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Article

Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells

  • Authors:
    • In Sik Kim
    • Eun Ju Yang
    • Dong‑Ha Shin
    • Kwang‑Hee Son
    • Ho‑Yong Park
    • Ji‑Sook Lee
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon 301‑746, Republic of Korea, Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Hanny University, Gyeongsan 712‑715, Republic of Korea, Insect Biotech Co., Ltd., Dajeon 305‑811, Republic of Korea, Industrial Bio‑Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305‑806, Republic of Korea, Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan, Chonbuk 570‑750, Republic of Korea
  • Pages: 1025-1029
    |
    Published online on: May 13, 2014
       https://doi.org/10.3892/mmr.2014.2231
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Abstract

Arazyme is a novel extracellular metalloprotease secreted by Aranicola proteolyticus. Endothelial cells are involved in the pathogenesis of a number of inflammatory diseases, induce uncontrolled cell viability and express various inflammatory mediators, including cytokines, chemokines, adhesion molecules and reactive oxygen species (ROS). In the current study, human umbilical vein endothelilal cells (HUVECs) were used to investigate the anti‑inflammatory effects of arazyme following lipopolysaccharide (LPS) stimulation. Apoptosis of HUVECs due to LPS was inhibited by arazyme. In various inflammatory responses induced by LPS, arazyme inhibited the secretion of the monocyte chemoattractant protein‑1 and interleukin‑6, and the expression of vascular cell adhesion molecule‑1 and intercellular adhesion molecule‑1. Arazyme also suppressed ROS production in HUVECs. The action of arazyme was not associated with NF‑κB activity in HUVECs. These results indicate that arazyme has anti‑inflammatory properties in inflamed endothelial cells and may be useful as a therapeutic agent for inflammatory diseases associated with endothelial cells.
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1 

Bersanetti PA, Park HY, Bae KS, Son KH, Shin DH, Hirata IY, Juliano MA, Carmona AK and Juliano L: Characterization of arazyme, an exocellular metalloprotease isolated from Serratia proteamaculans culture medium. Enzyme Microb Technol. 37:574–581. 2005. View Article : Google Scholar

2 

Kwak J, Lee K, Shin DH, Maeng JS, Park DS, Oh HW, Son KH, Bae KS and Park HY: Biochemical and genetic characterization of arazyme, an extracellular metalloprotease produced from Serratia proteamaculans HY-3. J Microbiol Biotechnol. 17:761–768. 2007.PubMed/NCBI

3 

Park JK, Jeong DH, Park HY, Son KH, Shin DH, Do SH, Yang HJ, Yuan DW, Hong IH, Goo MJ, et al: Hepatoprotective effect of arazyme on CCl4-induced acute hepatic injury in SMP30 knock-out mice. Toxicology. 246:132–142. 2008.PubMed/NCBI

4 

Hadi HA, Carr CS and Al Suwaidi J: Endothelial dysfunction: cardiovascular risk factors, therapy, and outcome. Vasc Health Risk Manag. 1:183–198. 2005.PubMed/NCBI

5 

Liu HT, He JL, Li WM, Yang Z, Wang YZ, Yin J, Du YG and Yu C: Geniposide inhibits interleukin-6 and interleukin-8 production in lipopolysaccharide-induced human umbilical vein endothelial cells by blocking p38 and ERK1/2 signaling pathways. Inflamm Res. 59:451–461. 2010. View Article : Google Scholar

6 

Szmitko PE, Wang CH, Weisel RD, Jeffries GA, Anderson TJ and Verma S: Biomarkers of vascular disease linking inflammation to endothelial activation: Part II. Circulation. 108:2041–2048. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Sprague AH and Khalil RA: Inflammatory cytokines in vascular dysfunction and vascular disease. Biochem Pharmacol. 78:539–552. 2009. View Article : Google Scholar : PubMed/NCBI

8 

de Vries IJ, Langeveld-Wildschut EG, van Reijsen FC, Dubois GR, van den Hoek JA, Bihari IC, van Wichen D, de Weger RA, Knol EF, Thepen T and Bruijnzeel-Koomen CA: Adhesion molecule expression on skin endothelia in atopic dermatitis: effects of TNF-alpha and IL-4. J Allergy Clin Immunol. 102:461–468. 1998.PubMed/NCBI

9 

Kim TH and Bae JS: Ecklonia cava extracts inhibit lipopolysaccharide induced inflammatory responses in human endothelial cells. Food Chem Toxicol. 48:1682–1687. 2010. View Article : Google Scholar

10 

Juzyszyn Z, Czerny B, Pawlik A and Droździk M: The effect of Artichoke (Cynara scolymus L.) extract on ROS generatin in HUVEC cells. Phytother Res. 22:1159–1161. 2008.

11 

Lim JH, Woo JS and Shin YW: Cilostazol protects endothelial cells against lipopolysaccharide-induced apoptosis through ERK1/2- and P38 MAPK-dependent pathways. Korean J Intern Med. 24:113–122. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Munshi N, Fernandis AZ, Cherla RP, Park IW and Ganju RK: Lipopolysaccharide-induced apoptosis of endothelial cells and its inhibition by vascular endothelial growth factor. J Immunol. 168:5860–5866. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Marui N, Offermann MK, Swerlick R, Kunsch C, Rosen CA, Ahmad M, Alexander RW and Medford RM: Vascular cell adhesion molecule-1 (VACM-1) gene transcription and expression are regulated through an antioxidant-sensitive mechanism in human vascular endothelial cells. J Clin Invest. 92:1866–1874. 1993. View Article : Google Scholar

14 

Sawa Y, Ueki T, Hata M, Iwasawa K, Tsuruga E, Kojima H, Ishikawa H and Yoshida S: LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 expression in human lymphatic endothelium. J Histochem Cytochem. 56:97–109. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Joyce DE and Grinnell BW: Recombinant human activated protein C attenuates the inflammatory response in endothelium and monocytes by modulating nuclear factor-kappaB. Crit care med. 20(Suppl 5): S288–S293. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Rossi D and Zlotnik A: The biology of chemokines and their receptors. Annu Rev Immunol. 18:217–242. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Yang EJ, Choi E, Ko J, Kim DH, Lee JS and Kim IS: Differential effect of CCL2 on constitutive neutrophil apoptosis between normal and asthmatic subjects. J Cell Physiol. 227:2567–2577. 2012. View Article : Google Scholar

18 

Hirano T: Interleukin 6 and its receptor: ten years later. Int Rev Immunol. 16:249–284. 1998.PubMed/NCBI

19 

Gabay C: Interleukin-6 and chronic inflammation. Arthritis Res Ther. 8(Suppl 2): S32006. View Article : Google Scholar

20 

Bellanti JA: Cytokines and allergic diseases: clinical aspects. Allergy Asthma Proc. 19:337–341. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Viatour P, Merville MP, Bours V and Chariot A: Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation. Trends Biochem Sci. 30:43–52. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Arora P, Ricks TK and Trejo J: Protease-activated receptor signalling, endocytic sorting and dysregulation in cancer. J Cell Sci. 120:921–928. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Hauck G: Proceedings: Vitalmicroscopic investigations of the effects of thrombin, a snake venom enzyme and histamine effect on the mesenteric microvasculature of rabbit and cat. Arzneimittelforschung. 26:12331976.

24 

Wolz RL and Bond JS: Phe5(4-nitro)-bradykinin: a chromogenic substrate for assay and kinetics of the metalloendopeptidase meprin. Anal Biochem. 191:314–320. 1990. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim IS, Yang EJ, Shin DH, Son KH, Park HY and Lee JS: Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells. Mol Med Rep 10: 1025-1029, 2014.
APA
Kim, I.S., Yang, E.J., Shin, D., Son, K., Park, H., & Lee, J. (2014). Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells. Molecular Medicine Reports, 10, 1025-1029. https://doi.org/10.3892/mmr.2014.2231
MLA
Kim, I. S., Yang, E. J., Shin, D., Son, K., Park, H., Lee, J."Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells". Molecular Medicine Reports 10.2 (2014): 1025-1029.
Chicago
Kim, I. S., Yang, E. J., Shin, D., Son, K., Park, H., Lee, J."Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells". Molecular Medicine Reports 10, no. 2 (2014): 1025-1029. https://doi.org/10.3892/mmr.2014.2231
Copy and paste a formatted citation
x
Spandidos Publications style
Kim IS, Yang EJ, Shin DH, Son KH, Park HY and Lee JS: Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells. Mol Med Rep 10: 1025-1029, 2014.
APA
Kim, I.S., Yang, E.J., Shin, D., Son, K., Park, H., & Lee, J. (2014). Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells. Molecular Medicine Reports, 10, 1025-1029. https://doi.org/10.3892/mmr.2014.2231
MLA
Kim, I. S., Yang, E. J., Shin, D., Son, K., Park, H., Lee, J."Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells". Molecular Medicine Reports 10.2 (2014): 1025-1029.
Chicago
Kim, I. S., Yang, E. J., Shin, D., Son, K., Park, H., Lee, J."Effect of arazyme on the lipopolysaccharide‑induced inflammatory response in human endothelial cells". Molecular Medicine Reports 10, no. 2 (2014): 1025-1029. https://doi.org/10.3892/mmr.2014.2231
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