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Article

Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS

  • Authors:
    • Stephan Albrecht Ender
    • Andrea Dallmer
    • Florian  Lässig
    • Uwe Lendeckel
    • Carmen Wolke
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics and Orthopaedic Surgery, University Medicine Greifswald, Sauerbruchstrasse, Greifswald D-17475, Germany, Institute of Medical Biochemistry and Molecular Biology, University Medicine Greifswald, Sauerbruchstrasse, Greifswald D-17475, Germany
  • Pages: 804-810
    |
    Published online on: May 22, 2014
       https://doi.org/10.3892/mmr.2014.2266
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Abstract

The renin-angiotensin-system (RAS), via its classical angiotensin-converting enzyme (ACE)/angiotensin II/angiotensin II type 1 receptor (AT1R)-axis, is associated with proliferation and metastasis of numerous types of solid tumor. AT1R blockers reduce tumor volume and decrease liver and lung metastasis in murine models of osteosarcoma. Expression and function of the alternative ACE2/Ang(1-7)/Mas axis in osteosarcoma is yet to be studied. In the present study, the basic and interleukin (IL)-1β-stimulated expression of components of this alternative RAS axis were analyzed and the impact of Mas on proliferation and/or migration of U-2 OS and MNNG-HOS osteosarcoma cells was studied. Quantitative polymerase chain reaction revealed that the two cell lines expressed the Ang(1‑7)-generating peptidases ACE2, neutral endopeptidase 24.11 and prolyl-endopeptidase together with the putative receptor for Ang(1-7), Mas. IL-1β provoked an induction of Mas mRNA and protein expression which was associated with a reduction of proliferation and migration. By contrast, small interfering RNA-mediated knockdown of Mas expression led to increased cell proliferation. In conclusion, osteosarcoma cells express a functional active alternative ACE2/Ang(1-7)/Mas axis. The induction and reinforcement of this axis may be beneficial for the treatment of osteosarcoma by reducing growth and preventing cancer metastasis. These effects may be achieved directly by the administration of Mas agonists or, indirectly, via blocking the classical AngII RAS axis via ACE inhibitors or AT1R antagonists.
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Copy and paste a formatted citation
Spandidos Publications style
Ender SA, Dallmer A, Lässig F, Lendeckel U and Wolke C: Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS. Mol Med Rep 10: 804-810, 2014.
APA
Ender, S.A., Dallmer, A., Lässig, F., Lendeckel, U., & Wolke, C. (2014). Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS. Molecular Medicine Reports, 10, 804-810. https://doi.org/10.3892/mmr.2014.2266
MLA
Ender, S. A., Dallmer, A., Lässig, F., Lendeckel, U., Wolke, C."Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS". Molecular Medicine Reports 10.2 (2014): 804-810.
Chicago
Ender, S. A., Dallmer, A., Lässig, F., Lendeckel, U., Wolke, C."Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS". Molecular Medicine Reports 10, no. 2 (2014): 804-810. https://doi.org/10.3892/mmr.2014.2266
Copy and paste a formatted citation
x
Spandidos Publications style
Ender SA, Dallmer A, Lässig F, Lendeckel U and Wolke C: Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS. Mol Med Rep 10: 804-810, 2014.
APA
Ender, S.A., Dallmer, A., Lässig, F., Lendeckel, U., & Wolke, C. (2014). Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS. Molecular Medicine Reports, 10, 804-810. https://doi.org/10.3892/mmr.2014.2266
MLA
Ender, S. A., Dallmer, A., Lässig, F., Lendeckel, U., Wolke, C."Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS". Molecular Medicine Reports 10.2 (2014): 804-810.
Chicago
Ender, S. A., Dallmer, A., Lässig, F., Lendeckel, U., Wolke, C."Expression and function of the ACE2/angiotensin(1-7)/Mas axis in osteosarcoma cell lines U-2 OS and MNNG-HOS". Molecular Medicine Reports 10, no. 2 (2014): 804-810. https://doi.org/10.3892/mmr.2014.2266
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