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Article

microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis

  • Authors:
    • Lili Zhou
    • Haitao Bai
    • Chun Wang
    • Daolin Wei
    • Youwen Qin
    • Xiaowei Xu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Shanghai Jiaotong University, Affiliated Shanghai First People's Hospital, Shanghai 200080, P.R. China
  • Pages: 1909-1916
    |
    Published online on: March 6, 2014
       https://doi.org/10.3892/mmr.2014.2011
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Abstract

microRNA‑125b (miR‑125b) is overexpressed in several types of cancer and contributes to tumor resistance to chemotherapy. The present study investigated the effect of miR‑125b on the resistance of leukemia cell lines to the chemotherapeutic agent daunorubicin (DNR). miR‑125b expression was found to be upregulated in patients who had failed therapy compared with those who demonstrated event‑free survival. The overexpression of miR‑125b was observed to induce DNR resistance in K562, THP‑1 and Jurkat cells by reducing apoptosis, whereas the suppression of miR‑125b was found to enhance DNR cytotoxicity in REH cells. Furthermore, miR‑125b was observed to mediate DNR resistance in leukemia cell lines through decreasing expression of G protein‑coupled receptor kinase 2 and p53‑upregulated modulator of apoptosis, which were shown to be direct targets of miR‑125b using a dual‑luciferase reporter. The present study provides a novel mechanism for understanding leukemia drug resistance and provides a novel method for calculating patient prognosis.
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1 

Kimby E, Nygren P and Glimelius B; SBU-group. Swedish Council of Technology Assessment in Health Care. A systematic overview of chemotherapy effects in acute myeloid leukaemia. Acta Oncol. 40:231–252. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Tallman MS, Gilliland DG and Rowe JM: Drug therapy for acute myeloid leukemia. Blood. 106:1154–1163. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Pulte D, Gondos A and Brenner H: Expected long-term survival of patients diagnosed with acute myeloblastic leukemia during 2006–2010. Ann Oncol. 21:335–341. 2010.

4 

Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Lim LP, Lau NC, Garrett-Engele P, et al: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature. 433:769–773. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Lu J, Getz G, Miska EA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Kim JK, Noh JH, Jung KH, et al: Sirtuin7 oncogenic potential in human hepatocellular carcinoma and its regulation by the tumor suppressors MiR-125a-5p and MiR-125b. Hepatology. 57:1055–1067. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Zhou M, Liu Z, Zhao Y, et al: MicroRNA-125b confers the resistance of breast cancer cells to paclitaxel through suppression of pro-apoptotic Bcl-2 antagonist killer 1 (Bak1) expression. J Biol Chem. 285:21496–21507. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Yuxia M, Zhennan T and Wei Z: Circulating miR-125b is a novel biomarker for screening non-small-cell lung cancer and predicts poor prognosis. J Cancer Res Clin Oncol. 138:2045–2050. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Kappelmann M, Kuphal S, Meister G, Vardimon L and Bosserhoff AK: MicroRNA miR-125b controls melanoma progression by direct regulation of c-Jun protein expression. Oncogene. 32:2984–2991. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Nishida N, Yokobori T, Mimori K, et al: MicroRNA miR-125b is a prognostic marker in human colorectal cancer. Int J Oncol. 38:1437–1443. 2011.PubMed/NCBI

13 

Bousquet M, Quelen C, Rosati R, et al: Myeloid cell differentiation arrest by miR-125b-1 in myelodysplastic syndrome and acute myeloid leukemia with the t(2;11)(p21;q23) translocation. J Exp Med. 205:2499–2506. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Enomoto Y, Kitaura J, Hatakeyama K, et al: Eμ/miR-125b transgenic mice develop lethal B-cell malignancies. Leukemia. 25:1849–1856. 2011.

15 

Klusmann JH, Li Z, Böhmer K, et al: miR-125b-2 is a potential oncomiR on human chromosome 21 in megakaryoblastic leukemia. Genes Dev. 24:478–490. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Gefen N, Binder V, Zaliova M, et al: Hsa-mir-125b-2 is highly expressed in childhood ETV6/RUNX1 (TEL/AML1) leukemias and confers survival advantage to growth inhibitory signals independent of p53. Leukemia. 24:89–96. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Calin GA, Liu CG, Sevignani C, et al: MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proc Natl Acad Sci USA. 101:11755–11760. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Zhang H, Luo XQ, Feng DD, et al: Upregulation of microRNA-125b contributes to leukemogenesis and increases drug resistance in pediatric acute promyelocytic leukemia. Mol Cancer. 10:1082011. View Article : Google Scholar : PubMed/NCBI

19 

Schotte D, De Menezes RX, Akbari Moqadam F, et al: MicroRNA characterize genetic diversity and drug resistance in pediatric acute lymphoblastic leukemia. Haematologica. 96:703–711. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Döhner H, Estey EH, Amadori S, et al; European LeukemiaNet. Diagnosis and management of acute myeloid leukemia in adults: recommendations from an international expert panel, on behalf of the European LeukemiaNet. Blood. 115:453–474. 2010.PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Starczynowski DT, Kuchenbauer F, Argiropoulos B, et al: Identification of miR-145 and miR-146a as mediators of the 5q-syndrome phenotype. Nat Med. 16:49–58. 2010. View Article : Google Scholar

23 

Hughes MS, Yu YY, Dudley ME, et al: Transfer of a TCR gene derived from a patient with a marked antitumor response conveys highly active T-cell effector functions. Hum Gene Ther. 16:457–472. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Stark A, Brennecke J, Russell RB and Cohen SM: Identification of Drosophila MicroRNA targets. PLoS Biol. 1:397–409. 2003.

25 

Husted S, Søkilde R, Rask L, et al: MicroRNA expression profiles associated with development of drug resistance in Ehrlich ascites tumor cells. Mol Pharm. 8:2055–2062. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Rajabi H, Jin C, Ahmad R, McClary C, Joshi MD and Kufe D: Mucin 1 oncoprotein expression is suppressed by the miR-125b oncomir. Genes Cancer. 1:62–68. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Iida K, Fukushi J, Matsumoto Y, et al: miR-125b develops chemoresistance in Ewing sarcoma/primitive neuroectodermal tumor. Cancer Cell Int. 13:212013. View Article : Google Scholar : PubMed/NCBI

28 

Kong F, Sun C, Wang Z, et al: miR-125b confers resistance of ovarian cancer cells to cisplatin by targeting pro-apoptotic Bcl-2 antagonist killer 1. J Huazhong Univ Sci Technolog Med Sci. 31:543–549. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Richardson DS and Johnson SA: Anthracyclines in haematology: preclinical studies, toxicity and delivery systems. Blood Rev. 11:201–223. 1997. View Article : Google Scholar : PubMed/NCBI

30 

Gong J, Zhang JP, Li B, et al: MicroRNA-125b promotes apoptosis by regulating the expression of Mcl-1, Bcl-w and IL-6R. Oncogene. 32:3071–3079. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Shi L, Zhang S, Feng K, et al: MicroRNA-125b-2 confers human glioblastoma stem cells resistance to temozolomide through the mitochondrial pathway of apoptosis. Int J Oncol. 40:119–129. 2012.PubMed/NCBI

32 

Balakrishnan A, Stearns AT, Park PJ, et al: Upregulation of proapoptotic microRNA mir-125a after massive small bowel resection in rats. Ann Surg. 255:747–753. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Zeng CW, Zhang XJ, Lin KY, et al: Camptothecin induces apoptosis in cancer cells via microRNA-125b-mediated mitochondrial pathways. Mol Pharmacol. 81:578–586. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Jiang F, Liu T, He Y, et al: MiR-125b promotes proliferation and migration of type II endometrial carcinoma cells through targeting TP53INP1 tumor suppressor in vitro and in vivo. BMC Cancer. 11:4252011. View Article : Google Scholar : PubMed/NCBI

35 

Kim SW, Ramasamy K, Bouamar H, Lin AP, Jiang D and Aguiar RC: MicroRNAs miR-125a and miR-125b constitutively activate the NF-κB pathway by targeting the tumor necrosis factor alpha-induced protein 3 (TNFAIP3, A20). Proc Natl Acad Sci USA. 109:7865–7870. 2012.PubMed/NCBI

36 

Tan G, Niu J, Shi Y, Ouyang H and Wu ZH: NF-κB-dependent microRNA-125b up-regulation promotes cell survival by targeting p38α upon ultraviolet radiation. J Biol Chem. 287:33036–33047. 2012.

37 

Bousquet M, Nguyen D, Chen C, et al: MicroRNA-125b transforms myeloid cell lines by repressing multiple mRNA. Haematologica. 97:1713–1721. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou L, Bai H, Wang C, Wei D, Qin Y and Xu X: microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis. Mol Med Rep 9: 1909-1916, 2014.
APA
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., & Xu, X. (2014). microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis. Molecular Medicine Reports, 9, 1909-1916. https://doi.org/10.3892/mmr.2014.2011
MLA
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., Xu, X."microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis". Molecular Medicine Reports 9.5 (2014): 1909-1916.
Chicago
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., Xu, X."microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis". Molecular Medicine Reports 9, no. 5 (2014): 1909-1916. https://doi.org/10.3892/mmr.2014.2011
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou L, Bai H, Wang C, Wei D, Qin Y and Xu X: microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis. Mol Med Rep 9: 1909-1916, 2014.
APA
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., & Xu, X. (2014). microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis. Molecular Medicine Reports, 9, 1909-1916. https://doi.org/10.3892/mmr.2014.2011
MLA
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., Xu, X."microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis". Molecular Medicine Reports 9.5 (2014): 1909-1916.
Chicago
Zhou, L., Bai, H., Wang, C., Wei, D., Qin, Y., Xu, X."microRNA‑125b promotes leukemia cell resistance to daunorubicin by inhibiting apoptosis". Molecular Medicine Reports 9, no. 5 (2014): 1909-1916. https://doi.org/10.3892/mmr.2014.2011
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