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Molecular Medicine Reports
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Article

Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease

  • Authors:
    • Jie Mi
    • Dong Chen
    • Mei Wu
    • Weilin Wang
    • Hong Gao
  • View Affiliations / Copyright

    Affiliations: Laboratory of Congenital Malformation, Ministry of Public Health, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 1839-1843
    |
    Published online on: March 6, 2014
       https://doi.org/10.3892/mmr.2014.2022
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Abstract

Hirschsprung's disease (HSCR) is a polygenic disease, of which the cause remains to be elucidated. It has been suggested that SRY‑related HMG‑box 9 (SOX9) is fundamental for the correct development of oligodendrocytes and astrocytes; however, not the development of neurons. There are currently no reports regarding SOX9 expression in patients with HSCR; therefore, the present study aimed to investigate the expression of microRNA‑124 (miR‑124) and its target gene, SOX9, in HSCR. Quantitative polymerase chain reaction (qPCR), western blot analysis and immunohistochemistry were used to detect the mRNA and protein expression of miR‑124 and SOX9 in patients with HSCR. miR‑124 expression was observed to be markedly higher in stenotic colon segment tissues compared with normal colon segment tissues in patients with HSCR. Furthermore, mRNA and protein analyses revealed that SOX9 expression was also higher in the stenotic colon segment tissues compared with the normal colon segment tissues. In conclusion, these data suggest that miR‑124 and its target gene, SOX9, are overexpressed in the stenotic colon segment of patients with HSCR, and may have a significant role in the development of HSCR.
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1 

Amiel J and Lyonnet S: Hirschsprung disease, associated syndromes, and genetics: a review. J Med Genet. 38:729–739. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Whitehouse FR and Kernohan JW: Myenteric plexus in congenital megacolon; study of 11 cases. Arch Intern Med (Chic). 82:75–111. 1948. View Article : Google Scholar : PubMed/NCBI

3 

Haricharan RN and Georgeson KE: Hirschsprung disease. Semin Pediatr Surg. 17:266–275. 2008. View Article : Google Scholar

4 

Tam PK and Garcia-Barceló M: Genetic basis of Hirschsprung’s disease. Pediatr Surg Int. 25:543–558. 2009.

5 

Angrist M, Kauffman E, Slaugenhaupt SA, et al: A gene for Hirschsprung disease (megacolon) in the pericentromeric region of human chromosome 10. Nat Genet. 4:351–356. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Luo Y, Ceccherini I, Pasini B, et al: Close linkage with the RET protooncogene and boundaries of deletion mutations in autosomal dominant Hirschsprung disease. Hum Mol Genet. 2:1803–1808. 1993. View Article : Google Scholar : PubMed/NCBI

7 

Lyonnet S, Bolino A, Pelet A, et al: A gene for Hirschsprung disease maps to the proximal long arm of chromosome 10. Nat Genet. 4:346–350. 1993. View Article : Google Scholar : PubMed/NCBI

8 

Amiel J, Attié T, Jan D, et al: Heterozygous endothelin receptor B (EDNRB) mutations in isolated Hirschsprung disease. Hum Mol Genet. 5:355–357. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Attié T, Till M, Pelet A, Amiel J, Edery P, Boutrand L, Munnich A and Lyonnet S: Mutation of the endothelin-receptor B gene in Waardenburg-Hirschsprung disease. Hum Mol Genet. 4:2407–2409. 1995.PubMed/NCBI

10 

Kusafuka T, Wang Y and Puri P: Novel mutations of the endothelin-B receptor gene in isolated patients with Hirschsprung’s disease. Hum Mol Genet. 5:347–349. 1996.

11 

Edery P, Attié T, Amiel J, Pelet A, Eng C, Hofstra RM, Martelli H, Bidaud C, Munnich A and Lyonnet S: Mutation of the endothelin-3 gene in the Waardenburg-Hirschsprung disease (Shah-Waardenburg syndrome). Nat Genet. 12:442–444. 1996. View Article : Google Scholar : PubMed/NCBI

12 

Hofstra RM, Osinga J, Tan-Sindhunata G, et al: A homozygous mutation in the endothelin-3 gene associated with a combined Waardenburg type 2 and Hirschsprung phenotype (Shah-Waardenburg syndrome). Nat Genet. 12:445–447. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Angrist M, Bolk S, Halushka M, Lapchak PA and Chakravarti A: Germline mutations in glial cell line-derived neurotrophic factor (GDNF) and RET in a Hirschsprung disease patient. Nat Genet. 14:341–344. 1996. View Article : Google Scholar : PubMed/NCBI

14 

Ivanchuk SM, Myers SM, Eng C and Mulligan LM: De novo mutation of GDNF, ligand for the RET/GDNFR-alpha receptor complex, in Hirschsprung disease. Hum Mol Genet. 5:2023–2026. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Salomon R, Attié T, Pelet A, et al: Germline mutations of the RET ligand GDNF are not sufficient to cause Hirschsprung disease. Nat Genet. 14:345–347. 1996. View Article : Google Scholar : PubMed/NCBI

16 

Pingault V, Bondurand N, Kuhlbrodt K, et al: SOX10 mutations in patients with Waardenburg-Hirschsprung disease. Nat Genet. 18:171–173. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Southard-Smith EM, Angrist M, Ellison JS, Agarwala R, Baxevanis AD, Chakravarti A and Pavan WJ: The Sox10(Dom) mouse: modeling the genetic variation of Waardenburg-Shah (WS4) syndrome. Genome Res. 9:215–225. 1999.PubMed/NCBI

18 

Sham MH, Lui VC, Fu M, Chen B and Tam PK: SOX10 is abnormally expressed in aganglionic bowel of Hirschsprung’s disease infants. Gut. 49:220–226. 2001.PubMed/NCBI

19 

Stolt CC, Lommes P, Sock E, Chaboissier MC, Schedl A and Wegner M: The Sox9 transcription factor determines glial fate choice in the developing spinal cord. Genes Dev. 17:1677–1689. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Kordes U, Cheng YC and Scotting PJ: Sox group E gene expression distinguishes different types and maturational stages of glial cells in developing chick and mouse. Brain Res Dev Brain Res. 157:209–213. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Kloosterman WP and Plasterk RH: The diverse functions of microRNAs in animal development and disease. Dev Cell. 11:441–450. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Cheng LC, Pastrana E, Tavazoie M and Doetsch F: miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche. Nat Neurosci. 12:399–408. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Grandjean V, Gounon P, Wagner N, Martin L, Wagner KD, Bernex F, Cuzin F and Rassoulzadegan M: The miR-124-Sox9 paramutation: RNA-mediated epigenetic control of embryonic and adult growth. Development. 136:3647–3655. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Simic P and Vukicevic S: Bone morphogenetic proteins in development and homeostasis of kidney. Cytokine Growth Factor Rev. 6:299–308. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Martucciello G: Hirschsprung’s disease, one of the most difficult diagnoses in pediatric surgery: a review of the problems from clinical practice to the bench. Eur J Pediatr Surg. 18:140–149. 2008.

27 

Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

28 

van Rooij E: The art of microRNA research. Circ Res. 108:219–234. 2011.

29 

Papagiannakopoulos T and Kosik KS: MicroRNA-124: micromanager of neurogenesis. Cell Stem Cell. 4:375–376. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Mi J, Chen D, Wu M, Wang W and Gao H: Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease. Mol Med Rep 9: 1839-1843, 2014.
APA
Mi, J., Chen, D., Wu, M., Wang, W., & Gao, H. (2014). Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease. Molecular Medicine Reports, 9, 1839-1843. https://doi.org/10.3892/mmr.2014.2022
MLA
Mi, J., Chen, D., Wu, M., Wang, W., Gao, H."Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease". Molecular Medicine Reports 9.5 (2014): 1839-1843.
Chicago
Mi, J., Chen, D., Wu, M., Wang, W., Gao, H."Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease". Molecular Medicine Reports 9, no. 5 (2014): 1839-1843. https://doi.org/10.3892/mmr.2014.2022
Copy and paste a formatted citation
x
Spandidos Publications style
Mi J, Chen D, Wu M, Wang W and Gao H: Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease. Mol Med Rep 9: 1839-1843, 2014.
APA
Mi, J., Chen, D., Wu, M., Wang, W., & Gao, H. (2014). Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease. Molecular Medicine Reports, 9, 1839-1843. https://doi.org/10.3892/mmr.2014.2022
MLA
Mi, J., Chen, D., Wu, M., Wang, W., Gao, H."Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease". Molecular Medicine Reports 9.5 (2014): 1839-1843.
Chicago
Mi, J., Chen, D., Wu, M., Wang, W., Gao, H."Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease". Molecular Medicine Reports 9, no. 5 (2014): 1839-1843. https://doi.org/10.3892/mmr.2014.2022
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