Application of two-dimensional electrophoresis and mass spectrometry to screen endometriosis‑related proteins

Tuerxun,Hanikezi; Zhang,Yanmei; Ji,Fei; Aili,Aixingzi; Yang,Xinhua; Ding,Yan

doi:10.3892/mmr.2014.2142

July-2014 Volume 10 Issue 1

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July-2014 Volume 10 Issue 1

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Article

Application of two-dimensional electrophoresis and mass spectrometry to screen endometriosis‑related proteins

Authors:
- Hanikezi Tuerxun
- Yanmei Zhang
- Fei Ji
- Aixingzi Aili
- Xinhua Yang
- Yan Ding
View Affiliations / Copyright

Affiliations: Gynecology Department, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China, Department of Obstetrics, Xinjiang Uygur Autonomous Region People's Hospital, Urumqi, Xinjiang 830001, P.R. China, Department of Gynecology, Shanghai Dongfang Hospital, Shanghai 200120, P.R. China
Pages: 95-100
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Published online on: April 15, 2014

https://doi.org/10.3892/mmr.2014.2142
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Abstract

The aim of the present study was to detect the differences in the protein expression between Uyghur females with or without endometriosis (EM). The two dimensional (2D) gel images of proteins extracted from the serum of Uyghur and Han females with EM and the controls were analyzed using Phoretix 2D software, and the differentially expressed proteins were identified primarily by database query. Having compared the reproducible 2D gel images of proteins from the serum of Uihgur and Han females with and without EM, 13 differentially expressed proteins were obtained from Uyghur females and eight differentially expressed proteins were obtained from Han females. The present study demonstrated ethnic differences in gene and protein expression between Uyghur and Han females with EM.

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1	Valle RF and Sciarra JJ: Endometriosis: treatment strategies. Ann NY Acad Sci. 997:229–239. 2003. View Article : Google Scholar : PubMed/NCBI
2	Dunn SR and Pennington MJ: Proteomics: From Protein Sequence to Function. BIOS Scientific Publishers; Oxford, UK: pp. 122–134. 2001
3	Rout MP, Aitchison JD, Suprapto A, Hjertaas K, Zhao Y and Chait BT: The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol. 148:635–651. 2000. View Article : Google Scholar : PubMed/NCBI
4	Newman JR, Wolf E and Kim PS: A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae. Proc Natl Acad Sci USA. 97:13203–13208. 2000. View Article : Google Scholar : PubMed/NCBI
5	Wall DB, Kachman MT, Gong SS, Parus SJ, Long MW and Lubman DM: Isoelectric focusing nonporous silica reversed-phase high-performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry: a three-dimensional liquid-phase protein separation method as applied to the human erythroleukemia cell-line. Rapid Commun Mass Spectrom. 15:1649–1661. 2001.
6	Wang H and Hanash S: Multi-dimensional liquid phase based separations in proteomics. J Chromatogr B Analyt Technol Biomed Life Sci. 787:11–18. 2003. View Article : Google Scholar : PubMed/NCBI
7	Nisolle M, Casanas-Roux F and Donnez J: Immunohistochemical analysis of proliferative activity and steroid receptor expression in peritoneal and ovarian endometriosis. Fertil Steril. 68:912–919. 1997. View Article : Google Scholar : PubMed/NCBI
8	Küster B, Mortensen P, Andersen JS and Mann M: Mass spectrometry allows direct indetification of protein in large genomes. Protemics. 1:641–650. 2001.
9	Demir AY, Groothuis PG, Nap AW, Punyadeera C, de Goeij AF, Evers JL and Dunselman GA: Menstrual effluent induces epithelial-mesenchymal transitions in mesothelial cells. Hum Reprod. 19:21–29. 2004. View Article : Google Scholar : PubMed/NCBI
10	Miettinen M and Fetsch JF: Distribution of keratins in normal endothelial cells and a spectrum of vascular tumors: implications in tumor diagnosis. Hum Pathol. 31:1062–1067. 2000. View Article : Google Scholar : PubMed/NCBI
11	Gao YH, Qi NL, Zhang P, et al: A novel human gene spindlin 1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell’s transformation. Prog Nat Sci. 14:1058–1062. 2004.(In Chinese).
12	Cody CW, Prasher DC, Westler WM, Prendergast FG and Ward WW: Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry. 32:1212–1218. 1993. View Article : Google Scholar : PubMed/NCBI
13	Clup P, Nüsslein-Volhard C and Hopkins N: High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proc Natl Acad Sci USA. 88:7953–7957. 1991. View Article : Google Scholar : PubMed/NCBI
14	Fainaru O, Adini A, Benny O, et al: Dendritic cells support angiogenesis and promote lesion growth in a murine model of endometriosis. FASEB J. 22:522–529. 2008. View Article : Google Scholar : PubMed/NCBI
15	Braun DP, Ding J, Shen J, Rana N, Fernandez B and Dmowski WP: Relationship between apoptosis and the number of macrophages in eutopic endometrium from women with and without endometriosis. Fertil Steril. 78:830–835. 2002. View Article : Google Scholar : PubMed/NCBI
16	Sadeghi H and Taylor HS: HOXA10 regulates endometrial GABAA π receptor expression and membrane translocation. Am J Physiol Endocrinol Metab. 298:E889–E893. 2010.PubMed/NCBI
17	Ametzazurra A, Matorras R, García-Velasco JA, et al: Endometrial fluid is a specific and non-invasive biological sample for protein biomarker identification in endometriosis. Hum Reprod. 24:954–965. 2009. View Article : Google Scholar : PubMed/NCBI
18	Zhang H, Niu Y, Feng J, Guo H, Ye X and Cui H: Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls. Fertil Steril. 86:274–282. 2006. View Article : Google Scholar : PubMed/NCBI