Epithelial‑mesenchymal transition and apoptosis of renal tubular epithelial cells are associated with disease progression in patients with IgA nephropathy

Yao,Junxia; Ke,Zunji; Wang,Xiaoyan; Peng,Feng; Li,Bin; Wu,Renliang

doi:10.3892/mmr.2014.2179

July-2014 Volume 10 Issue 1

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July-2014 Volume 10 Issue 1

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Article Open Access

Epithelial‑mesenchymal transition and apoptosis of renal tubular epithelial cells are associated with disease progression in patients with IgA nephropathy

Authors:
- Junxia Yao
- Zunji Ke
- Xiaoyan Wang
- Feng Peng
- Bin Li
- Renliang Wu
View Affiliations / Copyright

Affiliations: Institute of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Pathology, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China

Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 39-44
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Published online on: April 24, 2014

https://doi.org/10.3892/mmr.2014.2179
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Abstract

The aim of the present study was to examine the effects of epithelial‑mesenchymal transition (EMT) and apoptosis of renal tubular epithelial cells on the prognosis of immunoglobulin A (IgA) nephropathy. Renal biopsy tissues from 74 cases of IgA nephropathy were divided into a mild mesangial proliferation group (27 cases), a focal hyperplasia group (28 cases) and a proliferative sclerosis group (19 cases). The blood pressure, serum creatinine and 24 h urinary protein excretion of all patients were detected. To define EMT, α‑smooth muscle actin (α‑SMA), vimentin and collagen fibers were assessed. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). The blood pressure, serum creatinine and 24 h urinary protein excretion of patients with IgA nephropathy altered with increasing pathological grade. All clinical indices of patients in the proliferative sclerosis group were higher than those of the other two groups, and the 24 h urinary protein excretion of the focal hyperplasia group was statistically higher than that of the mild mesangial proliferation group. The expression of tubular interstitial α‑SMA, vimentin and collagen fibers increased with the pathological grade and was closely correlated with clinical indices, including collagen fibers and 24 h urinary protein excretion. TUNEL‑positive cells increased with the exacerbation of pathological changes. The EMT and apoptosis of renal tubular epithelial cells reflected the clinical severity of IgA nephropathy. α‑SMA, vimentin and the apoptotic index may be used as important markers for evaluating the prognosis of IgA nephropathy.

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1	Berger J and Hinglais N: Intercapillary deposits of IgA-IgG. J Urol Nephrol (Paris). 74:694–695. 1968.(In French).
2	Mubarak M: IgA nephropathy: an update on pathogenesis and classification. J Coll Physicians Surg Pak. 21:230–233. 2011.PubMed/NCBI
3	Bogenschütz O, Bohle A, Batz C, et al: IgA nephritis: on the importance of morphological and clinical parameters in the long-term prognosis of 239 patients. Am J Nephrol. 10:137–147. 1990.PubMed/NCBI
4	Liu Y: Renal fibrosis: new insights into the pathogenesis and therapeutics. Kidney Int. 69:213–217. 2006. View Article : Google Scholar : PubMed/NCBI
5	Zeisberg M and Kalluri R: The role of epithelial-to-mesenchymal transition in renal fibrosis. J Mol Med (Berl). 82:175–181. 2004. View Article : Google Scholar : PubMed/NCBI
6	Chevalier RL: Obstructive nephropathy: towards biomarker discovery and gene therapy. Nat Clin Pract Nephrol. 2:157–168. 2006. View Article : Google Scholar : PubMed/NCBI
7	Hills CE and Squires PE: TGF-beta1-induced epithelial-to-mesenchymal transition and therapeutic intervention in diabetic nephropathy. Am J Nephrol. 31:68–74. 2010. View Article : Google Scholar : PubMed/NCBI
8	Pozdzik AA, Salmon IJ, Debelle FD, et al: Aristolochic acid induces proximal tubule apoptosis and epithelial to mesenchymal transformation. Kidney Int. 73:595–607. 2008. View Article : Google Scholar : PubMed/NCBI
9	Lee HS, Lee MS, Lee SM, et al: Histological grading of IgA nephropathy predicting renal outcome: revisiting H. S. Lee’s glomerular grading system. Nephrol Dial Transplant. 20:342–348. 2005.PubMed/NCBI
10	Glassock RJ: The pathogenesis of IgA nephropathy. Curr Opin Nephrol Hypertens. 20:153–160. 2011. View Article : Google Scholar : PubMed/NCBI
11	Li PK, Ho KK, Szeto CC, Yu L and Lai FM: Prognostic indicators of IgA nephropathy in the Chinese - clinical and pathological perspectives. Nephrol Dial Transplant. 17:64–69. 2002. View Article : Google Scholar : PubMed/NCBI
12	Manno C, Strippoli GF, D’Altri C, Torres D, Rossini M and Schena FP: A novel simpler histological classification for renal survival in IgA nephropathy: a retrospective study. Am J Kidney Dis. 49:763–775. 2007. View Article : Google Scholar : PubMed/NCBI
13	Bellur SS, Troyanov S, Cook HT and Roberts IS; Working Group of International IgA Nephropathy Network and Renal Pathology Society. Immunostaining findings in IgA nephropathy: correlation with histology and clinical outcome in the Oxford classification patient cohort. Nephrol Dial Transplant. 26:2533–2536. 2011. View Article : Google Scholar
14	D’Amico G: Natural history of idiopathic IgA nephropathy and factors predictive of disease outcome. Semin Nephrol. 24:179–196. 2004.PubMed/NCBI
15	Strutz F, Okada H, Lo CW, et al: Identification and characterization of a fibroblast marker: FSP1. J Cell Biol. 130:393–405. 1995. View Article : Google Scholar : PubMed/NCBI
16	Okada H, Danoff TM, Kalluri R and Neilson EG: Early role of Fsp1 in epithelial-mesenchymal transformation. Am J Physiol. 273:F563–F274. 1997.PubMed/NCBI
17	Fan JM, Ng YY, Hill PA, et al: Transforming growth factor-beta regulates tubular epithelial-myofibroblast transdifferentiation in vitro. Kidney Int. 56:1455–1467. 1999. View Article : Google Scholar : PubMed/NCBI
18	Rastaldi MP, Ferrario F, Giardino L, et al: Epithelial-mesenchymal transition of tubular epithelial cells in human renal biopsies. Kidney Int. 62:137–146. 2002. View Article : Google Scholar : PubMed/NCBI