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Article

Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering

  • Authors:
    • Cancan Cheng
    • Yanyan Zhou
    • Biao Kan
    • Qian Wang
    • Yongyu Rui
  • View Affiliations / Copyright

    Affiliations: Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, The Priority Laboratory of Medical Molecular Bacteriology of the Ministry of Health, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China
  • Pages: 2239-2244
    |
    Published online on: March 24, 2014
       https://doi.org/10.3892/mmr.2014.2065
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Abstract

The present study aimed to construct and evaluate the live attenuated Vibrio cholerae serogroup O139 vaccine candidate, in which genes encoding protective antigens were integrated into the chromosomal DNA. Using the initial strain, O139‑ZJ9693, the toxin‑linked cryptic (TLC) and cholera toxin (CTX) genetic elements and repeats in the toxin (RTX) gene cluster were deleted from its chromosomal DNA, and the cholera toxin genes, ctxB and rstR, were transferred into the chromosome to construct the candidate vaccine strain. The expression of ctxB and the vaccine virulence were then examined. Polymerase chain reaction (PCR), enzymatic digestion and electrophoresis were performed to confirm that TLC, CTX and RTX were deleted, and that ctxB and rstR were transferred into the vaccine candidate DNA. According to the preliminary evaluation, the ctxB gene exhibited cholera toxin subunit B expression, and no enterotoxigenic or cytotoxic effects were observed in this strain. In conclusion, a recombinant strain containing genes encoding protective antigens that replaced virulence‑associated genes was successfully constructed in the present study; this candidate strain may have the potential to be utilized to further evaluate the immune response.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng C, Zhou Y, Kan B, Wang Q and Rui Y: Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering. Mol Med Rep 9: 2239-2244, 2014.
APA
Cheng, C., Zhou, Y., Kan, B., Wang, Q., & Rui, Y. (2014). Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering. Molecular Medicine Reports, 9, 2239-2244. https://doi.org/10.3892/mmr.2014.2065
MLA
Cheng, C., Zhou, Y., Kan, B., Wang, Q., Rui, Y."Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering". Molecular Medicine Reports 9.6 (2014): 2239-2244.
Chicago
Cheng, C., Zhou, Y., Kan, B., Wang, Q., Rui, Y."Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering". Molecular Medicine Reports 9, no. 6 (2014): 2239-2244. https://doi.org/10.3892/mmr.2014.2065
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng C, Zhou Y, Kan B, Wang Q and Rui Y: Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering. Mol Med Rep 9: 2239-2244, 2014.
APA
Cheng, C., Zhou, Y., Kan, B., Wang, Q., & Rui, Y. (2014). Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering. Molecular Medicine Reports, 9, 2239-2244. https://doi.org/10.3892/mmr.2014.2065
MLA
Cheng, C., Zhou, Y., Kan, B., Wang, Q., Rui, Y."Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering". Molecular Medicine Reports 9.6 (2014): 2239-2244.
Chicago
Cheng, C., Zhou, Y., Kan, B., Wang, Q., Rui, Y."Construction and characterization of a Vibrio cholerae serogroup O139 vaccine candidate by genetic engineering". Molecular Medicine Reports 9, no. 6 (2014): 2239-2244. https://doi.org/10.3892/mmr.2014.2065
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