Bafilomycin A1 inhibits autophagy and induces apoptosis in MG63 osteosarcoma cells

Xie,Zonggang; Xie,Ye; Xu,Youjia; Zhou,Haibin; Xu,Wei; Dong,Qirong

doi:10.3892/mmr.2014.2281

August-2014 Volume 10 Issue 2

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August-2014 Volume 10 Issue 2

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Article

Bafilomycin A1 inhibits autophagy and induces apoptosis in MG63 osteosarcoma cells

Authors:
- Zonggang Xie
- Ye Xie
- Youjia Xu
- Haibin Zhou
- Wei Xu
- Qirong Dong
View Affiliations / Copyright

Affiliations: Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China
Pages: 1103-1107
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Published online on: May 29, 2014

https://doi.org/10.3892/mmr.2014.2281
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Abstract

The purpose of the present study was to investigate the effects of bafilomycin A1 (BafA1) on proliferation, apoptosis and autophagy in MG63 osteosarcoma cells. The growth rate of MG63 cells was determined using a Cell Counting Kit‑8 assay. The mitochondrial membrane potential (Δψ) was measured using a fluorescent probe, JC‑1, and the inhibition of autophagy and apoptosis was monitored by transmission electron microscopy. In addition, the inhibition of autophagy was monitored by western blot analysis of microtubule‑associated protein 1 light chain 3 (LC3), and the ratio of LC3‑II/LC3‑I protein levels was calculated as an indicator of the extent of autophagy. Furthermore, the expression levels of specific proteins associated with autophagy, including p53, Beclin1 and p62, were detected in cultured MG63 cells by western blotting. It was shown that the viability of MG63 cells was inhibited following the use of BafA1, and an induction in the expression levels of the apoptosis‑related protein p53 and the autophagic protein Beclin1 was detected. Furthermore, a collapse in Δψ was observed, together with an induction of apoptotic cell death, following treatment with BafA1. Therefore, following BafA1‑mediated inhibition of autophagy, the inhibition of MG63 cell proliferation and induction of apoptosis were observed.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Bafilomycin A1 inhibits autophagy and induces apoptosis in MG63 osteosarcoma cells

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