Berberine induces double-strand DNA breaks in Rev3 deficient cells

Hu,Xiaojuan; Wu,Xiaohua; Huang,Yunfei; Tong,Qingyi; Takeda,Shunichi; Qing,Yong

doi:10.3892/mmr.2014.1999

Berberine induces double-strand DNA breaks in Rev3 deficient cells

Authors:
- Xiaojuan Hu
- Xiaohua Wu
- Yunfei Huang
- Qingyi Tong
- Shunichi Takeda
- Yong Qing
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Affiliations: Department of Pharmacology, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Regenerative Medicine Research Center, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Sichuan Academy of Chinese Medicine Sciences, Chengdu, Sichuan 610041, P.R. China, Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan
Published online on: February 28, 2014 https://doi.org/10.3892/mmr.2014.1999
Pages: 1883-1888

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Abstract

Berberine is a natural isoquinoline alkaloid, the majority of which is extracted from Huang Lian and other medicinal herbs. Numerous studies have revealed that berberine exhibits anticancer activity, however the mechanisms underlying this effect remain elusive. To examine these mechanisms, we analyzed the effects of berberine on a panel of DNA repair deficient chicken B lymphocyte (DT40) clones. Our results revealed that DT40 cells deficient in Rev3 (Rev3-/-), a translesion DNA synthesis (TLS) gene, were hypersensitive to berberine. Following berberine treatment, cell cycle analysis identified that G2/M arrest was increased in Rev3-/- cells. Furthermore, compared with wild-type cells (WT), berberine also induced a significant increase in double-strand breaks (DSBs) in Rev3-/- cells, as revealed by chromosomal aberration (CA) analysis. These results suggest that berberine is able to induce DNA damage, and that the Rev3 associated DNA repair pathway participates in the processes that aid its repair.

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