1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells

Hou,Huaxin; Li,Danrong; Jiang,Wei; Liang,Yan; Chen,Donglian; Mo,Yuanyuan

doi:10.3892/mmr.2014.2307

August-2014 Volume 10 Issue 2

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August-2014 Volume 10 Issue 2

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Article

1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells

Authors:
- Huaxin Hou
- Danrong Li
- Wei Jiang
- Yan Liang
- Donglian Chen
- Yuanyuan Mo
View Affiliations / Copyright

Affiliations: Department of Pharmaceutical Analysis, College of Pharmacy, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Cellular Biology, The Medical Scientific Research Centre of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Pages: 965-970
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Published online on: June 10, 2014

https://doi.org/10.3892/mmr.2014.2307
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Abstract

1,8‑dihydroxy‑3‑acetyl‑6‑methyl‑9,10 anthraquinone (DAMA) was synthesized from emodin. In the present study, the activity and the oncosis‑like mechanism of DAMA‑enhanced nasopharyngeal carcinoma (NPC) cell sensitivity to ionizing radiation was examined. The results demonstrated that DAMA has a 1.46‑fold radiosensitisation activity for nasopharyngeal carcinoma CNE 1 cells with a non‑cytotoxic concentration of 10 µg/ml DAMA combined with 2 Gy. Following treatment of DAMA combined with radiation, CNE‑1 cells revealed severe cytoplasmic swelling and vacuolization, swollen mitochondria and dilation of the nuclei without chromatin condensation, yielding a typical morphology of oncosis. Oncosis‑related gene expression of ATP synthase F0 subunit 6, chromatin modifying protein 6 and cyclophilin D mRNA increased significantly in the 8 Gy radiation group and the 2 Gy radiation combined with DAMA group. A significant decrease of ATP synthase protein 8 mRNA was observed and the levels of intracellular ATP were also reduced. In addition, the levels of intracellular Ca2+ were increased. In conclusion, DAMA is a potent radiation sensitizer in nasopharyngeal carcinoma cells and mediates its radiosensitisation via oncosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells

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