Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2014 Volume 10 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2014 Volume 10 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β

  • Authors:
    • Lingrong Luo
    • Hong Liu
    • Zheng Dong
    • Lin Sun
    • Youming Peng
    • Fuyou Liu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA
  • Pages: 137-144
    |
    Published online on: April 17, 2014
       https://doi.org/10.3892/mmr.2014.2162
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Emerging evidence has suggested that human peritoneal mesothelial cells (HPMCs) undergo epithelial‑mesenchymal transition (EMT) in peritoneal fibrosis. The molecular mechanisms underlying peritoneal fibrosis and the key molecules involved are not yet fully elucidated. In order to enhance the understanding of peritoneal fibrosis, the present study investigated the roles of integrin‑linked kinase (ILK) and glycogen synthase kinase 3β (GSK‑3β) in high glucose‑induced phenotypic alterations of HPMCs. It was observed that HPMCs exhibited a cobblestone morphology under normal glucose conditions, whereas under high glucose conditions they had a spindle morphology. Additionally, under high glucose conditions it was found that E‑cadherin expression was decreased and vimentin expression was increased in HPMCs, suggesting HPMCs underwent EMT. ILK expression in high glucose conditions was also increased in a dose‑ and time‑dependent manner. The role of ILK in the induction of EMT in HPMCs was further investigated using small interfering RNA (siRNA). Following knockdown of ILK gene expression by siRNA, low vimentin expression as well as high E‑cadherin expression were observed, suggesting that EMT was inhibited. ILK‑knockdown also inhibited phosphorylation of GSK‑3β. These results indicate that ILK‑knockdown inhibits EMT of HPMCs through inhibition of GSK‑3β phosphorylation. These findings suggest that ILK may be used as a novel diagnostic and therapeutic target for HPMC fibrosis in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Aroeira LS, Aguilera A, Selgas R, et al: Mesenchymal conversion of mesothelial cells as a mechanism responsible for high solute transport rate in peritoneal dialysis: role of vascular endothelial growth factor. Am J Kidney Dis. 46:938–948. 2005. View Article : Google Scholar

2 

Yáñez-Mó M, Lara-Pezzi E, Selgas R, et al: Peritoneal dialysis and epithelial-to-mesenchymal transition of mesothelial cells. N Engl J Med. 348:403–413. 2003.

3 

Kim YL: Update on mechanisms of ultrafiltration failure. Perit Dial Int. 29(Suppl 2): S123–S127. 2009.PubMed/NCBI

4 

Loureiro J, Aguilera A, Selgas R, et al: Blocking TGF-β1 protects the peritoneal membrane from dialysate-induced damage. J Am Soc Nephrol. 22:1682–1695. 2011.

5 

Margetts PJ, Bonniaud P, Liu L, et al: Transient overexpression of TGF-{beta}1 induces epithelial mesenchymal transition in the rodent peritoneum. J Am Soc Nephrol. 16:425–436. 2005.

6 

Vargha R, Endemann M, Kratochwill K, et al: Ex vivo reversal of in vivo transdifferentiation in mesothelial cells grown from peritoneal dialysate effluents. Nephrol Dial Transplant. 21:2943–2947. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Wang X, Nie J, Jia Z, et al: Impaired TGF-beta signalling enhances peritoneal inflammation induced by E. coli in rats. Nephrol Dial Transplant. 25:399–412. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Lee HB and Ha H: Mechanisms of epithelial-mesenchymal transition of peritoneal mesothelial cells during peritoneal dialysis. J Korean Med Sci. 22:943–945. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Zeisberg M and Duffield JS: Resolved: EMT produces fibroblasts in the kidney. J Am Soc Nephrol. 21:1247–1253. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Liu Y: New insights into epithelial-mesenchymal transition in kidney fibrosis. J Am Soc Nephrol. 21:212–222. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Hannigan GE, Leung-Hagesteijn C, Fitz-Gibbon L, Coppolino MG, Radeva G, Filmus J, Bell JC and Dedhar S: Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Nature. 379:91–96. 1996. View Article : Google Scholar : PubMed/NCBI

12 

Legate KR, Montañez E, Kudlacek O and Fässler R: ILK, PINCH and parvin: the tIPP of integrin signaling. Nat Rev Mol Cell Biol. 7:20–31. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Dai HY, Zheng M, Lv LL, Tang RN, Ma KL, Liu D, Wu M and Liu BC: The roles of connective tissue growth factor and integrin-linked kinase in high glucose-induced phenotypic alterations of podocytes. J Cell Biochem. 113:293–301. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Li Y, Tan X, Dai C, Stolz DB, Wang D and Liu Y: Inhibition of integrin-linked kinase attenuates renal interstitial fibrosis. J Am Soc Nephrol. 20:1907–1918. 2009. View Article : Google Scholar : PubMed/NCBI

15 

de Teixeira VP, Blattner SM, Li M, et al: Functional consequences of integrin-linked kinase activation in podocyte damage. Kidney Int. 67:514–523. 2005.PubMed/NCBI

16 

Kretzler M, Teixeira VP, Unschuld PG, et al: Integrin-linked kinase as a candidate downstream effector in proteinuria. FASEB J. 15:1843–1845. 2001.PubMed/NCBI

17 

Guo L, Sanders PW, Woods A and Wu C: The distribution and regulation of integrin-linked kinase in normal and diabetic kidneys. Am J Pathol. 159:1735–1742. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Li Y, Yang J, Dai C, et al: Role for integrin-linked kinase in mediating tubular epithelial to mesenchymal transition and renal interstitial fibrogenesis. J Clin Invest. 112:503–516. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Persad S, Attwell S, Gray V, Mawji N, Deng JT, Leung D, et al: Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinaseactivity and amino acids arginine 211 and serine 343. J Biol Chem. 276:27462–27469. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Delcommenne M, Tan C, Gray V, Rue L, Woodgett J and Dedhar S: Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. Proc Natl Acad Sci USA. 95:11211–11216. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Vi L, de Lasa C, DiGuglielmo GM, et al: Integrin-linked kinase is required for TGF-β1 induction of dermal myofibroblast differentiation. J Invest Dermatol. 131:586–593. 2011.

22 

Li Y, Yang J, Luo JH, et al: Tubular epithelial cell dedifferentiation is driven by the helix-loop-helix transcriptional inhibitor Id1. J Am Soc Nephrol. 18:449–460. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Smeeton J, Zhang X, Bulus N, et al: Integrin-linked kinase regulates p38 MAPK-dependent cell cycle arrest in ureteric bud development. Development. 137:3233–3243. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Liu XC, Liu BC, Zhang XL, et al: Role of ERK1/2 and PI3-K in the regulation of CTGF-induced ILK expression in HK-2 cells. Clin Chim Acta. 382:89–94. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Stylianou E, Jenner LA, Davies M, Coles GA and Williams JD: Isolation, culture and characterization of human peritoneal mesothelial cells. Kidney Int. 37:1563–1570. 1990. View Article : Google Scholar : PubMed/NCBI

26 

Rougier JP, Moullier P, Piedagnel R and Ronco PM: Hyperosmolality suppresses but TGF beta 1 increases MMP9 in human peritoneal mesothelial cells. Kidney Int. 51:337–347. 1997. View Article : Google Scholar : PubMed/NCBI

27 

Blattner SM and Kretzler M: Integrin-linked kinase in renal disease: connecting cell-matrix interaction to the cytoskeleton. Curr Opin Nephrol Hypertens. 14:404–410. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Dai C, Stolz DB, Bastacky SI, St-Arnaud R, Wu C, Dedhar S and Liu Y: Essential role of integrin-linked kinase in podocyte biology: Bridging the integrin and slit diaphragm signaling. J Am Soc Nephrol. 17:2164–2175. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Kang YS, Li Y, Dai C, Kiss LP, Wu C and Liu Y: Inhibition of integrin-linked kinase blocks podocyte epithelial-mesenchymal transition and ameliorates proteinuria. Kidney Int. 78:363–373. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Li Y, Dai C, Wu C and Liu Y: PINCH-1 promotes tubular epithelial-to-mesenchymal transition by interacting with integrin-linked kinase. J Am Soc Nephrol. 18:2534–2543. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Hu K, Wu C, Mars WM and Liu Y: Tissue-type plasminogen activator promotes murine myofibroblast activation through LDL receptor-related protein 1-mediated integrin signaling. J Clin Invest. 117:3821–3832. 2007.

32 

Guo L and Wu C: Regulation of fibronectin matrix deposition and cell proliferation by the PINCH-ILK-CH-ILKBP complex. FASEB J. 16:1298–1300. 2002.PubMed/NCBI

33 

Tu Y, Huang Y, Zhang Y, Hua Y and Wu C: A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. J Cell Biol. 153:585–598. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Nikolopoulos SN and Turner CE: Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem. 277:1568–1575. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Troussard AA, Costello P, Yoganathan TN, Kumagai S, Roskelley CD and Dedhar S: The integrin linked kinase (ILK) induces an invasive phenotype via AP-1 transcription factor-dependent upregulation of matrix metalloproteinase 9 (MMP-9). Oncogene. 19:5444–5452. 2000. View Article : Google Scholar : PubMed/NCBI

36 

D’Amico M, Hulit J, Amanatullah DF, et al: The integrin-linked kinase regulates the cyclin D1 gene through glycogen synthase kinase 3beta and cAMP-responsive element-binding protein-dependent pathways. J Biol Chem. 275:32649–32657. 2000.PubMed/NCBI

37 

Joshi MB, Ivanov D, Philippova M, Erne P and Resink TJ: Integrin-linked kinase is an essential mediator for T-cadherin-dependent signaling via Akt and GSK3beta in endothelial cells. FASEB J. 21:3083–3095. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Sellin JH, Umar S, Xiao J and Morris AP: Increased beta-catenin expression and nuclear translocation accompany cellular hyperproliferation in vivo. Cancer Res. 61:2899–2906. 2001.PubMed/NCBI

39 

Yang Y, Guo L, Blattner SM, Mundel P, Kretzler M and Wu C: Formation and phosphorylation of the PINCH-1-integrin linked kinase-alpha-parvin complex are important for regulation of renal glomerular podocyte adhesion, architecture, and survival. J Am Soc Nephrol. 16:1966–1976. 2005. View Article : Google Scholar : PubMed/NCBI

40 

Cortés JC, Montalvo EA, Muñiz J, Mornet D, Garrido E, Centeno F and Cisneros B: Dp71f modulates GSK3-beta recruitment to the beta1-integrin adhesion complex. Neurochem Res. 34:438–444. 2009.PubMed/NCBI

41 

Kalra J, Sutherland BW, Stratford AL, et al: Suppression of Her2/neu expression through ILK inhibition is regulated by a pathway involving TWIST and YB-1. Oncogene. 29:6343–6356. 2010. View Article : Google Scholar : PubMed/NCBI

42 

del Nogal M, Luengo A, Olmos G, Lasa M, Rodriguez-Puyol D, Rodriguez-Puyol M and Calleros L: Balance between apoptosis or survival induced by changes in extracellular-matrix composition in human mesangial cells: a key role for ILK-NfκB pathway. Apoptosis. 17:1261–1274. 2012.

43 

Papachristou DJ, Gkretsi V, Rao UN, et al: Expression of integrin-linked kinase and its binding partners in chondrosarcoma: association with prognostic significance. Eur J Cancer. 44:2518–2525. 2008. View Article : Google Scholar

44 

McDonald PC, Fielding AB and Dedhar S: Integrin-linked kinase - essential roles in physiology and cancer biology. J Cell Sci. 121:3121–3132. 2008. View Article : Google Scholar : PubMed/NCBI

45 

Fielding AB, Dobreva I, McDonald PC, Foster LJ and Dedhar S: Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. J Cell Biol. 180:681–689. 2008. View Article : Google Scholar : PubMed/NCBI

46 

Shin SY, Rath O, Zebisch A, Choo SM, Kolch W and Cho KH: Functional roles of multiple feedback loops in extracellular signal-regulated kinase and Wnt signaling pathways that reg-ulate epithelial-mesenchymal transition. Cancer Res. 70:6715–6724. 2010. View Article : Google Scholar

47 

Acloque H, Thiery JP and Nieto MA: The physiology and pathology of the EMT. Meeting on the epithelial-mesenchymal transition. EMBO Rep. 9:322–326. 2008. View Article : Google Scholar : PubMed/NCBI

48 

Xing Y, Qi J, Deng S, Wang C, Zhang L and Chen J: Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition. Exp Cell Res. 319:2058–2072. 2013. View Article : Google Scholar : PubMed/NCBI

49 

McDonald PC, Oloumi A, Mills J, et al: Rictor and integrin-linked kinase interact and regulate Akt phosphorylation and cancer cell survival. Cancer Res. 68:1618–1624. 2008. View Article : Google Scholar : PubMed/NCBI

50 

Doble BW and Woodgett JR: Role of glycogen synthase kinase-3 in cell fate and epithelial-mesenchymal transitions. Cells Tissues Organs. 185:73–84. 2007. View Article : Google Scholar : PubMed/NCBI

51 

McPhee TR, McDonald PC, Oloumi A and Dedhar S: Integrin-linked kinase regulates E-cadherin expression through PARP-1. Dev Dyn. 237:2737–2747. 2008. View Article : Google Scholar : PubMed/NCBI

52 

Maseki S, Ijichi K, Tanaka H, et al: Acquisition of EMT phenotype in the gefitinib-resistant cells of a head and neck squamous cell carcinoma cell line through Akt/GSK-3β/snail signalling pathway. Br J Cancer. 106:1196–1204. 2012.PubMed/NCBI

53 

Wu C and Dedhar S: Integrin-linked kinase (ILK) and its interactors: a new paradigm for the coupling of extracellular matrix to actin cytoskeleton and signaling complexes. J Cell Biol. 155:505–510. 2001. View Article : Google Scholar : PubMed/NCBI

54 

Radeva G, Petrocelli T, Behrend E, Leung-Hagesteijn C, Filmus J, Slingerland J and Dedhar S: Overexpression of the integrin-linked kinase promotes anchorage-independent cell cycle progression. J Biol Chem. 272:13937–13944. 1997. View Article : Google Scholar : PubMed/NCBI

55 

Tan C, Costello P, Sanghera J, Dominguez D, Baulida J, de Herreros AG and Dedhar S: Inhibition of integrin linked kinase (ILK) suppresses beta-catenin-Lef/Tcf-dependent transcription and expression of the E-cadherin repressor, snail, in APC−/− human colon carcinoma cells. Oncogene. 20:133–140. 2001.PubMed/NCBI

56 

Barisoni L, Mokrzycki M, Sablay L, Nagata M, Yamase H and Mundel P: Podocyte cell cycle regulation and proliferation in collapsing glomerulopathies. Kidney Int. 58:137–143. 2000. View Article : Google Scholar : PubMed/NCBI

57 

Petermann AT, Pippin J, Hiromura K, et al: Mitotic cell cycle proteins increase in podocytes despite lack of proliferation. Kidney Int. 63:113–122. 2003. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Luo L, Liu H, Dong Z, Sun L, Peng Y and Liu F: Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β. Mol Med Rep 10: 137-144, 2014.
APA
Luo, L., Liu, H., Dong, Z., Sun, L., Peng, Y., & Liu, F. (2014). Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β. Molecular Medicine Reports, 10, 137-144. https://doi.org/10.3892/mmr.2014.2162
MLA
Luo, L., Liu, H., Dong, Z., Sun, L., Peng, Y., Liu, F."Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β". Molecular Medicine Reports 10.1 (2014): 137-144.
Chicago
Luo, L., Liu, H., Dong, Z., Sun, L., Peng, Y., Liu, F."Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β". Molecular Medicine Reports 10, no. 1 (2014): 137-144. https://doi.org/10.3892/mmr.2014.2162
Copy and paste a formatted citation
x
Spandidos Publications style
Luo L, Liu H, Dong Z, Sun L, Peng Y and Liu F: Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β. Mol Med Rep 10: 137-144, 2014.
APA
Luo, L., Liu, H., Dong, Z., Sun, L., Peng, Y., & Liu, F. (2014). Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β. Molecular Medicine Reports, 10, 137-144. https://doi.org/10.3892/mmr.2014.2162
MLA
Luo, L., Liu, H., Dong, Z., Sun, L., Peng, Y., Liu, F."Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β". Molecular Medicine Reports 10.1 (2014): 137-144.
Chicago
Luo, L., Liu, H., Dong, Z., Sun, L., Peng, Y., Liu, F."Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK‑3β". Molecular Medicine Reports 10, no. 1 (2014): 137-144. https://doi.org/10.3892/mmr.2014.2162
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team