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Article

Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model

  • Authors:
    • Xinling Liang
    • Yuanhan Chen
    • Li Zhang
    • Fen Jiang
    • Wenjian Wang
    • Zhiming Ye
    • Shuangxin Liu
    • Chunping Yu
    • Wei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China, Department of Nephrology, Central Hospital of Hengyang, Hengyang, Hunan 421001, P.R. China
  • Pages: 719-724
    |
    Published online on: May 13, 2014
       https://doi.org/10.3892/mmr.2014.2234
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Abstract

Acute kidney injury (AKI) induced by renal ischemia is a common clinical problem associated with a high morbidity and mortality. The present study investigated whether necroptosis was present in an in vitro renal ischemia model and whether the addition of necrostatin-1 (Nec-1) has a protective effect. In addition, whether autophagy was inhibited following the use of Nec-1 was also examined. When apoptosis was inhibited by z-VAD‑fmk and energy was depleted with antimycin A for 1 h, the morphological abnormalities of human proximal tubular epithelial (HK-2) cells were markedly attenuated, and the cell viability was significantly improved following incubation with Nec-1. LC3-II/I ratios and LC3-II/GAPDH ratios demonstrated a statistically significant decrease in the Nec-1 + tumor necrosis factor (TNF)-α + z-VAD-fmk + antimycin A (1 h) group compared with the control group. In conclusion, the present study suggested that necroptosis was present in HK-2 cells subjected to TNF-α stimulation and energy depletion. Nec-1 inhibits a caspase‑independent necroptotic pathway involving autophagy and may have therapeutic potential to prevent and treat renal ischemic injury.
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Copy and paste a formatted citation
Spandidos Publications style
Liang X, Chen Y, Zhang L, Jiang F, Wang W, Ye Z, Liu S, Yu C and Shi W: Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model. Mol Med Rep 10: 719-724, 2014.
APA
Liang, X., Chen, Y., Zhang, L., Jiang, F., Wang, W., Ye, Z. ... Shi, W. (2014). Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model. Molecular Medicine Reports, 10, 719-724. https://doi.org/10.3892/mmr.2014.2234
MLA
Liang, X., Chen, Y., Zhang, L., Jiang, F., Wang, W., Ye, Z., Liu, S., Yu, C., Shi, W."Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model". Molecular Medicine Reports 10.2 (2014): 719-724.
Chicago
Liang, X., Chen, Y., Zhang, L., Jiang, F., Wang, W., Ye, Z., Liu, S., Yu, C., Shi, W."Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model". Molecular Medicine Reports 10, no. 2 (2014): 719-724. https://doi.org/10.3892/mmr.2014.2234
Copy and paste a formatted citation
x
Spandidos Publications style
Liang X, Chen Y, Zhang L, Jiang F, Wang W, Ye Z, Liu S, Yu C and Shi W: Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model. Mol Med Rep 10: 719-724, 2014.
APA
Liang, X., Chen, Y., Zhang, L., Jiang, F., Wang, W., Ye, Z. ... Shi, W. (2014). Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model. Molecular Medicine Reports, 10, 719-724. https://doi.org/10.3892/mmr.2014.2234
MLA
Liang, X., Chen, Y., Zhang, L., Jiang, F., Wang, W., Ye, Z., Liu, S., Yu, C., Shi, W."Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model". Molecular Medicine Reports 10.2 (2014): 719-724.
Chicago
Liang, X., Chen, Y., Zhang, L., Jiang, F., Wang, W., Ye, Z., Liu, S., Yu, C., Shi, W."Necroptosis, a novel form of caspase-independent cell death, contributes to renal epithelial cell damage in an ATP-depleted renal ischemia model". Molecular Medicine Reports 10, no. 2 (2014): 719-724. https://doi.org/10.3892/mmr.2014.2234
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