HPV16E7 silencing enhances susceptibility of CaSki cells to natural killer cells

Guo,Huimin; Hu,Ruili; Guan,Xinlei; Guo,Fang; Zhao,Shuzhen; Zhang,Xueying

doi:10.3892/mmr.2014.1975

2014-April Volume 9 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

2014-April Volume 9 Issue 4

Full Size Image

Article

HPV16E7 silencing enhances susceptibility of CaSki cells to natural killer cells

Authors:
- Huimin Guo
- Ruili Hu
- Xinlei Guan
- Fang Guo
- Shuzhen Zhao
- Xueying Zhang
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
Pages: 1351-1354
|
Published online on: February 20, 2014

https://doi.org/10.3892/mmr.2014.1975
Expand metrics +

Abstract

The aim of the present study was to investigate the cytotoxicity of natural killer (NK) cells to CaSki cells following knockdown of the E7 protein of the human papillomavirus type 16 (HPV16E7). Recombinant adenovirus-short hairpin-E7 protein of the human panillomavirus type 16 (Ad‑sh‑HPV16E7) was constructed and used to infect CaSki cells. The expression of HPV16E7 in CaSki cells was assessed using western blot analysis. The expression of cell surface molecule major histocompatibility complex‑I (MHC‑I) in CaSki cells infected with Ad‑sh‑HPV16E7 was examined using flow cytometry. The cytotoxicity of NK cells isolated and expanded from healthy volunteers on Ad‑sh‑HPV16E7‑infected CaSki cells was assessed using the lactate dehydrogenase (LDH) release assay. Ad‑sh‑HPV16E7 was successfully constructed and able to inhibit HPV16E7 the expression in CaSki cells. The expression of major histocompatibility complex I (MHC‑I), a surface molecule, in CaSki cells was increased after infection with Ad‑sh‑HPV16E7. Compared with the controls, the cytotoxicity of NK cells on CaSki cells, which were infected with Ad‑sh‑HPV16E7, was decreased (p<0.05). In conclusion, HPV16E7 suppresses the expression of MHC‑I on CaSki cells to evade cytotoxic T‑cell (CTL) response. However, it was possible to enhance the cytotoxicity of expanded NK cells to cervical cancer cells or HPV16‑infected cells in vitro, indicating that NK cells may be used for immunotherapy of cervical cancer.

View Figures

View References

1	Vaccarella S, Lortet-Tieulent J, Plummer M, Franceschi S and Bray F: Worldwide trends in cervical cancer incidence: Impact of screening against changes in disease risk factors. Eur J Cancer. 49:3262–3273. 2013. View Article : Google Scholar : PubMed/NCBI
2	Cheng M, Chen Y, Xiao W, Sun R and Tian Z: NK cell-based immunotherapy for malignant diseases. Cell Mol Immunol. 10:230–252. 2013. View Article : Google Scholar : PubMed/NCBI
3	Jost S and Altfeld M: Control of human viral infections by natural killer cells. Annu Rev Immunol. 31:163–194. 2013. View Article : Google Scholar : PubMed/NCBI
4	Cooper MA, Colonna M and Yokoyama WM: Hidden talents of natural killers: NK cells in innate and adaptive immunity. EMBO Rep. 10:1103–1110. 2009. View Article : Google Scholar : PubMed/NCBI
5	Bottley G, Cook GP and Blair GE: A flow cytometric assay for analysis of natural-killer cell-mediated cytolysis of adenovirus transformed cells. Methods Mol Med. 131:221–230. 2007. View Article : Google Scholar : PubMed/NCBI
6	Lim O, Lee Y, Chung H, et al: GMP-compliant, large-scale expanded allogeneic natural killer cells have potent cytolytic activity against cancer cells in vitro and in vivo. PLoS one. 8:e536112013. View Article : Google Scholar : PubMed/NCBI
7	Denman CJ, Senyukov VV, Somanchi SS, et al: Membrane-bound IL-21 promotes sustained ex vivo proliferation of human natural killer cells. PLoS one. 7:e302642012. View Article : Google Scholar : PubMed/NCBI
8	Shook DR and Campana D: Natural killer cell engineering for cellular therapy of cancer. Tissue Antigens. 78:409–415. 2011. View Article : Google Scholar : PubMed/NCBI
9	Zhao B and Ricciardi RP: E1A is the component of the MHC class I enhancer complex that mediates HDAC chromatin repression in adenovirus-12 tumorigenic cells. Virology. 352:338–344. 2006. View Article : Google Scholar : PubMed/NCBI
10	Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M and Geraghty DE: HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci USA. 95:5199–5204. 1998. View Article : Google Scholar : PubMed/NCBI
11	Kren L, Valkovsky I, Dolezel J, et al: HLA-G and HLA-E specific mRNAs connote opposite prognostic significance in renal cell carcinoma. Diagn Pathol. 7:582012. View Article : Google Scholar : PubMed/NCBI
12	Kren L, Fabian P, Slaby O, et al: Multifunctional immune-modulatory protein HLA-E identified in classical Hodgkin lymphoma: possible implications. Pathol Res Pract. 208:45–49. 2012. View Article : Google Scholar : PubMed/NCBI
13	McCoy WH IV, Wang X, Yokoyama WM, Hansen TH and Fremont DH: Cowpox virus employs a two-pronged strategy to outflank MHCI antigen presentation. Mol Immunol. 55:156–158. 2013. View Article : Google Scholar : PubMed/NCBI
14	Qiu T, Wang L, Liu XH, et al: Over-expressing transporters associated with antigen processing increases antitumor immunity response in prostate cancer. Cell Immunol. 279:167–173. 2012. View Article : Google Scholar : PubMed/NCBI
15	Oliveira CC and van Hall T: Importance of TAP-independent processing pathways. Mol Immunol. 55:113–116. 2013. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

HPV16E7 silencing enhances susceptibility of CaSki cells to natural killer cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles