Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2014 Volume 9 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2014 Volume 9 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

  • Authors:
    • Gentaro Goto
    • Yoko Hori
    • Masashi Ishikawa
    • Shunsuke Tanaka
    • Atsuhiro Sakamoto
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology and Pain Medicine, Graduate School of Medicine, Nippon Medical School, Tokyo 113-8603, Japan
  • Pages: 1715-1722
    |
    Published online on: March 11, 2014
       https://doi.org/10.3892/mmr.2014.2038
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

General anesthesia is commonly used in the surgical arena, but little is known regarding its influence at the genomic and molecular levels. MicroRNAs (miRNAs) belong to a new class of non-coding RNA molecules which influence cell biology. In the present study, it was hypothesized that miRNAs alter gene expression levels under general anesthesia. The aim was to compare the miRNA expression profiles in the rat hippocampus in response to anesthesia with representative volatile (sevoflurane) and intravenous (propofol) anesthetics. Wistar Rats were randomly assigned to either a 2.4% sevoflurane, 600 µg/kg/min propofol or control (without anesthetics) group. Total RNA from hippocampal samples which contained miRNA was subjected to quantitative reverse transcription-polymerase chain reaction and Taqman Low-Density Arrays (TLDA). A total of 373 miRNAs are associated with rats and the TLDA analysis revealed that 279 expressed miRNAs (74.8%) were expressed in all three groups. Significant differences in the levels of 33 of the 279 expressed miRNAs (11.8%) were observed among the three groups in response to the anesthetic agents, and when compared with the control group, significant differences were found in 26 of the 279 expressed miRNAs (9.3%). Following sevoflurane anesthesia, the levels of four miRNAs were significantly increased and those of 12 were significantly reduced. By contrast, following propofol anesthesia, the levels of 11 miRNAs were significantly reduced but no miRNAs exhibited significantly elevated levels. One miRNA was common between the two anesthesia groups, whereas 14 miRNAs were significantly differentially expressed. In conclusion, sevoflurane and propofol exerted different effects on miRNA expression in the rat hippocampus.
View Figures

Figure 1

Figure 2

View References

1 

Forrest JB, Rehder K, Goldsmith CH, et al: Multicenter study of general anesthesia. I. Design and patient demography. Anesthesiology. 72:252–261. 1990. View Article : Google Scholar : PubMed/NCBI

2 

Brown BR Jr and Frink EJ: The safety of sevoflurane in humans. Anesthesiology. 79:201–203. 1993. View Article : Google Scholar : PubMed/NCBI

3 

Marik PE: Propofol: therapeutic indications and side-effects. Curr Pharm Des. 10:3639–3649. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Stachnik J: Inhaled anesthetic agents. Am J Health Syst Pharm. 63:623–634. 2006. View Article : Google Scholar

5 

Eger EI 2nd: Characteristics of anesthetic agents used for induction and maintenance of general anesthesia. Am J Health Syst Pharm. 61(Suppl 4): S3–S10. 2004.PubMed/NCBI

6 

Simons PJ, Cockshott ID, Douglas EJ, Gordon EA, Hopkins K and Rowland M: Disposition in male volunteers of a subanaesthetic intravenous dose of an oil in water emulsion of 14C-propofol. Xenobiotica. 18:429–440. 1988. View Article : Google Scholar : PubMed/NCBI

7 

Sakamoto A, Imai J, Nishikawa A, et al: Influence of inhalation anesthesia assessed by comprehensive gene expression profiling. Gene. 356:39–48. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Kobayashi K, Takemori K and Sakamoto A: Circadian gene expression is suppressed during sevoflurane anesthesia and the suppression persists after awakening. Brain Res. 1185:1–7. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Yoshida Y, Nakazato K, Takemori K, Kobayashi K and Sakamoto A: The influences of propofol and dexmedetomidine on circadian gene expression in rat brain. Brain Res Bull. 79:441–444. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Tsuboko Y and Sakamoto A: Propofol anaesthesia alters the cerebral proteome differently from sevoflurane anaesthesia. Biomed Res. 32:55–65. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Kawaguchi H, Hirakawa K, Miyauchi K, Koike K, Ohno Y and Sakamoto A: Pattern recognition analysis of proton nuclear magnetic resonance spectra of brain tissue extracts from rats anesthetized with propofol or isoflurane. PLoS One. 5:e111722010. View Article : Google Scholar

12 

Bak M, Silahtaroglu A, Møller M, et al: MicroRNA expression in the adult mouse central nervous system. RNA. 14:432–444. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Miranda KC, Huynh T, Tay Y, et al: A pattern-based method for the identification of MicroRNA binding sites and their corresponding heteroduplexes. Cell. 126:1203–1217. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Brennecke J, Stark A, Russell RB and Cohen SM: Principles of microRNA-target recognition. PLoS Biol. 3:e852005. View Article : Google Scholar : PubMed/NCBI

15 

Wayman GA, Davare M, Ando H, et al: An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP. Proc Natl Acad Sci USA. 105:9093–9098. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Visvanathan J, Lee S, Lee B, Lee JW and Lee SK: The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development. Genes Dev. 21:744–749. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Bredy TW, Lin Q, Wei W, Baker-Andresen D and Mattick JS: MicroRNA regulation of neural plasticity and memory. Neurobiol Learn Mem. 96:89–94. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Lee K, Kim JH, Kwon OB, et al: An activity-regulated microRNA, miR-188, controls dendritic plasticity and synaptic transmission by downregulating neuropilin-2. J Neurosci. 32:5678–5687. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Kosik KS: The neuronal microRNA system. Nat Rev Neurosci. 7:911–920. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Pan Z, Lu XF, Shao C, et al: The effects of sevoflurane anesthesia on rat hippocampus: a genomic expression analysis. Brain Res. 1381:124–133. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Ishikawa M, Tanaka S, Arai M, Genda Y and Sakamoto A: differences in microRNA changes of healthy rat liver between sevoflurane and propofol anesthesia. Anesthesiology. 117:1245–1252. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Tanaka S, Ishikawa M, Arai M, Genda Y and Sakamoto A: Changes in microRNA expression in rat lungs caused by sevoflurane anesthesia: a TaqMan® low-density array study. Biomed Res. 33:255–263. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Genda Y, Arai M, Ishikawa M, Tanaka S, Okabe T and Sakamoto A: microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TaqMan® Low Density Array study. Int J Mol Med. 31:129–137. 2013.PubMed/NCBI

24 

Ethical standards for investigations of experimental pain in animals. The Committee for Research and Ethical Issues of the International Association for the Study of Pain. Pain. 9:141–143. 1980. View Article : Google Scholar : PubMed/NCBI

25 

Obal D, Preckel B, Scharbatke H, et al: One MAC of sevoflurane provides protection against reperfusion injury in the rat heart in vivo. Br J Anaesth. 87:905–911. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Orth M, Barter L, Dominguez C, Atherley R, Carstens E and Antognini JF: Halothane and propofol differentially affect electroencephalographic responses to noxious stimulation. Br J Anaesth. 95:477–484. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Chiu K, Lau WM, Lau HT, So KF and Chang RC: Micro-dissection of rat brain for RNA or protein extraction from specific brain region. J Vis Exp. 7:2692007.PubMed/NCBI

28 

Glowinski J and Iversen LL: Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain. J Neurochem. 13:655–669. 1966.

29 

Wang B, Howel P, Bruheim S, et al: Systematic evaluation of three microRNA profiling platforms: microarray, beads array, and quantitative real-time PCR array. PLoS One. 6:e171672011. View Article : Google Scholar : PubMed/NCBI

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001.

31 

Skalnikova H, Vodicka P, Gadher SJ and Kovarova H: Proteomics of neural stem cells. Expert Rev Proteomics. 5:175–186. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Cui Y, Xiao Z, Han J, et al: MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin. BMC Neurosci. 13:1162012. View Article : Google Scholar : PubMed/NCBI

33 

Saba R, Störchel PH, Aksoy-Aksel A, et al: Dopamine-regulated microRNA MiR-181a controls GluA2 surface expression in hippocampal neurons. Mol Cell Biol. 32:619–632. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Liu Y, Zhao Z, Yang F, Gao Y, Song J and Wan Y: microRNA-181a is involved in insulin-like growth factor-1-mediated regulation of the transcription factor CREB1. J Neurochem. 126:771–780. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Mozzachiodi R and Byrne JH: More than synaptic plasticity: role of nonsynaptic plasticity in learning and memory. Trends Neurosci. 33:17–26. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Hui AB, Shi W, Boutros PC, et al: Robust global micro-RNA profiling with formalin-fixed paraffin-embedded breast cancer tissues. Lab Invest. 89:597–606. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Mees ST, Schleicher C, Mardin WA, Senninger N, Colombo-Benkmann M and Haier J: Analyzing miRNAs in ductal adenocarcinomas of the pancreas. J Surg Res. 169:241–246. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Arai M, Genda Y, Ishikawa M, Shunsuke T, Okabe T and Sakamoto A: The miRNA and mRNA changes in rat hippocampi after chronic constriction injury. Pain Med. 14:720–729. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Goto G, Hori Y, Ishikawa M, Tanaka S and Sakamoto A: Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia. Mol Med Rep 9: 1715-1722, 2014.
APA
Goto, G., Hori, Y., Ishikawa, M., Tanaka, S., & Sakamoto, A. (2014). Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia. Molecular Medicine Reports, 9, 1715-1722. https://doi.org/10.3892/mmr.2014.2038
MLA
Goto, G., Hori, Y., Ishikawa, M., Tanaka, S., Sakamoto, A."Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia". Molecular Medicine Reports 9.5 (2014): 1715-1722.
Chicago
Goto, G., Hori, Y., Ishikawa, M., Tanaka, S., Sakamoto, A."Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia". Molecular Medicine Reports 9, no. 5 (2014): 1715-1722. https://doi.org/10.3892/mmr.2014.2038
Copy and paste a formatted citation
x
Spandidos Publications style
Goto G, Hori Y, Ishikawa M, Tanaka S and Sakamoto A: Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia. Mol Med Rep 9: 1715-1722, 2014.
APA
Goto, G., Hori, Y., Ishikawa, M., Tanaka, S., & Sakamoto, A. (2014). Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia. Molecular Medicine Reports, 9, 1715-1722. https://doi.org/10.3892/mmr.2014.2038
MLA
Goto, G., Hori, Y., Ishikawa, M., Tanaka, S., Sakamoto, A."Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia". Molecular Medicine Reports 9.5 (2014): 1715-1722.
Chicago
Goto, G., Hori, Y., Ishikawa, M., Tanaka, S., Sakamoto, A."Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia". Molecular Medicine Reports 9, no. 5 (2014): 1715-1722. https://doi.org/10.3892/mmr.2014.2038
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team