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Article

Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function

  • Authors:
    • Chun-Miao Wang
    • Zeng Guo
    • Yang-Jing Xie
    • Yu-Yu Hao
    • Ji‑Min Sun
    • Jian Gu
    • Ai-Ling Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Emergency, The First People's Hospital of Hefei, Hefei, Anhui 230051, P.R. China, Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Cardiology, The First People's Hospital of Hefei, Hefei, Anhui 230051, P.R. China
  • Pages: 792-798
    |
    Published online on: May 13, 2014
       https://doi.org/10.3892/mmr.2014.2236
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Abstract

Inflammatory mediators are released by the myocardium following myocardial ischemia as a response to tissue injury, and contribute to cardiac repair and adaptive responses. Treating mesenchymal stem cells (MSCs) with various inflammatory factors activates a series of biological processes that enhance cell-mediated cardioprotection following myocardial infarction (MI). The present study was designed to examine the effect of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) treatment on vascular cell adhesion molecule-1 (VCAM-1) expression in MSCs, and to identify whether cytokine-treated MSCs improve post-ischemic myocardial function in a rat model. MSCs were stimulated with IL-1β and/or TNF-α for 24 h, the production of vascular cell adhesion molecule-1 (VCAM-1) and the adhesion ability of MSCs were assessed by flow cytometry, adhesion assays, quantitative polymerase chain reaction and western blot analysis. The cardiac function was examined by two-dimensional echocardiography. The results demonstrated that in treated MSCs, the secretion of VCAM-1 and the cell adhesion ability were significantly increased, thus markedly improving cardiac function compared with that of the control group (P<0.01). Of all the groups, the rats stimulated with a combination of IL-1β and TNF-α exhibited the greatest cardiac improvements. However, there was no significant difference between the 10 and 20 ng/ml groups which were stimulated with one of the cytokines alone (P>0.05). In conclusion, stimulating MSCs with IL-1β and TNF-α promoted the expression of VCAM-1 and improved post-ischemic cardiac function recovery. Treating MSCs with two cytokines in combination may be a useful method to maximize the potential of cell-based therapy for MI.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Guo Z, Xie Y, Hao Y, Sun JM, Gu J and Wang A: Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function. Mol Med Rep 10: 792-798, 2014.
APA
Wang, C., Guo, Z., Xie, Y., Hao, Y., Sun, J., Gu, J., & Wang, A. (2014). Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function. Molecular Medicine Reports, 10, 792-798. https://doi.org/10.3892/mmr.2014.2236
MLA
Wang, C., Guo, Z., Xie, Y., Hao, Y., Sun, J., Gu, J., Wang, A."Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function". Molecular Medicine Reports 10.2 (2014): 792-798.
Chicago
Wang, C., Guo, Z., Xie, Y., Hao, Y., Sun, J., Gu, J., Wang, A."Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function". Molecular Medicine Reports 10, no. 2 (2014): 792-798. https://doi.org/10.3892/mmr.2014.2236
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Guo Z, Xie Y, Hao Y, Sun JM, Gu J and Wang A: Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function. Mol Med Rep 10: 792-798, 2014.
APA
Wang, C., Guo, Z., Xie, Y., Hao, Y., Sun, J., Gu, J., & Wang, A. (2014). Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function. Molecular Medicine Reports, 10, 792-798. https://doi.org/10.3892/mmr.2014.2236
MLA
Wang, C., Guo, Z., Xie, Y., Hao, Y., Sun, J., Gu, J., Wang, A."Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function". Molecular Medicine Reports 10.2 (2014): 792-798.
Chicago
Wang, C., Guo, Z., Xie, Y., Hao, Y., Sun, J., Gu, J., Wang, A."Co-treating mesenchymal stem cells with IL‑1β and TNF-α increases VCAM-1 expression and improves post-ischemic myocardial function". Molecular Medicine Reports 10, no. 2 (2014): 792-798. https://doi.org/10.3892/mmr.2014.2236
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