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Article

Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice

  • Authors:
    • Xue‑Fei Yang
    • Yi He
    • Hai‑Yuan Li
    • Xin Liu
    • Huan Chen
    • Jian‑Bang Liu
    • Wen‑Jun Ji
    • Bing Wang
    • Li‑Na Chen
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 555-559
    |
    Published online on: April 24, 2014
       https://doi.org/10.3892/mmr.2014.2164
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Abstract

Fulminant hepatic failure is a severe clinical syndrome associated with a high rate of patient mortality. Recent studies have shown that in addition to its hematopoietic effect, erythropoietin (EPO) has multiple protective effects and exhibits antiapoptotic, antioxidant and anti‑inflammatory activities. The present study aimed to determine the hepatoprotective effect of EPO and to elucidate the underlying mechanisms using a D‑galactosamine (D‑GalN)/lipopolysaccharide (LPS)‑induced model of acute liver injury. Experimental groups of mice were administered with various doses of EPO (1,000, 3,000 or 10,000 U/kg, intraperitoneal) once per day for 3 days, prior to injection with D‑GalN (700 mg/kg)/LPS (10 µg/kg). Mice were sacrificed 8 h after treatment with D‑GalN/LPS. Liver function and histopathology, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH‑Px) activities and EPO receptor (EPOR) and phosphatidylinositol 3‑kinase (PI3K) mRNA expression were evaluated. D‑GalN/LPS administration markedly induced liver injury, as evidenced by elevated levels of serum aminotransferases, as well as histopathological changes. Compared with the D‑GalN/LPS group, pretreatment with EPO significantly decreased the levels of aspartate aminotransferase, alanine aminotransferase and MDA, and increased the activities of SOD and GSH‑Px. Furthermore, the protective effects of EPO were paralleled by an upregulation in the mRNA expression of EPOR and PI3K. These data suggest that EPO can ameliorate D‑GalN/LPS‑induced acute liver injury by reducing oxidative stress and upregulating the mRNA expression of EPOR and PI3K.
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Copy and paste a formatted citation
Spandidos Publications style
Yang XF, He Y, Li HY, Liu X, Chen H, Liu JB, Ji WJ, Wang B and Chen LN: Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice. Mol Med Rep 10: 555-559, 2014.
APA
Yang, X., He, Y., Li, H., Liu, X., Chen, H., Liu, J. ... Chen, L. (2014). Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice. Molecular Medicine Reports, 10, 555-559. https://doi.org/10.3892/mmr.2014.2164
MLA
Yang, X., He, Y., Li, H., Liu, X., Chen, H., Liu, J., Ji, W., Wang, B., Chen, L."Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice". Molecular Medicine Reports 10.1 (2014): 555-559.
Chicago
Yang, X., He, Y., Li, H., Liu, X., Chen, H., Liu, J., Ji, W., Wang, B., Chen, L."Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice". Molecular Medicine Reports 10, no. 1 (2014): 555-559. https://doi.org/10.3892/mmr.2014.2164
Copy and paste a formatted citation
x
Spandidos Publications style
Yang XF, He Y, Li HY, Liu X, Chen H, Liu JB, Ji WJ, Wang B and Chen LN: Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice. Mol Med Rep 10: 555-559, 2014.
APA
Yang, X., He, Y., Li, H., Liu, X., Chen, H., Liu, J. ... Chen, L. (2014). Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice. Molecular Medicine Reports, 10, 555-559. https://doi.org/10.3892/mmr.2014.2164
MLA
Yang, X., He, Y., Li, H., Liu, X., Chen, H., Liu, J., Ji, W., Wang, B., Chen, L."Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice". Molecular Medicine Reports 10.1 (2014): 555-559.
Chicago
Yang, X., He, Y., Li, H., Liu, X., Chen, H., Liu, J., Ji, W., Wang, B., Chen, L."Hepatoprotective effects of erythropoietin on D‑galactosamine/lipopolysaccharide‑induced fulminant hepatic failure in mice". Molecular Medicine Reports 10, no. 1 (2014): 555-559. https://doi.org/10.3892/mmr.2014.2164
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