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Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice

  • Authors:
    • Zhen‑Yi Jia
    • Yang Xia
    • Danian Tong
    • Jing Yao
    • Hong‑Qi Chen
    • Jun Yang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2205-2212
    |
    Published online on: April 9, 2014
       https://doi.org/10.3892/mmr.2014.2137
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Abstract

Complex communities of microorganisms play important roles in human health, and alterations in the intestinal microbiota may induce intestinal inflammation and numerous diseases. The purpose of this study was to identify the key genes and processes affected by depletion of the intestinal microbiota in a murine model. The Affymetrix microarray dataset GSE22648 was downloaded from the Gene Expression Omnibus database, and differentially expressed genes (DEGs) were identified using the limma package in R. A protein‑protein interaction (PPI) network was constructed for the DEGs using the Cytoscape software, and the network was divided into several modules using the MCODE plugin. Furthermore, the modules were functionally annotated using the PiNGO plugin, and DEG‑related pathways were retrieved and analyzed using the GenMAPP software. A total of 53 DEGs were identified, of which 26 were upregulated and 27 were downregulated. The PPI network of these DEGs comprised 3 modules. The most significant module‑related DEGs were the cytochrome P450 (CYP) 4B1 isozyme gene (CYP4B1) in module 1, CYP4F14 in module 2 and the tachykinin precursor 1 gene (TAC1) in module 3. The majority of enriched pathways of module 1 and 2 were oxidation reduction pathways (metabolism of xenobiotics by CYPs) and lipid metabolism‑related pathways, including linoleic acid and arachidonic acid metabolism. The neuropeptide signaling pathway was the most significantly enriched functional pathway of module 3. In conclusion, our findings strongly suggest that intestinal microbiota depletion affects cellular metabolism and oxidation reduction pathways. In addition, this is the first time, to the best of our knowledge, that the neuropeptide signaling pathway is reported to be affected by intestinal microbiota depletion in mice. The present study provides a list of candidate genes and processes related to the interaction of microbiota with the intestinal tract.
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Copy and paste a formatted citation
Spandidos Publications style
Jia ZY, Xia Y, Tong D, Yao J, Chen HQ and Yang J: Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice. Mol Med Rep 9: 2205-2212, 2014.
APA
Jia, Z., Xia, Y., Tong, D., Yao, J., Chen, H., & Yang, J. (2014). Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice. Molecular Medicine Reports, 9, 2205-2212. https://doi.org/10.3892/mmr.2014.2137
MLA
Jia, Z., Xia, Y., Tong, D., Yao, J., Chen, H., Yang, J."Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice". Molecular Medicine Reports 9.6 (2014): 2205-2212.
Chicago
Jia, Z., Xia, Y., Tong, D., Yao, J., Chen, H., Yang, J."Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice". Molecular Medicine Reports 9, no. 6 (2014): 2205-2212. https://doi.org/10.3892/mmr.2014.2137
Copy and paste a formatted citation
x
Spandidos Publications style
Jia ZY, Xia Y, Tong D, Yao J, Chen HQ and Yang J: Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice. Mol Med Rep 9: 2205-2212, 2014.
APA
Jia, Z., Xia, Y., Tong, D., Yao, J., Chen, H., & Yang, J. (2014). Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice. Molecular Medicine Reports, 9, 2205-2212. https://doi.org/10.3892/mmr.2014.2137
MLA
Jia, Z., Xia, Y., Tong, D., Yao, J., Chen, H., Yang, J."Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice". Molecular Medicine Reports 9.6 (2014): 2205-2212.
Chicago
Jia, Z., Xia, Y., Tong, D., Yao, J., Chen, H., Yang, J."Module-based functional pathway enrichment analysis of a protein‑protein interaction network to study the effects of intestinal microbiota depletion in mice". Molecular Medicine Reports 9, no. 6 (2014): 2205-2212. https://doi.org/10.3892/mmr.2014.2137
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