KLF8 knockdown suppresses proliferation and invasion in human osteosarcoma cells

Lin,Feng; Shen,Zan; Tang,Li‑Na; Zheng,Shui‑Er; Sun,Yuan‑Jue; Min,Da‑Liu; Yao,Yang

doi:10.3892/mmr.2014.2027

May-2014 Volume 9 Issue 5

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May-2014 Volume 9 Issue 5

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KLF8 knockdown suppresses proliferation and invasion in human osteosarcoma cells

Authors:
- Feng Lin
- Zan Shen
- Li‑Na Tang
- Shui‑Er Zheng
- Yuan‑Jue Sun
- Da‑Liu Min
- Yang Yao
View Affiliations / Copyright

Affiliations: Graduate School of Medicine, Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China

Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1613-1617
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Published online on: March 7, 2014

https://doi.org/10.3892/mmr.2014.2027
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Abstract

Krüppel‑like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT). EMT is a key process in tumor metastasis. Although overexpression of KLF8 has been observed in a variety of human tumor types, the role of KLF8 in human osteosarcoma is yet to be elucidated. The present study aimed to investigate the biological impact of KLF8 on Saos‑2 osteosarcoma cells. KLF8 gene expression was knocked down in vitro using a lentivirus‑mediated small interfering (si)RNA method. Cell proliferation and cell cycle distribution were evaluated using 3-(4,5)-dimethylthiahiazo(‑z-yl)-3,5-di-phenytetrazoliumromide and colony formation assays, and flow cytometry, respectively. Cell invasion was analyzed using a Transwell® invasion assay. Knockdown of KLF8 was found to significantly inhibit proliferation and invasion in osteosarcoma cells. These data suggest that KLF8 may exhibit an important role in osteosarcoma tumorigenesis and that KLF8 may be a potential therapeutic target for the treatment of osteosarcoma.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

KLF8 knockdown suppresses proliferation and invasion in human osteosarcoma cells

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Abstract

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