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Article

c-Jun transactivates Puma gene expression to promote osteoarthritis

  • Authors:
    • Huading Lu
    • Gang Hou
    • Yongkai Zhang
    • Yuhu Dai
    • Huiqing Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, P.R. China
  • Pages: 1606-1612
    |
    Published online on: February 24, 2014
       https://doi.org/10.3892/mmr.2014.1981
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Abstract

Osteoarthritis (OA) is a chronic degenerative joint disorder in which genetic, hormonal, mechanical and ageing factors affect its progression. Current studies are focusing on chondrocytes as a key mediator of OA at a cellular level. however, the mechanism underlying chondrocyte apoptosis remains unclear. PUMA is a pro-apoptotic member of the BH3-only subgroup of the Bcl-2 family and is involved in a large number of physiological and pathological processes. In the present study, we examined whether PUMA has a role in IL-1β‑induced apoptosis and whether the c-Jun N-terminal kinase (JNK)/c-Jun pathway mediates the induction of PUMA, thus contributing to chondrocyte apoptosis. The results demonstrated an increase in PUMA protein and mRNA levels in cultured mouse chondrocytes following 4 h of IL-1β treatment. Furthermore, this upregulation of PUMA was critical for chondrocyte apoptosis as knockdown of PUMA using PUMA-specific siRNA significantly reduced apoptosis in cultured cells. Upon pharmacological inhibition of the JNK/c-Jun pathway with CE11004 or SP600125, the expression of PUMA was notably suppressed with a concomitant decrease in apoptosis observed in IL-1β-treated chondrocytes. Also, immunohistochemical studies revealed that the PUMA and c-Jun proteins were upregulated in chondrocytes from the articular cartilage of OA patients. Together, these data suggest a role for PUMA and the JNK/c-Jun pathway in the regulation of chondrocyte apoptosis during OA.
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Copy and paste a formatted citation
Spandidos Publications style
Lu H, Hou G, Zhang Y, Dai Y and Zhao H: c-Jun transactivates Puma gene expression to promote osteoarthritis. Mol Med Rep 9: 1606-1612, 2014.
APA
Lu, H., Hou, G., Zhang, Y., Dai, Y., & Zhao, H. (2014). c-Jun transactivates Puma gene expression to promote osteoarthritis. Molecular Medicine Reports, 9, 1606-1612. https://doi.org/10.3892/mmr.2014.1981
MLA
Lu, H., Hou, G., Zhang, Y., Dai, Y., Zhao, H."c-Jun transactivates Puma gene expression to promote osteoarthritis". Molecular Medicine Reports 9.5 (2014): 1606-1612.
Chicago
Lu, H., Hou, G., Zhang, Y., Dai, Y., Zhao, H."c-Jun transactivates Puma gene expression to promote osteoarthritis". Molecular Medicine Reports 9, no. 5 (2014): 1606-1612. https://doi.org/10.3892/mmr.2014.1981
Copy and paste a formatted citation
x
Spandidos Publications style
Lu H, Hou G, Zhang Y, Dai Y and Zhao H: c-Jun transactivates Puma gene expression to promote osteoarthritis. Mol Med Rep 9: 1606-1612, 2014.
APA
Lu, H., Hou, G., Zhang, Y., Dai, Y., & Zhao, H. (2014). c-Jun transactivates Puma gene expression to promote osteoarthritis. Molecular Medicine Reports, 9, 1606-1612. https://doi.org/10.3892/mmr.2014.1981
MLA
Lu, H., Hou, G., Zhang, Y., Dai, Y., Zhao, H."c-Jun transactivates Puma gene expression to promote osteoarthritis". Molecular Medicine Reports 9.5 (2014): 1606-1612.
Chicago
Lu, H., Hou, G., Zhang, Y., Dai, Y., Zhao, H."c-Jun transactivates Puma gene expression to promote osteoarthritis". Molecular Medicine Reports 9, no. 5 (2014): 1606-1612. https://doi.org/10.3892/mmr.2014.1981
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