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Article

Incidence of bcr‑abl fusion transcripts in healthy individuals

  • Authors:
    • Said I. Ismail
    • Randa G. Naffa
    • Al‑Motassem F. Yousef
    • Majd T. Ghanim
  • View Affiliations / Copyright

    Affiliations: Molecular Biology Research Laboratory, Department of Biochemistry, Faculty of Medicine, University of Jordan, Amman 11942, Jordan, Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, University of Jordan, Amman 11942, Jordan
  • Pages: 1271-1276
    |
    Published online on: February 13, 2014
       https://doi.org/10.3892/mmr.2014.1951
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Abstract

Bcr‑abl fusion transcripts, resulting from translocation t(9;22), are hallmarks of Philadelphia chromosome positive (Ph+) leukemias. This translocation is detected in >90% of patients with chronic myelogenous leukemia and ~20% of acute lymphoblastic leukemia patients, which predominantly express the p210 and p190 proteins, respectively. Although the occurrence of t(9;22) in healthy individuals has been previously demonstrated, the number of studies is limited and the results are inconsistent. The present study screened for the presence of bcr‑abl transcripts in the blood of a group of healthy individuals using a sensitive‑nested reverse transcription polymerase chain reaction (RT‑PCR) assay. Samples were collected from 189 healthy volunteers (145 adults and 44 children). RNA was reverse transcribed and amplified by two rounds of PCR, amplifying the two common variants of bcr‑abl transcripts, p190 and p210. While the bcr‑abl p190 transcript was not detected, the p210 transcript was detected in ~10% of samples. Notably, the incidence of p210 translocation was higher in males (12.2%) compared with females (7.7%) and males were 2.4 times more likely to have the translocation. A significant incidence was also observed in adults compared with children, where adults were 6 times more likely to have the translocation. The presence of bcr‑abl transcripts in the blood of a significant proportion of healthy individuals should be considered in long‑term investigations to establish its exact association with the risk of developing leukemia. Furthermore, the current assays should be revised to consider the proportion of normal samples carrying the p210 transcripts when making a differential diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Ismail SI, Naffa RG, Yousef AMF and Ghanim MT: Incidence of bcr‑abl fusion transcripts in healthy individuals. Mol Med Rep 9: 1271-1276, 2014.
APA
Ismail, S.I., Naffa, R.G., Yousef, A.F., & Ghanim, M.T. (2014). Incidence of bcr‑abl fusion transcripts in healthy individuals. Molecular Medicine Reports, 9, 1271-1276. https://doi.org/10.3892/mmr.2014.1951
MLA
Ismail, S. I., Naffa, R. G., Yousef, A. F., Ghanim, M. T."Incidence of bcr‑abl fusion transcripts in healthy individuals". Molecular Medicine Reports 9.4 (2014): 1271-1276.
Chicago
Ismail, S. I., Naffa, R. G., Yousef, A. F., Ghanim, M. T."Incidence of bcr‑abl fusion transcripts in healthy individuals". Molecular Medicine Reports 9, no. 4 (2014): 1271-1276. https://doi.org/10.3892/mmr.2014.1951
Copy and paste a formatted citation
x
Spandidos Publications style
Ismail SI, Naffa RG, Yousef AMF and Ghanim MT: Incidence of bcr‑abl fusion transcripts in healthy individuals. Mol Med Rep 9: 1271-1276, 2014.
APA
Ismail, S.I., Naffa, R.G., Yousef, A.F., & Ghanim, M.T. (2014). Incidence of bcr‑abl fusion transcripts in healthy individuals. Molecular Medicine Reports, 9, 1271-1276. https://doi.org/10.3892/mmr.2014.1951
MLA
Ismail, S. I., Naffa, R. G., Yousef, A. F., Ghanim, M. T."Incidence of bcr‑abl fusion transcripts in healthy individuals". Molecular Medicine Reports 9.4 (2014): 1271-1276.
Chicago
Ismail, S. I., Naffa, R. G., Yousef, A. F., Ghanim, M. T."Incidence of bcr‑abl fusion transcripts in healthy individuals". Molecular Medicine Reports 9, no. 4 (2014): 1271-1276. https://doi.org/10.3892/mmr.2014.1951
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