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Article

Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy

  • Authors:
    • Ping He
    • Detian Li
    • Beiru Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 638-644
    |
    Published online on: June 6, 2014
       https://doi.org/10.3892/mmr.2014.2304
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Abstract

Ureteral obstruction leads to renal injury and progresses to irreversible renal fibrosis, with tubular cell atrophy and apoptosis. There is conflicting evidence concerning whether losartan (an angiotensin II type I receptor antagonist) mitigates renal interstitial fibrosis and renal tubular epithelial cell apoptosis following unilateral ureteral obstruction (UUO) in animal models. The aim of this study was to investigate the effect and mechanism of losartan on renal tubular cell apoptosis and renal fibrosis in a rat model of UUO. The rats were subjected to UUO by ureteral ligation and were treated with dimethyl sulfoxide (control) or losartan. The controls underwent sham surgery. The renal tissues were collected 3, 5, 7 and 14 days after surgery for measurement of various indicators of renal fibrosis. UUO increased the expression levels of α‑smooth muscle actin and collagen I, and the extent of renal tubular fibrosis and apoptosis in a time‑dependent manner. Losartan treatment partially attenuated these responses. Progression of renal interstitial fibrosis was accompanied by phosphorylation of signal transducer and activator of transcription 3 (STAT3) and altered the expression levels of two apoptosis‑related proteins (Bax and Bcl2). Losartan treatment also partially attenuated these responses. The results indicated that losartan attenuated renal fibrosis and renal tubular cell apoptosis in a rat model of UUO. This effect appeared to be mediated by partial blockage of STAT3 phosphorylation.
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Copy and paste a formatted citation
Spandidos Publications style
He P, Li D and Zhang B: Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy. Mol Med Rep 10: 638-644, 2014.
APA
He, P., Li, D., & Zhang, B. (2014). Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy. Molecular Medicine Reports, 10, 638-644. https://doi.org/10.3892/mmr.2014.2304
MLA
He, P., Li, D., Zhang, B."Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy". Molecular Medicine Reports 10.2 (2014): 638-644.
Chicago
He, P., Li, D., Zhang, B."Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy". Molecular Medicine Reports 10, no. 2 (2014): 638-644. https://doi.org/10.3892/mmr.2014.2304
Copy and paste a formatted citation
x
Spandidos Publications style
He P, Li D and Zhang B: Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy. Mol Med Rep 10: 638-644, 2014.
APA
He, P., Li, D., & Zhang, B. (2014). Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy. Molecular Medicine Reports, 10, 638-644. https://doi.org/10.3892/mmr.2014.2304
MLA
He, P., Li, D., Zhang, B."Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy". Molecular Medicine Reports 10.2 (2014): 638-644.
Chicago
He, P., Li, D., Zhang, B."Losartan attenuates renal interstitial fibrosis and tubular cell apoptosis in a rat model of obstructive nephropathy". Molecular Medicine Reports 10, no. 2 (2014): 638-644. https://doi.org/10.3892/mmr.2014.2304
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