Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
June-2014 Volume 9 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2014 Volume 9 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells

  • Authors:
    • Bu‑Shan Xie
    • Xing‑Xing He
    • Zheng‑Lin Ai
    • Shu‑Kun Yao
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China, Graduate School, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, P.R. China, Department of Gastroenterology, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
  • Pages: 2547-2553
    |
    Published online on: April 2, 2014
       https://doi.org/10.3892/mmr.2014.2107
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Matrine, one of the main components extracted from Sophora flavescens, has exhibited pharmacological effects on the differentiation in rat liver oval cells. However, its function and mechanism have not yet been fully elucidated. To further investigate them, an in vitro model was established using a rat liver oval cell line called WB‑F344 and treated with matrine. Initially, a significant increase in the number of monodansylcadaverine‑positive cells and in the levels of microtubule‑associated protein 1A/1B‑light chain 3 (LC3)‑II, which is a specific marker for detecting autophagy, was observed in matrine‑treated cells. This indicated that autophagy was stimulated by matrine, which was further confirmed by transmission electron microscopy. Additionally, the apoptotic oval cells were easily detected under matrine treatment using an Annexin‑V‑fluorescein isothiocyanate/propidium iodide assay, indicating that autophagy and apoptosis were synchronously induced by matrine. A decrease in B‑cell lymphoma (Bcl‑2) mRNA expression, but an increase in Bcl2‑associated X protein (Bax) mRNA expression were observed in matrine‑treated cells, which led to an upregulation of the Bax/Bcl‑2 ratio, a molecular marker for determining the extent of apoptosis. Next, the molecular mechanism of matrine‑induced autophagy and apoptosis was analyzed in WB‑F344 cells. β‑catenin degradation was downregulated by matrine and rapamycin, a foregone chemical agonist of autophagy, whereas it was upregulated by 3‑methyladenine, a specific inhibitor of autophagy. Additionally, β‑catenin activation induced an increase in LC3‑II levels and reversed the Bax/Bcl‑2 mRNA ratio under matrine treatment, whereas inhibition of β‑catenin by RNA interference induced a decrease of the LC3‑II amount and of the Bax/Bcl‑2 mRNA ratio. Finally, matrine treatment attenuated p53; however, with little or no change in LC3‑II levels, but a decrease in β‑catenin levels occurred in WB‑F344 cells upon treatment with pifithrin‑α, a chemical inhibitor of p53, revealing that p53, interfering with β‑catenin, may not be involved in matrine‑induced autophagy in WB‑F344 cells. These results demonstrate that β‑catenin is involved in matrine‑induced autophagy and apoptosis in WB‑F344 cells, while β‑catenin is negatively regulated by autophagy and positively by p53, indicating that β‑catenin may be involved in the crosstalk between autophagy and apoptosis in WB‑F344 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Luo C, Zhu Y, Jiang T, et al: Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family. Toxicology. 229:245–252. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Long Y, Lin XT, Zeng KL and Zhang L: Efficacy of intramuscular matrine in the treatment of chronic hepatitis B. Hepatobiliary Pancreat Dis Int. 3:69–72. 2004.PubMed/NCBI

3 

Guo D, Chen NN, Zhou P, Pan B and Hou LB: Suppressive effect of matrine on cell growth and decreases beta-catenin-dependent transcriptional activity in hepatoma cell line Hep3B. Journal of Chinese Medicinal Materials. 33:778–781. 2010.(In Chinese).

4 

Zhang Y, Zhang H, Yu P, et al: Effects of matrine against the growth of human lung cancer and hepatoma cells as well as lung cancer cell migration. Cytotechnology. 59:191–200. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Zhang LP, Jiang JK, Tam JW, et al: Effects of Matrine on proliferation and differentiation in K-562 cells. Leuk Res. 25:793–800. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Zhang S, Qi J, Sun L, et al: Matrine induces programmed cell death and regulates expression of relevant genes based on PCR array analysis in C6 glioma cells. Mol Biol Rep. 36:791–799. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Coleman WB, Wennerberg AE, Smith GJ and Grisham JW: Regulation of the differentiation of diploid and some aneuploid rat liver epithelial (stemlike) cells by the hepatic microenvironment. Am J Pathol. 142:1373–1382. 1993.PubMed/NCBI

8 

Coleman WB, Smith GJ and Grisham JW: Development of dexamethasone-inducible tyrosine aminotransferase activity in WB-F344 rat liver epithelial stemlike cells cultured in the presence of sodium butyrate. J Cell Physiol. 161:463–469. 1994. View Article : Google Scholar

9 

Couchie D, Holic N, Chobert MN, Corlu A and Laperche Y: In vitro differentiation of WB-F344 rat liver epithelial cells into the biliary lineage. Differentiation. 69:209–215. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Tsao MS and Grisham JW: Hepatocarcinomas, cholangiocarcinomas, and hepatoblastomas produced by chemically transformed cultured rat liver epithelial cells. A light- and electron-microscopic analysis. Am J Pathol. 127:168–181. 1987.

11 

Biederbick A, Kern HF and Elsasser HP: Monodansylcadaverine (MDC) is a specific in vivo marker for autophagic vacuoles. Eur J Cell Biol. 66:3–14. 1995.PubMed/NCBI

12 

Kabeya Y, Mizushima N, Ueno T, et al: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19:5720–5728. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Caro LH, Plomp PJ, Wolvetang EJ, Kerkhof C and Meijer AJ: 3-Methyladenine, an inhibitor of autophagy, has multiple effects on metabolism. Eur J Biochem. 175:325–329. 1988. View Article : Google Scholar : PubMed/NCBI

14 

Krajarng A, Nakamura Y, Suksamrarn S and Watanapokasin R: alpha-Mangostin induces apoptosis in human chondrosarcoma cells through downregulation of ERK/JNK and Akt signaling pathway. J Agric Food Chem. 59:5746–5754. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Nakamura T, Sano M, Songyang Z and Schneider MD: A Wnt- and beta-catenin-dependent pathway for mammalian cardiac myogenesis. Proc Natl Acad Sci USA. 100:5834–5839. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Bjornsti MA and Houghton PJ: The TOR pathway: a target for cancer therapy. Nat Rev Cancer. 4:335–348. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Levine B and Abrams J: p53: The Janus of autophagy? Nat Cell Biol. 10:637–639. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Bassi L, Carloni M, Fonti E, Palma de la Pena N, Meschini R and Palitti F: Pifithrin-alpha, an inhibitor of p53, enhances the genetic instability induced by etoposide (VP16) in human lymphoblastoid cells treated in vitro. Mutat Res. 499:163–176. 2002. View Article : Google Scholar

19 

Lee SB, Tong SY, Kim JJ, Um SJ and Park JS: Caspase-independent autophagic cytotoxicity in etoposide-treated CaSki cervical carcinoma cells. DNA Cell Biol. 26:713–720. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Harhaji-Trajkovic L, Vilimanovich U, Kravic-Stevovic T, Bumbasirevic V and Trajkovic V: AMPK-mediated autophagy inhibits apoptosis in cisplatin-treated tumor cells. J Cell Mol Med. 13:3644–3654. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Nishikawa T, Tsuno NH, Okaji Y, et al: Inhibition of autophagy potentiates sulforaphane-induced apoptosis in human colon cancer cells. Ann Surg Oncol. 17:592–602. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Abedin MJ, Wang D, McDonnell MA, Lehmann U and Kelekar A: Autophagy delays apoptotic death in breast cancer cells following DNA damage. Cell Death Differ. 14:500–510. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Xie BS, Zhao HC, Yao SK, et al: Autophagy inhibition enhances etoposide-induced cell death in human hepatoma G2 cells. Int J Mol Med. 27:599–606. 2011.PubMed/NCBI

24 

Guo L, Xie B and Mao Z: Autophagy in premature senescent cells is activated via AMPK pathway. Int J Mol Sci. 13:3563–3582. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Kato H, Gruenwald A, Suh JH, et al: The Wnt/{beta}-catenin pathway in podocytes integrates cell adhesion, differentiation and survival. J Biol Chem. 286:26003–26015. 2011.

26 

Thompson MD and Monga SP: WNT/beta-catenin signaling in liver health and disease. Hepatology. 45:1298–1305. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Moon RT, Bowerman B, Boutros M and Perrimon N: The promise and perils of Wnt signaling through beta-catenin. Science. 296:1644–1646. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Gao C, Cao W, Bao L, et al: Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation. Nat Cell Biol. 12:781–790. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Maiuri MC, Zalckvar E, Kimchi A and Kroemer G: Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol. 8:741–752. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Li M, Jiang X, Liu D, Na Y, Gao GF and Xi Z: Autophagy protects LNCaP cells under androgen deprivation conditions. Autophagy. 4:54–60. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Pattingre S, Tassa A, Qu X, et al: Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell. 122:927–939. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Sadot E, Geiger B, Oren M and Ben-Ze’ev A: Down-regulation of beta-catenin by activated p53. Mol Cell Biol. 21:6768–6781. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xie BS, He XX, Ai ZL and Yao SK: Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells. Mol Med Rep 9: 2547-2553, 2014.
APA
Xie, B., He, X., Ai, Z., & Yao, S. (2014). Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells. Molecular Medicine Reports, 9, 2547-2553. https://doi.org/10.3892/mmr.2014.2107
MLA
Xie, B., He, X., Ai, Z., Yao, S."Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells". Molecular Medicine Reports 9.6 (2014): 2547-2553.
Chicago
Xie, B., He, X., Ai, Z., Yao, S."Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells". Molecular Medicine Reports 9, no. 6 (2014): 2547-2553. https://doi.org/10.3892/mmr.2014.2107
Copy and paste a formatted citation
x
Spandidos Publications style
Xie BS, He XX, Ai ZL and Yao SK: Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells. Mol Med Rep 9: 2547-2553, 2014.
APA
Xie, B., He, X., Ai, Z., & Yao, S. (2014). Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells. Molecular Medicine Reports, 9, 2547-2553. https://doi.org/10.3892/mmr.2014.2107
MLA
Xie, B., He, X., Ai, Z., Yao, S."Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells". Molecular Medicine Reports 9.6 (2014): 2547-2553.
Chicago
Xie, B., He, X., Ai, Z., Yao, S."Involvement of β‑catenin in matrine‑induced autophagy and apoptosis in WB‑F344 cells". Molecular Medicine Reports 9, no. 6 (2014): 2547-2553. https://doi.org/10.3892/mmr.2014.2107
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team