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Article

Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells

  • Authors:
    • Xiujuan Zhang
    • Wenxia Han
    • Xiuyun Jiang
    • Min Li
    • Ling Gao
    • Jia Jun Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, The Scientific Center, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
  • Pages: 2554-2558
    |
    Published online on: April 8, 2014
       https://doi.org/10.3892/mmr.2014.2122
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Abstract

Previous studies have demonstrated that sustained high leucine exposure decreases glucose‑stimulated insulin secretion (GSIS). However, whether this effect is recoverable following the removal of leucine is unclear. Pancreatic/duodenal homeobox‑1 (PDX‑1) and its downstream target, glucose transporter 2 (GLUT2), are reported to be positively associated with insulin secretion. However, it also remains unclear whether the effect of leucine on GSIS is accompanied by alterations in PDX‑1 and GLUT2. In the present study, insulin secretion, insulin content, PDX‑1 and GLUT2 protein expression in INS‑1 (rat insulinoma cell line) cells were assessed following a 24‑h incubation in 40 mmol/l leucine. Half of the cells were incubated in leucine‑free media for a further 24 h to observe the abovementioned effects. In contrast to the control, 40 mmol/l leucine for 24 or 48 h diminished GSIS at high glucose concentrations by 11% (P=0.026) or 22% (P=0.003), insulin content by 14% (P=0.008) or 20% (P=0.002), as well as decreasing PDX‑1 and GLUT2 expression. When leucine was removed from the media for a further 24‑h incubation, in comparison with those cells that were maintained in leucine treatment for 24 and 48 h, the high GSIS increased by 13% (P=0.032) and 27% (P=0.002), insulin content was augmented by 10% (P=0.014) and 20% (P=0.003), and the protein expression of PDX‑1 and GLUT2 also increased. The present study demonstrates that sustained high concentrations of leucine induce a reversible impairment of GSIS and alter insulin content, which is mediated by PDX‑1 and GLUT2, in INS‑1 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Han W, Jiang X, Li M, Gao L and Zhao JJ: Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells. Mol Med Rep 9: 2554-2558, 2014.
APA
Zhang, X., Han, W., Jiang, X., Li, M., Gao, L., & Zhao, J.J. (2014). Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells. Molecular Medicine Reports, 9, 2554-2558. https://doi.org/10.3892/mmr.2014.2122
MLA
Zhang, X., Han, W., Jiang, X., Li, M., Gao, L., Zhao, J. J."Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells". Molecular Medicine Reports 9.6 (2014): 2554-2558.
Chicago
Zhang, X., Han, W., Jiang, X., Li, M., Gao, L., Zhao, J. J."Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells". Molecular Medicine Reports 9, no. 6 (2014): 2554-2558. https://doi.org/10.3892/mmr.2014.2122
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Han W, Jiang X, Li M, Gao L and Zhao JJ: Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells. Mol Med Rep 9: 2554-2558, 2014.
APA
Zhang, X., Han, W., Jiang, X., Li, M., Gao, L., & Zhao, J.J. (2014). Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells. Molecular Medicine Reports, 9, 2554-2558. https://doi.org/10.3892/mmr.2014.2122
MLA
Zhang, X., Han, W., Jiang, X., Li, M., Gao, L., Zhao, J. J."Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells". Molecular Medicine Reports 9.6 (2014): 2554-2558.
Chicago
Zhang, X., Han, W., Jiang, X., Li, M., Gao, L., Zhao, J. J."Chronic leucine exposure results in reduced but reversible glucose-stimulated insulin secretion in INS-1 cells". Molecular Medicine Reports 9, no. 6 (2014): 2554-2558. https://doi.org/10.3892/mmr.2014.2122
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