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Article Open Access

Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells

  • Authors:
    • Sumei Lu
    • Liang Yu
    • Yakui Mu
    • Juke Ma
    • Jiajun Tian
    • Wei Xu
    • Haibo Wang
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 53-60
    |
    Published online on: May 6, 2014
       https://doi.org/10.3892/mmr.2014.2212
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Abstract

Multidrug resistance (MDR) is one of the most important obstacles affecting the efficacy of chemotherapy treatments for numerous types of cancer. In the present study, we have demonstrated the possible function of Twist1 in the chemosensitivity of head and neck squamous cell carcinoma (HNSCC) and have identified that its mechanism maybe associated with MDR1/P-gp regulation. To investigate this, the hypopharyngeal cancer cell line, FaDu, and its MDR cell line induced by taxol, FaDu/T, were employed. Stable transfectants targeted to Twist1 overexpression and Twist1 silencing based on FaDu were also conducted. Morphological observation, flow cytometry, reverse transcription-polymerase chain reaction (RT-PCR), western blotting and laser scanning confocal microscope detection were utilized to detect the associations between Twist1 and the chemosensitivity of FaDu cells. Our results demonstrated that Twist1 and MDR1/P-gp were upregulated in FaDu/T cells in a MDR dose-dependent manner. The anti-apoptotic capabilities of FaDu/T cells were enhanced during MDR progression, with apoptosis-related proteins (Bcl-2, Bax, activated caspase-3 and caspase-9) changing to resist apoptosis. Twist1 overexpression decreased the sensitivity of cells to taxol as revealed by a significant increase in MDR1/P-gp and IC50 (P<0.05). This overexpression also enhanced the resistance to apoptosis, with apoptotic proteins changing to resist cell death, and inhibited Ca2+ release induced by taxol (P<0.05). Detections in Twist1 silencing cells also confirmed this result. This study provided evidence that alterations of Twist1 expression modulates the chemosensitivity of FaDu cells to taxol. Therefore, Twist1 knockdown may be a promising treatment regimen for advanced hypopharyngeal carcinoma patients with MDR.
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Copy and paste a formatted citation
Spandidos Publications style
Lu S, Yu L, Mu Y, Ma J, Tian J, Xu W and Wang H: Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells. Mol Med Rep 10: 53-60, 2014.
APA
Lu, S., Yu, L., Mu, Y., Ma, J., Tian, J., Xu, W., & Wang, H. (2014). Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells. Molecular Medicine Reports, 10, 53-60. https://doi.org/10.3892/mmr.2014.2212
MLA
Lu, S., Yu, L., Mu, Y., Ma, J., Tian, J., Xu, W., Wang, H."Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells". Molecular Medicine Reports 10.1 (2014): 53-60.
Chicago
Lu, S., Yu, L., Mu, Y., Ma, J., Tian, J., Xu, W., Wang, H."Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells". Molecular Medicine Reports 10, no. 1 (2014): 53-60. https://doi.org/10.3892/mmr.2014.2212
Copy and paste a formatted citation
x
Spandidos Publications style
Lu S, Yu L, Mu Y, Ma J, Tian J, Xu W and Wang H: Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells. Mol Med Rep 10: 53-60, 2014.
APA
Lu, S., Yu, L., Mu, Y., Ma, J., Tian, J., Xu, W., & Wang, H. (2014). Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells. Molecular Medicine Reports, 10, 53-60. https://doi.org/10.3892/mmr.2014.2212
MLA
Lu, S., Yu, L., Mu, Y., Ma, J., Tian, J., Xu, W., Wang, H."Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells". Molecular Medicine Reports 10.1 (2014): 53-60.
Chicago
Lu, S., Yu, L., Mu, Y., Ma, J., Tian, J., Xu, W., Wang, H."Role and mechanism of Twist1 in modulating the chemosensitivity of FaDu cells". Molecular Medicine Reports 10, no. 1 (2014): 53-60. https://doi.org/10.3892/mmr.2014.2212
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