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Article

MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase

  • Authors:
    • Xuelan Li
    • Chunfang Li
    • Xin Dong
    • Wenli Gou
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynaecology, The First Affiliated Hospital, Xi'an Jiao Tong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 550-554
    |
    Published online on: May 6, 2014
       https://doi.org/10.3892/mmr.2014.2214
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Abstract

The aim of the present study was to characterize the role of microRNA (miR)-155 in the pathogenesis of severe preeclampsia (PE). A total of 19 severe preeclampsic and 22 normal placentas were collected to measure miR-155 and endothelial nitric oxide synthase (eNOS) expression using quantitative (q)PCR and western blot analysis. The results demonstrated a significant increase in the levels of miR-155 and decreased eNOS expression in the severe preeclampsic placentas, as compared with the normal controls. In order to examine the function of miR-155 in the human placenta, the HTR8/Svneo cell line was transiently transfected with an miR-155 mimic or its inhibitor, anti-miR-155. It was confirmed that miR-155 may suppress the expression of eNOS in HTR-8/SVneo cells. Furthermore, a transwell insert invasion assay demonstrated that miR-155 inhibited cell invasion in trophoblast cells, and the effect was rescued by over expression of eNOS. The present study revealed that miR-155 has a negative regulatory role in the migratory behavior of HTR-8/SVneo cells via modulating eNOS.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Li C, Dong X and Gou W: MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase. Mol Med Rep 10: 550-554, 2014.
APA
Li, X., Li, C., Dong, X., & Gou, W. (2014). MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase. Molecular Medicine Reports, 10, 550-554. https://doi.org/10.3892/mmr.2014.2214
MLA
Li, X., Li, C., Dong, X., Gou, W."MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 10.1 (2014): 550-554.
Chicago
Li, X., Li, C., Dong, X., Gou, W."MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 10, no. 1 (2014): 550-554. https://doi.org/10.3892/mmr.2014.2214
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Li C, Dong X and Gou W: MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase. Mol Med Rep 10: 550-554, 2014.
APA
Li, X., Li, C., Dong, X., & Gou, W. (2014). MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase. Molecular Medicine Reports, 10, 550-554. https://doi.org/10.3892/mmr.2014.2214
MLA
Li, X., Li, C., Dong, X., Gou, W."MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 10.1 (2014): 550-554.
Chicago
Li, X., Li, C., Dong, X., Gou, W."MicroRNA-155 inhibits migration of trophoblast cells and contributes to the pathogenesis of severe preeclampsia by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 10, no. 1 (2014): 550-554. https://doi.org/10.3892/mmr.2014.2214
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