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Article

Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface

  • Authors:
    • Jun-Beom Park
    • Gil Lee
    • Byeong Gon Yun
    • Chang-Hyen Kim
    • Youngkyung Ko
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea, Department of Dentistry, Graduate School, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea, Department of Oral and Maxillofacial Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
  • Pages: 1249-1253
    |
    Published online on: February 20, 2014
       https://doi.org/10.3892/mmr.2014.1971
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Abstract

Chlorhexidine (CHX) and Listerine (LIS), an essential oil compound, are the two commonly used adjunctive agents for mechanical debridement, for reducing the bacterial load in the treatment of peri-implant inflammation. However, antimicrobial agents have been reported to be cytotoxic to the alveolar bone cells and gingival epithelial cells. The present study was performed to examine the effects of antiseptics CHX and LIS, on the morphology and proliferation of stem cells. Stem cells derived from the buccal fat pad were grown on machined titanium discs. Each disc was immersed in CHX or LIS for 30 sec, 1.5 min or 4.5 min. Cell morphology was evaluated with a confocal laser microscope and the viability of the cells was quantitatively analyzed with the cell counting kit-8 (CCK-8). The untreated cells attached to the titanium discs demonstrated well-organized actin cytoskeletons. No marked alterations in the cytoskeletal organization were observed in any of the treated groups. The treatment with CHX and LIS of the titanium discs decreased the viability of the cells grown on the treated discs (P<0.05). The stem cells derived from the buccal fat pad were sensitive to CHX and LIS, and a reduction in cellular viability was observed when these agents were applied to the discs for 30 sec. Further studies are required to determine the optimal application time and concentration of this antimicrobial agent for maximizing the reduction of the bacterial load and minimizing the cytotoxicity to the surrounding cells.
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Copy and paste a formatted citation
Spandidos Publications style
Park J, Lee G, Yun BG, Kim C and Ko Y: Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface. Mol Med Rep 9: 1249-1253, 2014.
APA
Park, J., Lee, G., Yun, B.G., Kim, C., & Ko, Y. (2014). Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface. Molecular Medicine Reports, 9, 1249-1253. https://doi.org/10.3892/mmr.2014.1971
MLA
Park, J., Lee, G., Yun, B. G., Kim, C., Ko, Y."Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface". Molecular Medicine Reports 9.4 (2014): 1249-1253.
Chicago
Park, J., Lee, G., Yun, B. G., Kim, C., Ko, Y."Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface". Molecular Medicine Reports 9, no. 4 (2014): 1249-1253. https://doi.org/10.3892/mmr.2014.1971
Copy and paste a formatted citation
x
Spandidos Publications style
Park J, Lee G, Yun BG, Kim C and Ko Y: Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface. Mol Med Rep 9: 1249-1253, 2014.
APA
Park, J., Lee, G., Yun, B.G., Kim, C., & Ko, Y. (2014). Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface. Molecular Medicine Reports, 9, 1249-1253. https://doi.org/10.3892/mmr.2014.1971
MLA
Park, J., Lee, G., Yun, B. G., Kim, C., Ko, Y."Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface". Molecular Medicine Reports 9.4 (2014): 1249-1253.
Chicago
Park, J., Lee, G., Yun, B. G., Kim, C., Ko, Y."Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface". Molecular Medicine Reports 9, no. 4 (2014): 1249-1253. https://doi.org/10.3892/mmr.2014.1971
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