Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2014 Volume 10 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2014 Volume 10 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis

  • Authors:
    • Zhenying Zhang
    • Zhen Yu
    • Pan Tian
    • Suchun Hou
    • Shixin Han
    • Xuejing Tan
    • Yongjun Piao
    • Xiaoming Liu
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China, Dalian Dermatosis Hospital, Dalian, Liaoning, P.R. China, Department of Dermatology, Hong Kong University-Shenzhen Hospital, Shenzhen, Guangdong 518053, P.R. China
  • Pages: 479-485
    |
    Published online on: May 2, 2014
       https://doi.org/10.3892/mmr.2014.2203
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Psoriasis is a T cell‑mediated, chronic, relapsing and inflammatory cutaneous disorder. The dysfunctional activity of T cells in patients with psoriasis is attributed to bone marrow hematopoietic stem cells (BMHSCs). To understand the pathogenic roles of BMHSCs in psoriasis, a differential gene expression analysis was performed using suppression subtractive hybridization of the BMHSCs from a patient with psoriasis and a healthy control. Using a cDNA array dot blot screening to screen 600 genes from forward‑ and reverse‑subtracted cDNA libraries, 17 differentially‑expressed sequence tags (ESTs) were identified. The genes within the ESTs were observed to be the homologs of genes that are involved in various cellular processes, including hormone signaling, RNA catabolism, protein ADP DNA base melting, transcriptional regulation, cell cycle regulation and metabolism. CD45, which was overexpressed in the psoriatic BMHSCs, was further analyzed using relative quantitative polymerase chain reaction. In addition, the levels of CD45 in the peripheral blood cells (PBCs) of the patients with psoriasis were markedly increased and closely associated with disease severity. An abnormality of hematopoietic progenitor cells, e.g., CD45 overexpression, may be transferred to PBCs via hematopoiesis, and may account for the psoriasis‑inducing properties of activated T cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Nickoloff BJ: The immunologic and genetic basis of psoriasis. Arch Dermatol. 135:1104–1110. 2000.PubMed/NCBI

2 

Sabat R, Philipp S, Höflich C, et al: Immunopathogenesis of psoriasis. Exp Dermatol. 16:779–798. 2007. View Article : Google Scholar

3 

van Lingen RG, van de Kerkhof PC, de Jong EM, et al: Reduced CD26 bright expression of peripheral blood CD8+ T-cell subsets in psoriatic patients. Exp Dermatol. 17:343–348. 2008.PubMed/NCBI

4 

Berth-Jones J: The use of ciclosporin in psoriasis. J Dermatolog Treat. 16:258–277. 2005. View Article : Google Scholar

5 

Krueger JG, Wolfe JT, Nabeya RT, et al: Successful ultraviolet B treatment of psoriasis is accompanied by a reversal of keratinocyte pathology and by selective depletion of intraepidermal T cells. J Exp Med. 182:2057–2068. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Gottlieb SL, Gilleaudeau P, Johnson R, et al: Response of psoriasis to a lymphocyte-selective toxin (DAB389IL-2) suggests a primary immune, but not keratinocyte, pathogenic basis. Nat Med. 1:442–447. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Abrams JR, Kelley SL, Hayes E, et al: Blockade of T lymphocyte costimulation with cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (CTLA4Ig) reverses the cellular pathology of psoriatic plaques, including the activation of keratinocytes, dendritic cells, and endothelial cells. J Exp Med. 192:681–694. 2000. View Article : Google Scholar

8 

Gordon KB, Papp KA, Hamilton TK, et al: Efalizumab for patients with moderate to severe plaque psoriasis: a randomized controlled trial. JAMA. 290:3073–3080. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Tomfohrde J, Silverman A, Barnes R, et al: Gene for familial psoriasis susceptibility mapped to the distal end of human chromosome 17q. Science. 264:1141–1145. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Hüffmeier U, Steffens M, Burkhardt H, et al: Evidence for susceptibility determinant(s) to psoriasis vulgaris in or near PTPN22 in German patients. J Med Genet. 43:517–522. 2006.PubMed/NCBI

11 

Bowcock AM: The genetics of psoriasis and autoimmunity. Annu Rev Genomics Hum Genet. 6:93–122. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Galić Z, Kitchen SG, Subramanian A, et al: Generation of T lineage cells from human embryonic stem cells in a feeder free System. Stem Cells. 27:100–107. 2009.PubMed/NCBI

13 

Kondo M, Weissman IL and Akashi K: Identification of clonogenic common lymphoid progenitors in mouse bone marrow. Cell. 91:661–672. 1997. View Article : Google Scholar : PubMed/NCBI

14 

Petrie HT and Zúñiga-Pflücker JC: Zoned out: functional mapping of stromal signaling microenvironments in the thymus. Annu Rev Immunol. 25:649–679. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Petrie HT: Cell migration and the control of post-natal T-cell lymphopoiesis in the thymus. Nat Rev Immunol. 3:859–866. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Zhang K, Li X, Yin G, et al: Functional characterization of T cells differentiated in vitro from bone marrow-derived CD34 cells of psoriatic patients with family history. Exp Dermatol. 19:e128–e135. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Zhang K, Zhang R, Li X, et al: The mRNA expression and promoter methylation status of the p16 gene in colony-forming cells with high proliferative potential in patients with psoriasis. Clin Exp Dermatol. 32:702–708. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Lacaud G, Gore L, Kennedy M, et al: Runx1 is essential for hematopoietic commitment at the hemangioblast stage of development in vitro. Blood. 100:458–466. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Helms C, Cao L, Krueger JG, et al: A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated with susceptibility to psoriasis. Nat Genet. 35:349–356. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Kanamori H, Tanaka M, Kawaguchi H, et al: Resolution of psoriasis following allogeneic bone marrow transplantation for chronic myelogenous leukemia: case report and review of the literature. Am J Hematol. 71:41–44. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Woods AC and Mant MJ: Amelioration of severe psoriasis with psoriatic arthritis for 20 years after allogeneic haematopoietic stem cell transplantation. Ann Rheum Dis. 65:6972006.PubMed/NCBI

22 

Snowden JA and Heaton DC: Development of psoriasis after syngeneic bone marrow transplant from psoriatic donor: further evidence for adoptive autoimmunity. Br J Dermatol. 137:130–132. 1997. View Article : Google Scholar : PubMed/NCBI

23 

Byth KF, Conroy LA, Howlett S, et al: CD45-null transgenic mice reveal a positive regulatory role for CD45 in early thymocyte development, in the selection of CD4+CD8+thymocytes, and B cell maturation. J Exp Med. 183:1707–1718. 1996.PubMed/NCBI

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)). Method. 25:402–408. 2001.

25 

Ward V, Hennig BJ, Hirai K, et al: Geographical distribution and disease associations of the CD45 exon 6 138G variant. Immunogenetics. 58:235–239. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Kishihara K, Penninger J, Wallace VA, et al: Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell. 74:143–156. 1993. View Article : Google Scholar : PubMed/NCBI

27 

Hermiston ML, Xu Z and Weiss A: CD45: a critical regulator of signaling thresholds in immune cells. Annu Rev Immunol. 21:107–137. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Pingel JT and Thomas ML: Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation. Cell. 58:1055–1065. 1989. View Article : Google Scholar : PubMed/NCBI

29 

Weaver CT, Pingel JT, Nelson JO, et al: CD8+ T-cell clones dificient in the expression of the CD45 protein tyrosine phosphatase have impaired responses to T-cell receptor stimuli. Mol Cell Biol. 11:4415–4422. 1991.

30 

Koretzky GA, Kohmetscher MA, Kadleck T, et al: Restoration of T cell receptor-mediated signal transduction by transfection of CD45 cDNA into a CD45-deficient variant of the Jurkat T cell line. J Immunol. 149:1138–1142. 1992.PubMed/NCBI

31 

Montoya M, Dawes R, Reid D, Lee LN, Piercy J, Borrow P, Tchilian EZ and Beverley PC: CD45 is required for type I IFN production by dendritic cells. Eur J Immunol. 36:2150–2158. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Shen F, Xu XL, Graf LH, et al: CD45-cross-linking stimulates IFN-gamma production in NK cells. J Immunol. 154:644–652. 1995.PubMed/NCBI

33 

Dawes R, Petrova S, Liu Z, et al: Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 176:3417–3425. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Takahashi H, Tsuji H, Hashimoto Y, Ishida-Yamamoto A and Iizuka H: Serum cytokines and growth factor levels in Japanese patients with psoriasis. Clin Exp Dermatol. 35:645–649. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Szegedi A, Aleksza M, Gonda A, et al: Elevated rate of Thelper1 (T(H)1) lymphocytes and serum IFN-gamma levels in psoriatic patients. Immunol Lett. 86:277–280. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Arican Ozer, Aral Murat, Sasmaz Sezai, et al: Serum levels of TNF-alpha, IFN-gamma, IL-6, IL-8, IL-12, IL-17, and IL-18 in patients with active psoriasis and correlation with disease severity. Mediators Inflamm. 24:273–279. 2005. View Article : Google Scholar : PubMed/NCBI

37 

Zhang K, Zhang R, Li X, et al: Promoter methylation status of p15 and p21 genes in HPP-CFCs of bone marrow of patients with psoriasis. Eur J Dermatol. 9:141–146. 2009.PubMed/NCBI

38 

Yin G, Li J, Wan Y, et al: Abnormality of RUNX1 signal transduction in psoriatic CD34+ bone marrow cells. Br J Dermatol. 164:1043–1051. 2011.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Yu Z, Tian P, Hou S, Han S, Tan X, Piao Y and Liu X: Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis. Mol Med Rep 10: 479-485, 2014.
APA
Zhang, Z., Yu, Z., Tian, P., Hou, S., Han, S., Tan, X. ... Liu, X. (2014). Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis. Molecular Medicine Reports, 10, 479-485. https://doi.org/10.3892/mmr.2014.2203
MLA
Zhang, Z., Yu, Z., Tian, P., Hou, S., Han, S., Tan, X., Piao, Y., Liu, X."Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis". Molecular Medicine Reports 10.1 (2014): 479-485.
Chicago
Zhang, Z., Yu, Z., Tian, P., Hou, S., Han, S., Tan, X., Piao, Y., Liu, X."Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis". Molecular Medicine Reports 10, no. 1 (2014): 479-485. https://doi.org/10.3892/mmr.2014.2203
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Yu Z, Tian P, Hou S, Han S, Tan X, Piao Y and Liu X: Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis. Mol Med Rep 10: 479-485, 2014.
APA
Zhang, Z., Yu, Z., Tian, P., Hou, S., Han, S., Tan, X. ... Liu, X. (2014). Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis. Molecular Medicine Reports, 10, 479-485. https://doi.org/10.3892/mmr.2014.2203
MLA
Zhang, Z., Yu, Z., Tian, P., Hou, S., Han, S., Tan, X., Piao, Y., Liu, X."Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis". Molecular Medicine Reports 10.1 (2014): 479-485.
Chicago
Zhang, Z., Yu, Z., Tian, P., Hou, S., Han, S., Tan, X., Piao, Y., Liu, X."Differentially‑expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis". Molecular Medicine Reports 10, no. 1 (2014): 479-485. https://doi.org/10.3892/mmr.2014.2203
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team