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Article

Role of CIP2A in the antitumor effect of bortezomib in colon cancer

  • Authors:
    • Yayun Ding
    • Yueguo Wang
    • Shaoqing Ju
    • Xinghua  Wu
    • Wencai Zhu
    • Feng Shi
    • Liping Mao
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Nantong University, Nantong, Jiangsu 226001, P.R. China, Laboratory Department, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Laboratory Department, Nantong Third People's Hospital, Nantong, Jiangsu 226001, P.R. China
  • Pages: 387-392
    |
    Published online on: April 24, 2014
       https://doi.org/10.3892/mmr.2014.2173
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Abstract

Cancerous inhibitor of protein phosphatase 2A (CIP2A) has been identified as an oncoprotein that is able to promote the proliferation of cancer cells. The role of CIP2A in the anticancer activity of bortezomib in colon cancer remains to be elucidated. In the present study, the antitumor effect of bortezomib was investigated and the role of CIP2A in determining the effect on colon cancer cells was identified. In the present study, bortezomib demonstrated an antitumor effect, as observed by WST‑1 assay and flow cytometry. In addition, the mRNA and protein level of CIP2A was inhibited in a dose‑dependent manner by bortezomib with quantitative PCR (qPCR) and western blotting. Furthermore, the inhibition of CIP2A with small interfering RNA by treatment with bortezomib inhibited proliferation, increased apoptosis and attenuated the invasion of the cells. Finally, the in vivo data demonstrated that bortezomib was able to decrease the growth of tumors, and that CIP2A was downregulated in the LoVo tumors treated with bortezomib. Therefore, CIP2A was shown to be important in the bortezomib‑induced inhibitory effect on colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Ding Y, Wang Y, Ju S, Wu X, Zhu W, Shi F and Mao L: Role of CIP2A in the antitumor effect of bortezomib in colon cancer. Mol Med Rep 10: 387-392, 2014.
APA
Ding, Y., Wang, Y., Ju, S., Wu, X., Zhu, W., Shi, F., & Mao, L. (2014). Role of CIP2A in the antitumor effect of bortezomib in colon cancer. Molecular Medicine Reports, 10, 387-392. https://doi.org/10.3892/mmr.2014.2173
MLA
Ding, Y., Wang, Y., Ju, S., Wu, X., Zhu, W., Shi, F., Mao, L."Role of CIP2A in the antitumor effect of bortezomib in colon cancer". Molecular Medicine Reports 10.1 (2014): 387-392.
Chicago
Ding, Y., Wang, Y., Ju, S., Wu, X., Zhu, W., Shi, F., Mao, L."Role of CIP2A in the antitumor effect of bortezomib in colon cancer". Molecular Medicine Reports 10, no. 1 (2014): 387-392. https://doi.org/10.3892/mmr.2014.2173
Copy and paste a formatted citation
x
Spandidos Publications style
Ding Y, Wang Y, Ju S, Wu X, Zhu W, Shi F and Mao L: Role of CIP2A in the antitumor effect of bortezomib in colon cancer. Mol Med Rep 10: 387-392, 2014.
APA
Ding, Y., Wang, Y., Ju, S., Wu, X., Zhu, W., Shi, F., & Mao, L. (2014). Role of CIP2A in the antitumor effect of bortezomib in colon cancer. Molecular Medicine Reports, 10, 387-392. https://doi.org/10.3892/mmr.2014.2173
MLA
Ding, Y., Wang, Y., Ju, S., Wu, X., Zhu, W., Shi, F., Mao, L."Role of CIP2A in the antitumor effect of bortezomib in colon cancer". Molecular Medicine Reports 10.1 (2014): 387-392.
Chicago
Ding, Y., Wang, Y., Ju, S., Wu, X., Zhu, W., Shi, F., Mao, L."Role of CIP2A in the antitumor effect of bortezomib in colon cancer". Molecular Medicine Reports 10, no. 1 (2014): 387-392. https://doi.org/10.3892/mmr.2014.2173
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