Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion

Kageyama,Yasunari; Doi,Tomoaki; Matsushima‑Nishiwaki,Rie; Iida,Yuko; Akamatsu,Shigeru; Kondo,Akira; Kuroyanagi,Gen; Yamamoto,Naohiro; Mizutani,Jun; Otsuka,Takanobu; Tokuda,Haruhiko; Iida,Hiroki; Kozawa,Osamu; Ogura,Shinji

doi:10.3892/mmr.2014.2143

July-2014 Volume 10 Issue 1

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July-2014 Volume 10 Issue 1

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Article

Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion

Authors:
- Yasunari Kageyama
- Tomoaki Doi
- Rie Matsushima‑Nishiwaki
- Yuko Iida
- Shigeru Akamatsu
- Akira Kondo
- Gen Kuroyanagi
- Naohiro Yamamoto
- Jun Mizutani
- Takanobu Otsuka
- Haruhiko Tokuda
- Hiroki Iida
- Osamu Kozawa
- Shinji Ogura
View Affiliations / Copyright

Affiliations: Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan, Department of Intensive Care Medicine, Matsunami General Hospital, Gifu 501‑6062, Japan, Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467‑8601, Japan, Department of Anesthesiology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan
Pages: 107-112
|
Published online on: April 15, 2014

https://doi.org/10.3892/mmr.2014.2143
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Abstract

We have previously shown that glycoprotein Ib/IX/V activation stimulates the release of the soluble CD40 ligand (sCD40L) via the generation of thromboxane A2 from human platelets. In the present study, the role of Rac, which is a member of the Rho family, was investigated in the thromboxane A2‑stimulated release of platelet‑derived growth factor (PDGF)‑AB and sCD40L in human platelets. U46619, a thromboxane receptor agonist, stimulated the activation of Rac time‑dependently in human platelets, and NSC23766, a selective inhibitor of the Rac‑guanine nucleotide exchange factor interaction, reduced the U46619‑induced platelet aggregation. NSC23766 markedly suppressed the U46619‑induced p38 mitogen-activated protein (MAP) kinase phosphorylation. The thromboxane A2‑induced release of PDGF‑AB and sCD40L was significantly suppressed by NSC23766 in a dose‑dependent manner. In addition, NSC23766 reduced the sCD40L release stimulated by ristocetin, a glycoprotein Ib/IX/V activator. These results indicate that Rac regulates the thromboxane A2‑induced stimulation of PDGF‑AB secretion and sCD40L release via the p38 MAP kinase in human platelets.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion

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