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Article

β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes

  • Authors:
    • Sen Wang
    • Di Xu
    • Ting‑Ting Wu
    • Yan Guo
    • Yan‑Hong Chen
    • Jian‑Gang Zou
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 1923-1928
    |
    Published online on: March 10, 2014
       https://doi.org/10.3892/mmr.2014.2035
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Abstract

Human ether‑à‑go‑go‑related gene (hERG) potassium channels conduct the rapid component of the delayed rectifier potassium current (IKr), which is crucial for repolarization of cardiac action potential. Patients with hERG‑associated long QT syndrome usually develop tachyarrhythmias during physical and/or emotional stress, both known to stimulate adrenergic receptors. The present study aimed to investigate a putative functional link between β1‑adrenergic stimulation and IKr in guinea‑pig left ventricular myocytes and to analyze how IKr is regulated following activation of the β1‑adrenergic signaling pathway. The IKr current was measured using a whole‑cell patch‑clamp technique. A selective β1‑adrenergic receptor agonist, xamoterol, at concentrations of 0.01‑100 µM decreased IKr in a concentration‑dependent manner. The 10 µM xamoterol‑induced inhibition of IKr was attenuated by the protein kinase A (PKA) inhibitor KT5720, the protein kinase C (PKC) inhibitor chelerythrine, and the phospholipase   (PLC) inhibitor U73122, indicating involvement of PKA, PKC and PLC in β1‑adrenergic inhibition of IKr. The results of the present study indicate an association between IKr and the β1‑adrenergic receptor in arrhythmogenesis, involving the activation of PKA, PKC and PLC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Xu D, Wu TT, Guo Y, Chen YH and Zou JG: β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes. Mol Med Rep 9: 1923-1928, 2014.
APA
Wang, S., Xu, D., Wu, T., Guo, Y., Chen, Y., & Zou, J. (2014). β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes. Molecular Medicine Reports, 9, 1923-1928. https://doi.org/10.3892/mmr.2014.2035
MLA
Wang, S., Xu, D., Wu, T., Guo, Y., Chen, Y., Zou, J."β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes". Molecular Medicine Reports 9.5 (2014): 1923-1928.
Chicago
Wang, S., Xu, D., Wu, T., Guo, Y., Chen, Y., Zou, J."β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes". Molecular Medicine Reports 9, no. 5 (2014): 1923-1928. https://doi.org/10.3892/mmr.2014.2035
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Xu D, Wu TT, Guo Y, Chen YH and Zou JG: β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes. Mol Med Rep 9: 1923-1928, 2014.
APA
Wang, S., Xu, D., Wu, T., Guo, Y., Chen, Y., & Zou, J. (2014). β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes. Molecular Medicine Reports, 9, 1923-1928. https://doi.org/10.3892/mmr.2014.2035
MLA
Wang, S., Xu, D., Wu, T., Guo, Y., Chen, Y., Zou, J."β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes". Molecular Medicine Reports 9.5 (2014): 1923-1928.
Chicago
Wang, S., Xu, D., Wu, T., Guo, Y., Chen, Y., Zou, J."β1‑adrenergic regulation of rapid component of delayed rectifier K+ currents in guinea‑pig cardiac myocytes". Molecular Medicine Reports 9, no. 5 (2014): 1923-1928. https://doi.org/10.3892/mmr.2014.2035
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