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Article

CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues

  • Authors:
    • Songshu Xiao
    • Yanhong Zhou
    • Jianfa Jiang
    • Le Yuan
    • Min Xue
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Molecular Genetics Laboratory, Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China
  • Pages: 1667-1674
    |
    Published online on: March 6, 2014
       https://doi.org/10.3892/mmr.2014.2010
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Abstract

Cervical carcinoma is the second most prevalent type of malignancy in females worldwide. The crucial etiological factors involved in the development of cervical carcinoma include infection with the papillomavirus, and the structural or functional mutation of oncogenes and tumor suppressor genes. CD44 refers to a multifunctional family of type I transmembrane proteins. These proteins have been implicated in numerous biological processes, including cell adhesion, cell migration and metastasis. The present study examined the differences in the expression levels of ATP‑binding cassette sub‑family G member 2, CD24, CD44, CD133, cytokeratin (CK) 14 and CK19 between cervical cancer tissues and corresponding normal non‑tumor tissues by flow cytometry. Then, the CD44+ or CD44‑ cells from cervical cancer tissues were sorted for identification and confirmation of differential expression by flow cytometry. The results demonstrated that the expression level of CD44 in cervical cancer tissues was higher than in the corresponding non‑tumor normal tissues (t=3.12; P=0.0102). Compared with the CD44‑ cells, the FOS‑like antigen 1 (Fra‑1), nestin, nuclear receptor subfamily 4, group A, member 2, OCT4 and p63 genes were highly expressed in CD44+ cells. The fold changes were 3.55, 3.55, 2.46, 2.87 and 2.56, respectively (P<0.05). However, BMI1 polycomb ring finger oncogene, ck5, tumor protein p53 and lactotransferrin genes exhibited low expression levels in CD44+ cells. It was verified by western blot analysis and flow cytometry that Fra‑1 was highly expressed in CD44+ cells. Fra‑1 was a potential target of miR‑19a and miR‑19b. The expression of miR‑19a and miR‑19b was downregulated by ~50% in CD44+ cells compared with CD44‑ cells. These findings suggested that CD44 dysregulated the activation of the Fra‑1 gene. The interaction of Fra‑1 and CD44 may therefore be important in cervical carcinoma.
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1 

Parish SL, Swaine JG, Son E and Luken K: Determinants of cervical cancer screening among women with intellectual disabilities: evidence from medical records. Public Health Rep. 128:519–526. 2013.PubMed/NCBI

2 

Wobus M, Kuns R, Wolf C, Horn LC, Köhler U, Sheyn I, Werness BA and Sherman LS: CD44 mediates constitutive type I receptor signaling in cervical carcinoma cells. Gynecol Oncol. 83:227–234. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Wu JH, Liang XA, Wu YM, Li FS and Dai YM: Identification of DNA methylation of SOX9 in cervical cancer using methylated-CpG island recovery assay. Oncol Rep. 29:125–132. 2013.PubMed/NCBI

4 

Dobo C, Stavale JN, de Lima FO, Ribeiro DA, Arias V, Gomes TS and Oshima CT: HSP27 is commonly expressed in cervical intraepithelial lesions of brazilian women. Asian Pac J Cancer Prev. 14:5007–5010. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Saavedra KP, Brebi PM and Roa JC: Epigenetic alterations in preneoplastic and neoplastic lesions of the cervix. Clin Epigenetics. 4:132012. View Article : Google Scholar : PubMed/NCBI

6 

Wang J, Wang Q, Liu H, Shao N, Tan B, Zhang G, Wang K, Jia Y, Ma W, Wang N and Cheng Y: The association of miR-146a rs2910164 and miR-196a2 rs11614913 polymorphisms with cancer risk: a meta-analysis of 32 studies. Mutagenesis. 27:779–788. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Makrydimas G, Zagorianakou N, Zagorianakou P and Agnantis NJ: CD44 family and gynaecological cancer. In Vivo. 17:633–640. 2003.PubMed/NCBI

8 

Ibrahim EM, Stewart RL, Corke K, Blackett AD, Tidy JA and Wells M: Upregulation of CD44 expression by interleukins 1, 4, and 13, transforming growth factor-beta1, estrogen, and progestogen in human cervical adenocarcinoma cell lines. Int J Gynecol Cancer. 16:1631–1642. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Speiser P, Wanner C, Tempfer C, Mittelböck M, Hanzal E, Bancher-Todesca D, Gitsch G, Reinthaller A and Kainz C: CD44 is an independent prognostic factor in early-stage cervical cancer. Int J Cancer. 74:185–188. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Mostaan LV, Khorsandi MT, Sharifian SM, Shandiz FH, Mirashrafi F, Sabzari H, Badiee R, Borghei H and Yazdani N: Correlation between E-cadherin and CD44 adhesion molecules expression and cervical lymph node metastasis in oral tongue SCC: Predictive significance or not. Pathol Res Pract. 207:448–451. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Oberprieler NG and Taskén K: Analysing phosphorylation-based signalling networks by phospho flow cytometry. Cell Signal. 23:14–18. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Zhou Y, Zeng Z, Zhang W, Xiong W, Wu M, Tan Y, Yi W, Xiao L, Li X, Huang C, et al: Lactotransferrin: a candidate tumor suppressor-deficient expression in human nasopharyngeal carcinoma and inhibition of NPC cell proliferation by modulating the mitogen-activated protein kinase pathway. Int J Cancer. 123:2065–2072. 2008. View Article : Google Scholar

13 

Krutzik PO, Irish JM, Nolan GP and Perez OD: Analysis of protein phosphorylation and cellular signaling events by flow cytometry: techniques and clinical applications. Clin Immunol. 110:206–221. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Zhang L, Tang A, Zhou Y, Tang J, Luo Z, Jiang C, Li X, Xiang J and Li G: Tumor-conditioned mesenchymal stem cells display hematopoietic differentiation and diminished influx of Ca2+. Stem Cells Dev. 21:1418–1428. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Zhou Y, Wang W, Zheng D, Peng S, Xiong W, Ma J, Zeng Z, Wu M, Zhou M, Xiang J, et al: Risk of nasopharyngeal carcinoma associated with polymorphic lactotransferrin haplotypes. Med Oncol. 29:1456–1462. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Salehi M, Taheri T, Mohit E, Zahedifard F, Seyed N, Taslimi Y, Sattari M, Bolhassani A and Rafati S: Recombinant Leishmania tarentolae encoding the HPV type 16 E7 gene in tumor mice model. Immunotherapy. 4:1107–1120. 2012.

18 

Sahiner F, Gümral R, Sener K, Yiğit N, Dede M, Yapar M and Kubar A: Investigation of HPV-DNA in cervical smear samples by two different methods: MY09/11 consensus PCR and type-specific real-time PCR. Mikrobiyol Bul. 46:624–636. 2012.(In Turkish).

19 

Balbi G, Napolitano A, Giordano F, Capuano S, Manganaro MA, Di Martino L, Fusco D, Grauso F and Seguino E: Role of the association of high-risk HPV identified by real-time PCR in cervical preneoplastic lesions. Eur J Gynaecol Oncol. 33:467–471. 2012.PubMed/NCBI

20 

Raychaudhuri S and Mandal S: Current status of knowledge, attitude and practice (KAP) and screening for cervical cancer in countries at different levels of development. Asian Pac J Cancer Prev. 13:4221–4227. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Gadducci A, Guerrieri ME and Greco C: Tissue biomarkers as prognostic variables of cervical cancer. Crit Rev Oncol Hematol. 86:104–129. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Mocarska A, Starosławska E, Zelazowska-Cieślińska I, Łosicki M, Stasiewicz D, Kieszko D and Burdan F: Epidemiology and risk factors of the cervical squamous cell carcinoma. Pol Merkur Lekarski. 33:101–106. 2012.(In Polish).

23 

Bourguignon LY, Wong G, Earle C and Chen L: Hyaluronan-CD44v3 interaction with Oct4-Sox2-Nanog promotes miR-302 expression leading to self-renewal, clonal formation, and cisplatin resistance in cancer stem cells from head and neck squamous cell carcinoma. J Biol Chem. 287:32800–32824. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Dhingra S, Feng W, Brown RE, Zhou Z, Khoury T, Zhang R and Tan D: Clinicopathologic significance of putative stem cell markers, CD44 and nestin, in gastric adenocarcinoma. Int J Clin Exp Pathol. 4:733–741. 2011.PubMed/NCBI

25 

Mascolo M, Ilardi G, Romano MF, Celetti A, Siano M, Romano S, Luise C, Merolla F, Rocco A, Vecchione ML, De Rosa G and Staibano S: Overexpression of chromatin assembly factor-1 p60, poly(ADP-ribose) polymerase 1 and nestin predicts metastasizing behaviour of oral cancer. Histopathology. 61:1089–1105. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, Miu K, Watnick RS, Reinhardt F, et al: Growth-inhibitory and tumor-suppressive functions of p53 depend on its repression of CD44 expression. Cell. 134:62–73. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Bidaud P, Chasle J, Sichel F, Rousseau S, Petit P, Pottier D, Picquenot JM, Louis MY and Lechevrel M: Expression of p53 family members and CD44 in oral squamous cell carcinoma (OSCC) in relation to tumorigenesis. Histol Histopathol. 25:331–339. 2010.PubMed/NCBI

28 

Ramos-Nino ME, Blumen SR, Pass H and Mossman BT: Fra-1 governs cell migration via modulation of CD44 expression in human mesotheliomas. Mol Cancer. 6:812007. View Article : Google Scholar : PubMed/NCBI

29 

Kajanne R, Miettinen P, Tenhunen M and Leppä S: Transcription factor AP-1 promotes growth and radioresistance in prostate cancer cells. Int J Oncol. 35:1175–1182. 2009.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xiao S, Zhou Y, Jiang J, Yuan L and Xue M: CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues. Mol Med Rep 9: 1667-1674, 2014.
APA
Xiao, S., Zhou, Y., Jiang, J., Yuan, L., & Xue, M. (2014). CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues. Molecular Medicine Reports, 9, 1667-1674. https://doi.org/10.3892/mmr.2014.2010
MLA
Xiao, S., Zhou, Y., Jiang, J., Yuan, L., Xue, M."CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues". Molecular Medicine Reports 9.5 (2014): 1667-1674.
Chicago
Xiao, S., Zhou, Y., Jiang, J., Yuan, L., Xue, M."CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues". Molecular Medicine Reports 9, no. 5 (2014): 1667-1674. https://doi.org/10.3892/mmr.2014.2010
Copy and paste a formatted citation
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Spandidos Publications style
Xiao S, Zhou Y, Jiang J, Yuan L and Xue M: CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues. Mol Med Rep 9: 1667-1674, 2014.
APA
Xiao, S., Zhou, Y., Jiang, J., Yuan, L., & Xue, M. (2014). CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues. Molecular Medicine Reports, 9, 1667-1674. https://doi.org/10.3892/mmr.2014.2010
MLA
Xiao, S., Zhou, Y., Jiang, J., Yuan, L., Xue, M."CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues". Molecular Medicine Reports 9.5 (2014): 1667-1674.
Chicago
Xiao, S., Zhou, Y., Jiang, J., Yuan, L., Xue, M."CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues". Molecular Medicine Reports 9, no. 5 (2014): 1667-1674. https://doi.org/10.3892/mmr.2014.2010
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