, no. 4 (2014): 1365-1370. https://doi.org/10.3892/mmr.2014.1936
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