Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis

Hao,Chun‑Qiu; Zhou,Yun; Wang,Jiu‑Ping; Peng,Mei‑Jun; Xie,Yu‑Mei; Kang,Wen‑Zhen; Sun,Li; Wang,Ping‑Zhong; Wan,Chun‑Ling; He,Lin; Cai,Lei; Jia,Zhang‑Sheng

doi:10.3892/mmr.2014.2050

May-2014 Volume 9 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-2014 Volume 9 Issue 5

Full Size Image

Article

Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis

Authors:
- Chun‑Qiu Hao
- Yun Zhou
- Jiu‑Ping Wang
- Mei‑Jun Peng
- Yu‑Mei Xie
- Wen‑Zhen Kang
- Li Sun
- Ping‑Zhong Wang
- Chun‑Ling Wan
- Lin He
- Lei Cai
- Zhang‑Sheng Jia
View Affiliations / Copyright

Affiliations: Bio‑X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiaotong University, Shanghai 200030, P.R. China, Centre of Liver Diseases, Fourth Military Medical University, Tangdu Hospital, Xi'an, Shanxi 710038, P.R. China
Pages: 1743-1748
|
Published online on: March 13, 2014

https://doi.org/10.3892/mmr.2014.2050
Expand metrics +

Abstract

Nogo‑A has been identified as an inhibitor of neurite outgrowth specific to the central nervous system. However, little is known about the role of Nogo‑A in hepatocellular carcinoma (HCC), the most common primary malignant tumor with a high mortality rate. This study aimed to investigate the role of endogenous Nogo‑A in human liver cancer cells. Reverse transcription polymerase chain reaction was used to detect the expression of Nogo‑A in four liver cancer cell lines. A lentivirus vector was then constructed to mediate RNA interference (RNAi) targeting of Nogo‑A (LV‑Nogo‑A‑siRNA) and was confirmed to successfully suppress the expression of the Nogo‑A gene in SMMC‑7721 cells. Furthermore, Nogo‑A was observed to be highly expressed in liver cancer cell lines. RNAi of Nogo‑A using the LV‑Nogo‑A‑siRNA construct significantly decreased Nogo‑A protein expression and specifically inhibited the growth of SMMC‑7721 cells. This growth inhibitory effect may be attributed to an increase in G2/M phase arrest and apoptosis in SMMC‑7721 cells containing Nogo‑A‑siRNA. The results of this study demonstrate that Nogo‑A may represent a novel therapeutic target for the treatment of liver cancer, in addition to its potent roles in neural systems.

View Figures

View References

1	Yang J, Yu L, Bi AD and Zhao SY: Assignment of the human reticulon 4 gene (RTN4) to chromosome 2p14-->2p13 by radiation hybrid mapping. Cytogenet Cell Genet. 88:101–102. 2000. View Article : Google Scholar : PubMed/NCBI
2	GrandPré T, Nakamura F, Vartanian T and Strittmatter SM: Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein. Nature. 403:439–444. 2000.PubMed/NCBI
3	Woolf CJ: No Nogo: now where to go? Neuron. 38:153–156. 2003. View Article : Google Scholar : PubMed/NCBI
4	Chen MS, Huber AB, van der Haar ME, et al: Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature. 403:434–439. 2000. View Article : Google Scholar : PubMed/NCBI
5	Zheng B, Ho C, Li S, Keirstead H, Steward O and Tessier-Lavigne M: Lack of enhanced spinal regeneration in Nogo-deficient mice. Neuron. 38:213–224. 2003. View Article : Google Scholar : PubMed/NCBI
6	Kim JE, Li S, GrandPré T, Qiu D and Strittmatter SM: Axon regeneration in young adult mice lacking Nogo-A/B. Neuron. 38:187–199. 2003. View Article : Google Scholar : PubMed/NCBI
7	Fournier AE, GrandPré T and Strittmatter SM: Identification of a receptor mediating Nogo-66 inhibition of axonal regeneration. Nature. 409:341–346. 2001. View Article : Google Scholar : PubMed/NCBI
8	Xie F and Zheng B: White matter inhibitors in CNS axon regeneration failure. Exp Neurol. 209:302–312. 2008. View Article : Google Scholar : PubMed/NCBI
9	McGee AW, Yang Y, Fischer QS, Daw NW and Strittmatter SM: Experience-driven plasticity of visual cortex limited by myelin and Nogo receptor. Science. 309:2222–2226. 2005. View Article : Google Scholar : PubMed/NCBI
10	Sherman M: Hepatocellular carcinoma: epidemiology, surveillance, and diagnosis. Semin Liver Dis. 30:3–16. 2010. View Article : Google Scholar
11	Novak G and Tallerico T: Nogo A, B and C expression in schizophrenia, depression and bipolar frontal cortex, and correlation of Nogo expression with CAA/TATC polymorphism in 3′-UTR. Brain Res. 1120:161–171. 2006.PubMed/NCBI
12	Lie M, Grover M and Whitlon DS: Accelerated neurite growth from spiral ganglion neurons exposed to the Rho kinase inhibitor H-1152. Neuroscience. 169:855–862. 2010. View Article : Google Scholar : PubMed/NCBI
13	Liu L, Zhang N, Liu J, et al: Lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth. Tumour Biol. 33:515–521. 2012. View Article : Google Scholar : PubMed/NCBI
14	Chen YC, Lu DD, Cao XR and Zhang XR: RTN4-C gene expression in hepatocellular carcinoma and its influence on SMMC7721 cell growth and apoptosis. Yi Chuan Xue Bao. 32:891–897. 2005.PubMed/NCBI
15	Kuang E, Wan Q, Li X, Xu H, Zou T and Qi Y: ER stress triggers apoptosis induced by Nogo-B/ASY overexpression. Exp Cell Res. 312:1983–1988. 2006. View Article : Google Scholar : PubMed/NCBI
16	Zheng H, Xue S, Lian F and Wang YY: A novel promising therapy for vein graft restenosis: overexpressed Nogo-B induces vascular smooth muscle cell apoptosis by activation of the JNK/p38 MAPK signaling pathway. Med Hypotheses. 77:278–281. 2011. View Article : Google Scholar : PubMed/NCBI
17	Naldini L, Blömer U, Gallay P, et al: In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science. 272:263–267. 1996. View Article : Google Scholar : PubMed/NCBI
18	Brummelkamp TR, Bernards R and Agami R: A system for stable expression of short interfering RNAs in mammalian cells. Science. 296:550–553. 2002. View Article : Google Scholar : PubMed/NCBI
19	Rubinson DA, Dillon CP, Kwiatkowski AV, et al: A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet. 33:401–406. 2003. View Article : Google Scholar : PubMed/NCBI
20	Sutendra G, Dromparis P, Wright P, et al: The role of Nogo and the mitochondria-endoplasmic reticulum unit in pulmonary hypertension. Sci Transl Med. 3:88ra552011.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles