Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2014 Volume 10 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2014 Volume 10 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function

  • Authors:
    • Cheng Diao
    • Zanmei Qi
    • Shuyan Du
    • Xinzhe Cai
    • Yi Jiang
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, The First Affiliated Hospital, China Medical University, Shengyang, Liaoning 110001, P.R. China, Department of Immunology, College of Basic Medical Sciences, China Medical University, Shengyang, Liaoning 110001, P.R. China, Central Laboratory and Department of Dermatology, The First Affiliated Hospital, China Medical University, Shengyang, Liaoning 110001, P.R. China
  • Pages: 353-358
    |
    Published online on: April 15, 2014
       https://doi.org/10.3892/mmr.2014.2147
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the signal transduction mechanism of the NZW type interleukin (IL)‑10R1 and the effect on the function of B lymphocytes. Vectors for the expression of wild‑type, NZW and G1146A IL‑10R1 were constructed and transfected into Ba/F3 cells. The cell proliferation curve was determined using an MTT assay. CD32 and leukocyte-endothelial cell adhesion molecule 1 (LECAM‑1) expression levels were assessed using flow cytometry. The phosphorylation of Janus kinase 1 (Jak1), tyrosine kinase 2 (Tyk2) and signal transducer and activator of transcription 3 (Stat3) were measured by western blot analysis. As compared with wild‑type IL‑10R1 cells, the proliferation ability of NZW IL‑10R1 cells was increased following stimulation with IL‑10, which was even greater for G1146A cells. CD32 levels in NZW IL‑10R1 cells were lower, while the expression of LECAM‑1 was higher compared with wild‑type IL‑10R1 cells, which was also observed for G1146A cells. The phosphorylation levels of Jak1 and Stat3 in NZW IL‑10R1 and G1146A IL‑10R1 cells were significantly lower compared with the wild‑type IL‑10R1 cells. However, the phosphorylation levels in the three cell types were not significantly different. In conclusion, the deficiency in Jak1‑Stat3 signal transduction in NZW IL‑10R1 cells induces a loss in the inhibition ability of proliferation and activation as well as a migration tendency of B lymphocytes, which is hypothesized to be associated with the occurrence and development of the autoimmune disease systemic lupus erythematosus (SLE).
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Heinlen LD, McClain MT, Merrill J, et al: Clinical criteria for systemic lupus erythematosus precede diagnosis, and associated autoantibodies are present before clinical symptoms. Arthritis Rheum. 56:2344–2351. 2007. View Article : Google Scholar

2 

Beebe AM, Cua DJ and de Waal Malefyt R: The role of interleukin-10 in autoimmune disease: systemic lupus erythematosus (SLE) and multiple sclerosis (MS). Cytokine Growth Factor Rev. 13:403–412. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Houssiau FA, Lefebvre C, Vanden Berghe M, et al: Serum interleukin 10 titers in systemic lupus erythematosus reflect disease activity. Lupus. 4:393–395. 1995. View Article : Google Scholar : PubMed/NCBI

4 

Mongan AE, Ramdahin S and Warrington RJ: Interleukin-10 response abnormalities in systemic lupus erythematosus. Scand J Immunol. 46:406–412. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Mok CC and Lau CS: Pathogenesis of systemic lupus erythematosus. J Clin Pathol. 56:481–490. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Sprengers D, Stoop JN, Binda RS, et al: Induction of regulatory T-cells and interleukin-10-producing cells in non-responders to pegylated interferon-alpha therapy for chronic hepatitis B. Antivir Ther. 12:1087–1096. 2007.PubMed/NCBI

7 

Ray A, Basu S, Williams CB, et al: A novel IL-10-independent regulatory role for B cells in suppressing autoimmunity by maintenance of regulatory T cells via GITR ligand. J Immunol. 188:3188–3198. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Jankovic D, Kullberg MC, Feng CG, et al: Conventional T-bet(+)Foxp3(−) Th1 cells are the major source of host-protective regulatory IL-10 during intracellular protozoan infection. J Exp Med. 204:273–283. 2007.

9 

Liu Y, Wei SH, Ho AS, et al: Expression cloning and characterization of a human IL-10 receptor. J Immunol. 152:1821–1829. 1994.PubMed/NCBI

10 

Ho AS, Wei SH, Mui AL, et al: Functional regions of the mouse interleukin-10 receptor cytoplasmic domain. Mol Cell Biol. 15:5043–5053. 1995.PubMed/NCBI

11 

Riley JK, Takeda K, Akira S, et al: Interleukin-10 receptor signaling through the JAK-STAT pathway. Requirement for two distinct receptor-derived signals for anti-inflammatory action. J Biol Chem. 274:16513–16521. 1999. View Article : Google Scholar : PubMed/NCBI

12 

Moore KW, de Waal Malefyt R, Coffman RL, et al: Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol. 19:683–765. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Qi ZM, Wang J, Sun ZR, et al: Polymorphism of the mouse gene for the interleukin 10 receptor alpha chain (Il10ra) and its association with the autoimmune phenotype. Immunogenetics. 57:697–702. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Yin Z, Bahtiyar G, Zhang N, Liu L, Zhu P, Robert ME, McNiff J, Madaio MP and Craft J: IL-10 regulates murine lupus. J Immunol. 169:2148–2155. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Akdis CA and Blaser K: Mechanisms of interleukin-10-mediated immune suppression. Immunology. 103:131–136. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Zhou M, Ding L, Peng H, et al: Association of the interleukin-10 gene polymorphism (−1082A/G) with systemic lupus erythematosus: a meta-analysis. Lupus. 22:128–135. 2013.

17 

Donnelly RP, Dickensheets H and Finbloom DS: The interleukin-10 signal transduction pathway and regulation of gene expression in mononuclear phagocytes. J Interferon Cytokine Res. 19:563–573. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Peeva E, Venkatesh J, Michael D and Diamond B: Prolactin as a modulator of B cell function: implications for SLE. Biomed Pharmacother. 58:310–319. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Valencia-Pacheco G, Layseca-Espinosa E, Niño-Moreno P, et al: Expression and function of IL-10R in mononuclear cells from patients with systemic lupus erythematosus. Scand J Rheumatol. 35:368–378. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Teichmann LL, Kashgarian M, Weaver CT, et al: B cell-derived IL-10 does not regulate spontaneous systemic autoimmunity in MRL.Fas(lpr) mice. J Immunol. 188:678–685. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Blair PA, Chavez-Rueda KA, Evans JG, et al: Selective targeting of B cells with agonistic anti-CD40 is an efficacious strategy for the generation of induced regulatory T2-like B cells and for the suppression of lupus in MRL/lpr mice. J Immunol. 182:3492–3502. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Biswas PS, Pedicord V, Ploss A, et al: Pathogen-specific CD8 T Cell responses are directly inhibited by IL-10. J Immunol. 179:4520–4528. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Diao C, Qi Z, Du S, Cai X and Jiang Y: Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function. Mol Med Rep 10: 353-358, 2014.
APA
Diao, C., Qi, Z., Du, S., Cai, X., & Jiang, Y. (2014). Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function. Molecular Medicine Reports, 10, 353-358. https://doi.org/10.3892/mmr.2014.2147
MLA
Diao, C., Qi, Z., Du, S., Cai, X., Jiang, Y."Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function". Molecular Medicine Reports 10.1 (2014): 353-358.
Chicago
Diao, C., Qi, Z., Du, S., Cai, X., Jiang, Y."Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function". Molecular Medicine Reports 10, no. 1 (2014): 353-358. https://doi.org/10.3892/mmr.2014.2147
Copy and paste a formatted citation
x
Spandidos Publications style
Diao C, Qi Z, Du S, Cai X and Jiang Y: Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function. Mol Med Rep 10: 353-358, 2014.
APA
Diao, C., Qi, Z., Du, S., Cai, X., & Jiang, Y. (2014). Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function. Molecular Medicine Reports, 10, 353-358. https://doi.org/10.3892/mmr.2014.2147
MLA
Diao, C., Qi, Z., Du, S., Cai, X., Jiang, Y."Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function". Molecular Medicine Reports 10.1 (2014): 353-358.
Chicago
Diao, C., Qi, Z., Du, S., Cai, X., Jiang, Y."Signal transduction mechanism of the NZW type IL‑10R1 and the effect on B lymphocyte function". Molecular Medicine Reports 10, no. 1 (2014): 353-358. https://doi.org/10.3892/mmr.2014.2147
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team