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Article

B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine

  • Authors:
    • Biwen Lin
    • Hua Zhao
    • Jianfeng Fan
    • Fang Xie
    • Wenjuan Wang
    • Xiangyu Ding
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Chinese PLA General Hospital, Beijing 100853, P.R. China
  • Pages: 911-916
    |
    Published online on: May 15, 2014
       https://doi.org/10.3892/mmr.2014.2241
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Abstract

Th1 antigen-specific T cells secrete interferon-γ, which is able to kill antigen-specific cancer cells and is helpful for cancer vaccines. The aim of the present study was to explore whether B16 cell lysates plus polyinosinic-cytidylic acid (poly I:C) can effectively inhibit the progression of melanoma in an animal model. In the present study, C57BL/6 mice were divided into three groups, with each group containing more than six mice. The groups of mice were immunized twice with B16 cell lysates plus poly I:C, B16 cell lysates, or phosphate-buffered saline only, respectively. The in vivo results demonstrated that splenocytes from the mice immunized with B16 cell lysates plus poly I:C contained higher percentages of CD3+CD8+ T lymphocytes and CD3+CD4+ T lymphocytes, which were detected by a fluorescence-activated cell sorter, and produced higher levels of antigen-specific splenocyte proliferation activity, as detected by MTT assay. The splenocytes from the mice immunized with B16 cell lysates in combination with poly I:C produced higher levels of interferon‑γ, as detected by quantitative polymerase chain reaction and ELISA, as well as cytotoxic T lymphocyte activity when stimulated in vitro with B16 lysates. Additionally, subcutaneous immunization of the C57BL/6 mice with B16 cell lysates plus poly I:C conferred greater protection against tumor-forming B16 melanoma cells than that of the mice immunized with injection of B16 cell lysate alone. In conclusion, the cancer vaccine of B16 cell lysates plus poly I:C exerts potently protective effects that polarize responses toward Th1 and elicit antitumor immunity.
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Copy and paste a formatted citation
Spandidos Publications style
Lin B, Zhao H, Fan J, Xie F, Wang W and Ding X: B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine. Mol Med Rep 10: 911-916, 2014.
APA
Lin, B., Zhao, H., Fan, J., Xie, F., Wang, W., & Ding, X. (2014). B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine. Molecular Medicine Reports, 10, 911-916. https://doi.org/10.3892/mmr.2014.2241
MLA
Lin, B., Zhao, H., Fan, J., Xie, F., Wang, W., Ding, X."B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine". Molecular Medicine Reports 10.2 (2014): 911-916.
Chicago
Lin, B., Zhao, H., Fan, J., Xie, F., Wang, W., Ding, X."B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine". Molecular Medicine Reports 10, no. 2 (2014): 911-916. https://doi.org/10.3892/mmr.2014.2241
Copy and paste a formatted citation
x
Spandidos Publications style
Lin B, Zhao H, Fan J, Xie F, Wang W and Ding X: B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine. Mol Med Rep 10: 911-916, 2014.
APA
Lin, B., Zhao, H., Fan, J., Xie, F., Wang, W., & Ding, X. (2014). B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine. Molecular Medicine Reports, 10, 911-916. https://doi.org/10.3892/mmr.2014.2241
MLA
Lin, B., Zhao, H., Fan, J., Xie, F., Wang, W., Ding, X."B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine". Molecular Medicine Reports 10.2 (2014): 911-916.
Chicago
Lin, B., Zhao, H., Fan, J., Xie, F., Wang, W., Ding, X."B16 cell lysates plus polyinosinic-cytidylic acid effectively eradicate melanoma in a mouse model by acting as a prophylactic vaccine". Molecular Medicine Reports 10, no. 2 (2014): 911-916. https://doi.org/10.3892/mmr.2014.2241
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