Invadopodia are essential in transurothelial invasion during the muscle invasion of bladder cancer cells

Imanishi,Kengo; Yoneyama,Mihoko  Sutoh; Hatakeyama,Shingo; Yamamoto,Hayato; Koie,Takuya; Saitoh,Hisao; Yamaya,Kanemitsu; Funyu,Tomihisa; Nakamura,Toshiya; Ohyama,Chikara; Tsuboi,Shigeru

doi:10.3892/mmr.2014.2113

June-2014 Volume 9 Issue 6

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June-2014 Volume 9 Issue 6

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Article

Invadopodia are essential in transurothelial invasion during the muscle invasion of bladder cancer cells

Authors:
- Kengo Imanishi
- Mihoko Sutoh Yoneyama
- Shingo Hatakeyama
- Hayato Yamamoto
- Takuya Koie
- Hisao Saitoh
- Kanemitsu Yamaya
- Tomihisa Funyu
- Toshiya Nakamura
- Chikara Ohyama
- Shigeru Tsuboi
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Affiliations: Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036‑8562, Japan, Urology, Oyokyo Kidney Research Institute, Hirosaki, Aomori 036‑8243, Japan, Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan
Pages: 2159-2165
|
Published online on: April 3, 2014

https://doi.org/10.3892/mmr.2014.2113
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Abstract

Muscle invasive bladder cancer is an aggressive type of epithelial tumor with a high rate of metastasis. For bladder cancer cells to reach the muscle layer, cells must invade through an urothelial cell monolayer (transurothelial invasion) and basement membrane. However, the process by which transurothelial invasion occurs has not been fully characterized. In this study we developed a novel method to evaluate the transurothelial invasion capacity and investigated its cellular and molecular processes using primary culture cells from bladder cancer patients. The analysis revealed that compared with the prognosis for patients with non‑muscle invasive bladder cancer that of patients with muscle invasive bladder cancer was particularly poor due to metastatic recurrence. Cancer cells from patients with muscle invasive bladder cancer exhibited a higher invasive capacity through the urothelial cell monolayer compared with those from non‑invasive bladder cancer patients. Furthermore, muscle invasive bladder cancer cells demonstrated a greater ability to form invadopodia, the filamentous actin‑based membrane protrusions required for matrix degradation and invasion compared with non‑invasive cells. Bladder cancer cell lines were established with reduced invadopodia formation by silencing the expression of cortactin, an essential component of invadopodia. The cortactin knockdown bladder cancer cells with reduced invadopodia formation demonstrated a markedly reduced ability to invade through the urothelial cell monolayer, indicating that invadopodia are essential for transurothelial invasion. The results indicate that invadopodia formation is required for muscle invasion of aggressive bladder cancer cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Invadopodia are essential in transurothelial invasion during the muscle invasion of bladder cancer cells

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