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Article

Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression

  • Authors:
    • Min Lv
    • Yan Li
    • Ming-Hua Ji
    • Ming Zhuang
    • Jin-Hai Tang
  • View Affiliations / Copyright

    Affiliations: Department of Scientific Research, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, Jiangsu 210009, P.R. China, Department of Oncology, Lianyungang First People's Hospital, Lianyungang, Jiangsu 222002, P.R. China, Department of Radiotherapy, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, Jiangsu 210009, P.R. China, Department of General Surgery, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 341-346
    |
    Published online on: April 17, 2014
       https://doi.org/10.3892/mmr.2014.2161
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Abstract

Sodium hydrosulfide (NaHS) is an exogenous hydrogen sulfide (H2S)‑releasing molecule and has antitumor potential against a wide variety of human cancer types. The effect of exogenous H2S on the invasion of breast cancer and the possible underlying mechanisms remain unknown. The present study aimed to investigate the in vitro effects of H2S on transforming growth factor-β1 (TGF-β1)-induced human breast cancer cells and the associated mechanisms. MCF-7 cells were incubated with TGF-β1 to induce epithelial-mesenchymal transition (EMT) and an MTT assay was performed to detect cell viability. Flow cytometry, using propidium iodide (PI) staining, was used to determine the stages of the cell cycle. Apoptosis was detected with Annexin V-fluorescein isothiocyanate and PI double staining. Western blotting was performed to detect the protein expression of cystathionine γ-lyase (CSE, an endogenous H2S producer), phospho-p38 (a signaling protein associated with apoptosis), and SNAI1 (Snail, associated with the induction of EMT). A Boyden chamber invasion assay was performed to detect tumor invasion. The results demonstrated that when NaHS was administered to TGF-β1-treated MCF-7 cells, the cells exhibited decreased proliferation, G0/G1 phase cell cycle arrest and increased apoptosis. NaHS treatment following TGF-β1 administration also resulted in decreased cell invasion and decreased EMT, which was indicated by decreased Snail protein expression. In addition, incubation with NaHS increased endogenous CSE protein expression and decreased p38 mitogen‑activated protein kinase phosphorylation in MCF-7 cells stimulated by TGF-β1. Furthermore, the inhibition of endogenous CSE by DL-propargylglycine increased EMT in the MCF-7 cells treated with NaHS and TGF-β1. In conclusion, the present study provides insights into a novel anticancer effect of H2S on breast cancer cells through activation of the CSE/H2S pathway and decreased expression of phospho-p38.
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1 

Renga B: Hydrogen sulfide generation in mammals: the molecular biology of cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE). Inflamm Allergy Drug Targets. 10:85–91. 2011.

2 

Zhang LM, Jiang CX and Liu DW: Hydrogen sulfide attenuates neuronal injury induced by vascular dementia via inhibiting apoptosis in rats. Neurochem Res. 34:1984–1992. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Ahmad FU, Sattar MA, Rathore HA, et al: Exogenous hydrogen sulfide (H2S) reduces blood pressure and prevents the progression of diabetic nephropathy in spontaneously hypertensive rats. Ren Fail. 34:203–210. 2012.PubMed/NCBI

4 

Qipshidze N, Metreveli N, Mishra PK, Lominadze D and Tyagi SC: Hydrogen sulfide mitigates cardiac remodeling during myocardial infarction via improvement of angiogenesis. Int J Biol Sci. 8:430–441. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Olson KR, Whitfield NL, Bearden SE, et al: Hypoxic pulmonary vasodilation: a paradigm shift with a hydrogen sulfide mechanism. Am J Physiol Regul Integr Comp Physiol. 298:R51–R60. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Chattopadhyay M, Kodela R, Nath N, et al: Hydrogen sulfide-releasing NSAIDs inhibit the growth of human cancer cells: a general property and evidence of a tissue type-independent effect. Biochem Pharmacol. 83:715–722. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Chattopadhyay M, Kodela R, Nath N, Barsegian A, Boring D and Kashfi K: Hydrogen sulfide-releasing aspirin suppresses NF-κB signaling in estrogen receptor negative breast cancer cells in vitro and in vivo. Biochem Pharmacol. 83:723–732. 2012.PubMed/NCBI

8 

Frantzias J, Logan JG, Mollat P, et al: Hydrogen sulphide-releasing diclofenac derivatives inhibit breast cancer-induced osteoclastogenesis in vitro and prevent osteolysis ex vivo. Br J Pharmacol. 165:1914–1925. 2012. View Article : Google Scholar

9 

Daroqui MC, Vazquez P, Bal de Kier Joffé E, Bakin AV and Puricelli LI: TGF-β autocrine pathway and MAPK signaling promote cell invasiveness and in vivo mammary adenocarcinoma tumor progression. Oncol Rep. 28:567–575. 2012.

10 

Wendt MK, Smith JA and Schiemann WP: Transforming growth factor-β-induced epithelial-mesenchymal transition facilitates epidermal growth factor-dependent breast cancer progression. Oncogene. 29:6485–6498. 2010.

11 

Fang LP, Lin Q, Tang CS and Liu XM: Hydrogen sulfide attenuates epithelial-mesenchymal transition of human alveolar epithelial cells. Pharmacol Res. 61:298–305. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Fang L, Li H, Tang C, Geng B, Qi Y and Liu X: Hydrogen sulfide attenuates the pathogenesis of pulmonary fibrosis induced by bleomycin in rats. Can J Physiol Pharmacol. 87:531–538. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Fan HN, Wang HJ, Ren L, et al: Decreased expression of p38 MAPK mediates protective effects of hydrogen sulfide on hepatic fibrosis. Eur Rev Med Pharmacol Sci. 17:644–652. 2013.PubMed/NCBI

14 

Fang LP, Lin Q, Tang CS and Liu XM: Hydrogen sulfide suppresses migration, proliferation and myofibroblast transdifferentiation of human lung fibroblasts. Pulm Pharmacol Ther. 22:554–561. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Luo Y, Liu X, Zheng Q, et al: Hydrogen sulfide prevents hypoxia-induced apoptosis via inhibition of an H2O2-activated calcium signaling pathway in mouse hippocampal neurons. Biochem Biophys Res Commun. 425:473–477. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Zhou X and Lu X: Hydrogen sulfide inhibits high-glucose-induced apoptosis in neonatal rat cardiomyocytes. Exp Biol Med (Maywood). 238:370–374. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Bos EM, Snijder PM, Jekel H, et al: Beneficial effects of gaseous hydrogen sulfide in hepatic ischemia/reperfusion injury. Transpl Int. 25:897–908. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Pei Y, Wu B, Cao Q, Wu L and Yang G: Hydrogen sulfide mediates the anti-survival effect of sulforaphane on human prostate cancer cells. Toxicol Appl Pharmacol. 257:420–428. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Huber MA, Kraut N and Beug H: Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol. 17:548–558. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Sánchez-Tilló E, Liu Y, de Barrios O, et al: EMT-activating transcription factors in cancer: beyond EMT and tumor invasiveness. Cell Mol Life Sci. 69:3429–3456. 2012.PubMed/NCBI

21 

Ma K, Liu Y, Zhu Q, et al: H2S donor, S-propargyl-cysteine, increases CSE in SGC-7901 and cancer-induced mice: evidence for a novel anti-cancer effect of endogenous H2S? PLoS One. 6:e205252011.

22 

del Barco Barrantes I and Nebreda AR: Roles of p38 MAPKs in invasion and metastasis. Biochem Soc Trans. 40:79–84. 2012.PubMed/NCBI

23 

Chen HH, Zhou XL, Shi YL and Yang J: Roles of p38 MAPK and JNK in TGF-β1-induced human alveolar epithelial to mesenchymal transition. Arch Med Res. 44:93–98. 2013.

24 

Antoon JW, Nitzchke AM, Martin EC, et al: Inhibition of p38 mitogen-activated protein kinase alters microRNA expression and reverses epithelial-to-mesenchymal transition. Int J Oncol. 42:1139–1150. 2013.PubMed/NCBI

25 

Yang Y, Li Y, Wang K, Wang Y, Yin W and Li L: P38/NF-κB/snail pathway is involved in caffeic acid-induced inhibition of cancer stem cells-like properties and migratory capacity in malignant human keratinocyte. PLoS One. 8:e589152013.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lv M, Li Y, Ji M, Zhuang M and Tang J: Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression. Mol Med Rep 10: 341-346, 2014.
APA
Lv, M., Li, Y., Ji, M., Zhuang, M., & Tang, J. (2014). Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression. Molecular Medicine Reports, 10, 341-346. https://doi.org/10.3892/mmr.2014.2161
MLA
Lv, M., Li, Y., Ji, M., Zhuang, M., Tang, J."Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression". Molecular Medicine Reports 10.1 (2014): 341-346.
Chicago
Lv, M., Li, Y., Ji, M., Zhuang, M., Tang, J."Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression". Molecular Medicine Reports 10, no. 1 (2014): 341-346. https://doi.org/10.3892/mmr.2014.2161
Copy and paste a formatted citation
x
Spandidos Publications style
Lv M, Li Y, Ji M, Zhuang M and Tang J: Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression. Mol Med Rep 10: 341-346, 2014.
APA
Lv, M., Li, Y., Ji, M., Zhuang, M., & Tang, J. (2014). Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression. Molecular Medicine Reports, 10, 341-346. https://doi.org/10.3892/mmr.2014.2161
MLA
Lv, M., Li, Y., Ji, M., Zhuang, M., Tang, J."Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression". Molecular Medicine Reports 10.1 (2014): 341-346.
Chicago
Lv, M., Li, Y., Ji, M., Zhuang, M., Tang, J."Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression". Molecular Medicine Reports 10, no. 1 (2014): 341-346. https://doi.org/10.3892/mmr.2014.2161
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