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Article

Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice

  • Authors:
    • J. Jin
    • Y. Wang
    • J. Wang
    • Y. Xu
    • S. L. Chen
    • J. P. Wang
    • Y. P. Su
  • View Affiliations / Copyright

    Affiliations: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, P.R. China, Department of Hematology, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China
  • Pages: 1629-1633
    |
    Published online on: March 12, 2014
       https://doi.org/10.3892/mmr.2014.2043
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Abstract

The objective of the present study was to investigate the role of the steroid receptor coactivator‑3 (SRC‑3) in hematopoiesis of mouse bone marrow (BM) following total body irradiation (TBI). SRC‑3‑/‑ mice and wild‑type (WT) mice were exposed to 4.5 Gy γ rays. Immunoblotting analysis revealed that the SRC‑3 protein (p160) levels in normal BM‑nucleated cells in WT were higher than in SRC‑3‑/‑ mice. Furthermore, peripheral blood cell counts, BM cellularity and colony‑forming unit (CFU) assays were performed following irradiation. The results showed that peripheral blood cells were significantly lower in number and recovered less rapidly in irradiated SRC‑3‑/‑ mice as compared with control animals. BM‑nucleated cell and CFU counts were significantly decreased in SRC‑3‑/‑ mice on the 7th and 14th day. Of note, the recovery of platelet (PLT) and megakaryocytic lineage were more depressed than the granulocytic and erythroid lineage in SRC‑3‑/‑ mice. In conclusion, the present study demonstrated that the hematopoietic ability in SRC‑3 knockout mice is severely impaired following a sublethal dose of irradiation.
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Copy and paste a formatted citation
Spandidos Publications style
Jin J, Wang Y, Wang J, Xu Y, Chen SL, Wang JP and Su YP: Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice. Mol Med Rep 9: 1629-1633, 2014.
APA
Jin, J., Wang, Y., Wang, J., Xu, Y., Chen, S.L., Wang, J.P., & Su, Y.P. (2014). Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice. Molecular Medicine Reports, 9, 1629-1633. https://doi.org/10.3892/mmr.2014.2043
MLA
Jin, J., Wang, Y., Wang, J., Xu, Y., Chen, S. L., Wang, J. P., Su, Y. P."Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice". Molecular Medicine Reports 9.5 (2014): 1629-1633.
Chicago
Jin, J., Wang, Y., Wang, J., Xu, Y., Chen, S. L., Wang, J. P., Su, Y. P."Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice". Molecular Medicine Reports 9, no. 5 (2014): 1629-1633. https://doi.org/10.3892/mmr.2014.2043
Copy and paste a formatted citation
x
Spandidos Publications style
Jin J, Wang Y, Wang J, Xu Y, Chen SL, Wang JP and Su YP: Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice. Mol Med Rep 9: 1629-1633, 2014.
APA
Jin, J., Wang, Y., Wang, J., Xu, Y., Chen, S.L., Wang, J.P., & Su, Y.P. (2014). Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice. Molecular Medicine Reports, 9, 1629-1633. https://doi.org/10.3892/mmr.2014.2043
MLA
Jin, J., Wang, Y., Wang, J., Xu, Y., Chen, S. L., Wang, J. P., Su, Y. P."Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice". Molecular Medicine Reports 9.5 (2014): 1629-1633.
Chicago
Jin, J., Wang, Y., Wang, J., Xu, Y., Chen, S. L., Wang, J. P., Su, Y. P."Impaired hematopoiesis and delayed thrombopoietic recovery following sublethal irradiation in SRC‑3 knockout mice". Molecular Medicine Reports 9, no. 5 (2014): 1629-1633. https://doi.org/10.3892/mmr.2014.2043
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