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Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells

  • Authors:
    • Fei Li
    • Ke Xia
    • Md Sayed Ali Sheikh
    • Jinfang Cheng
    • Chuanchang Li
    • Tianlun Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1634-1640
    |
    Published online on: March 7, 2014
       https://doi.org/10.3892/mmr.2014.2028
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Abstract

Recent studies have suggested that retinol binding protein 4 (RBP4), an adipocytokine related to insulin resistance (IR), may play an important role in the development of atherosclerosis and cardiovascular diseases (CVD). Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) is one of the most common causes of atherosclerosis. Hyperinsulinism promotes proliferation of VSMCs through the MAPK pathway. However, whether RBP4 is involved in insulin-induced proliferation of VSMCs leading to atherosclerosis remains unclear. In the present study, we evaluated the role of RBP4 and the potential relevance of signaling pathways in this process. Different concentrations of RBP4 (1 and 4 µg/ml) were added to rat aortic smooth muscle cells (RASMCs) during insulin-induced proliferation. The levels of cell growth signaling pathway proteins ERK1/2, p-ERK1/2, JAK2, p-JAK2, STAT3 and p-STAT3 were assessed by western blotting in order to identify the pathway(s) that are activated during insulin-induced proliferation. The specific inhibitors of ERK1/2 (PD98059) and JAK2 (AG490) were used to confirm our findings. Insulin induced proliferation of RASMCs in a concentration- and time-dependent manner, and increased the expression of ERK1/2, p-ERK1/2, JAK2, p-JAK2, STAT3 and p-STAT3 in a time-dependent manner. RBP4 enhanced insulin-induced proliferation of RASMCs and expression of p-ERK1/2 and p-JAK2. RBP4‑induced proliferation of RASMCs was reduced by the ERK1/2 inhibitor, while it was unaffected by the JAK2 inhibitor. These results suggest that RBP4 mediates VSMC proliferation induced by insulin via activation of the MAPK pathway, and highlight RBP4 as a modulator of atherosclerosis in hyperinsulinemia, therby enhancing our understanding on a number of unexpected aspects of CVD.
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Copy and paste a formatted citation
Spandidos Publications style
Li F, Xia K, Sheikh MA, Cheng J, Li C and Yang T: Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells. Mol Med Rep 9: 1634-1640, 2014.
APA
Li, F., Xia, K., Sheikh, M.A., Cheng, J., Li, C., & Yang, T. (2014). Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells. Molecular Medicine Reports, 9, 1634-1640. https://doi.org/10.3892/mmr.2014.2028
MLA
Li, F., Xia, K., Sheikh, M. A., Cheng, J., Li, C., Yang, T."Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells". Molecular Medicine Reports 9.5 (2014): 1634-1640.
Chicago
Li, F., Xia, K., Sheikh, M. A., Cheng, J., Li, C., Yang, T."Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells". Molecular Medicine Reports 9, no. 5 (2014): 1634-1640. https://doi.org/10.3892/mmr.2014.2028
Copy and paste a formatted citation
x
Spandidos Publications style
Li F, Xia K, Sheikh MA, Cheng J, Li C and Yang T: Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells. Mol Med Rep 9: 1634-1640, 2014.
APA
Li, F., Xia, K., Sheikh, M.A., Cheng, J., Li, C., & Yang, T. (2014). Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells. Molecular Medicine Reports, 9, 1634-1640. https://doi.org/10.3892/mmr.2014.2028
MLA
Li, F., Xia, K., Sheikh, M. A., Cheng, J., Li, C., Yang, T."Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells". Molecular Medicine Reports 9.5 (2014): 1634-1640.
Chicago
Li, F., Xia, K., Sheikh, M. A., Cheng, J., Li, C., Yang, T."Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cells". Molecular Medicine Reports 9, no. 5 (2014): 1634-1640. https://doi.org/10.3892/mmr.2014.2028
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