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Article

Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells

  • Authors:
    • Fumin Wang
    • Yucheng Lu
    • Faying Qi
    • Quanping Su
    • Long  Wang
    • Cuiping You
    • Fengyuan Che
    • Jixu Yu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Neurology, School of Neurology, Weifang Medical University, Weifang, Shandong 261053, P.R. China, Central Laboratory, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China, Department of Neurology, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China
  • Pages: 2453-2458
    |
    Published online on: March 28, 2014
       https://doi.org/10.3892/mmr.2014.2087
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Abstract

Dominant mutations in superoxide dismutase 1 (SOD1) are a frequent cause of the lethal neurodegenerative disease amyotrophic lateral sclerosis (ALS). The nuclear factor erythroid 2‑related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway is the major cellular defense mechanism against oxidative stress, however, its role in ALS remains to be fully elucidated. Therefore, the present study aimed to investigate whether the human SOD1‑G93A gene affected the Nrf2/ARE signaling pathway in an ALS cell model. The soma became round and the number of neurites decreased in the NSC‑34 cells transfected with the hSOD1‑G93A gene, and the neurites were shorter and oxidative stress was increased compared with the normal NSC‑34 cells. Furthermore, the mRNA and protein expression of Nrf2, heme oxygenase‑1 and NAD(P)H: quinone oxidoreductase 1 was significantly decreased in the NSC‑34 cells transfected with the human SOD1‑G93A gene. The present study indicated that human SOD1‑G93A damaged the Nrf2/ARE signaling pathway in the ALS cell model and reduced the ability of cells to protect against oxidative injury.
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Copy and paste a formatted citation
Spandidos Publications style
Wang F, Lu Y, Qi F, Su Q, Wang L, You C, Che F and Yu J: Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells. Mol Med Rep 9: 2453-2458, 2014.
APA
Wang, F., Lu, Y., Qi, F., Su, Q., Wang, L., You, C. ... Yu, J. (2014). Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells. Molecular Medicine Reports, 9, 2453-2458. https://doi.org/10.3892/mmr.2014.2087
MLA
Wang, F., Lu, Y., Qi, F., Su, Q., Wang, L., You, C., Che, F., Yu, J."Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells". Molecular Medicine Reports 9.6 (2014): 2453-2458.
Chicago
Wang, F., Lu, Y., Qi, F., Su, Q., Wang, L., You, C., Che, F., Yu, J."Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells". Molecular Medicine Reports 9, no. 6 (2014): 2453-2458. https://doi.org/10.3892/mmr.2014.2087
Copy and paste a formatted citation
x
Spandidos Publications style
Wang F, Lu Y, Qi F, Su Q, Wang L, You C, Che F and Yu J: Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells. Mol Med Rep 9: 2453-2458, 2014.
APA
Wang, F., Lu, Y., Qi, F., Su, Q., Wang, L., You, C. ... Yu, J. (2014). Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells. Molecular Medicine Reports, 9, 2453-2458. https://doi.org/10.3892/mmr.2014.2087
MLA
Wang, F., Lu, Y., Qi, F., Su, Q., Wang, L., You, C., Che, F., Yu, J."Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells". Molecular Medicine Reports 9.6 (2014): 2453-2458.
Chicago
Wang, F., Lu, Y., Qi, F., Su, Q., Wang, L., You, C., Che, F., Yu, J."Effect of the human SOD1‑G93A gene on the Nrf2/ARE signaling pathway in NSC‑34 cells". Molecular Medicine Reports 9, no. 6 (2014): 2453-2458. https://doi.org/10.3892/mmr.2014.2087
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