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Article

Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus

  • Authors:
    • Qiang Huang
    • Jun Fei
    • Hong-Jun Yu
    • Yuan-Bin Gou
    • Xian-Kai Huang
  • View Affiliations / Copyright

    Affiliations: Trauma Center of Daping Hospital, Third Military Medical University, Chongqing 404100, P.R. China, Rehabilitation Department, Southwest Hospital, Third Military Medical University, Chongqing 404100, P.R. China, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 404100, P.R. China
  • Pages: 825-831
    |
    Published online on: June 10, 2014
       https://doi.org/10.3892/mmr.2014.2309
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Abstract

An understanding of the regulatory mechanisms that drive Staphylococcus aureus biofilm formation may lead to the development of an effective strategy to control the increasing number of refractory clinical infections it causes. The present study examined the effects of the antimicrobial agent human β‑defensin 3 (hBD‑3) and the antibiotics vancomycin and clindamycin on the expression of the S. aureus biofilm formation‑regulating genes, icaA and dltB, during bacterial adhesion and biofilm formation. Transcription (mRNA) levels of dlt and ica genes were measured using quantitative polymerase chain reaction, and slimes of S. aureus biofilm were examined with confocal scanning laser microscopy during S. aureus adhesion and biofilm formation. Although hBD‑3, vancomycin and clindamycin led to significantly attenuated biofilm formation, their treatment‑associated effects on the mRNA expression of dlt and ica were not identical. Vancomycin and clindamycin induced sustained expression of the dlt and ica genes, which may be harnessed to induce biofilm formation. However, hBD‑3 did not have a significant affect on the transcription level of dltB during either bacterial adhesion or biofilm formation. Therefore, the mechanism of hBD‑3 that regulated the suppression of biofilm formation appears to differ from the mechanisms of vancomycin and clindamycin.
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Copy and paste a formatted citation
Spandidos Publications style
Huang Q, Fei J, Yu H, Gou Y and Huang X: Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus. Mol Med Rep 10: 825-831, 2014.
APA
Huang, Q., Fei, J., Yu, H., Gou, Y., & Huang, X. (2014). Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus. Molecular Medicine Reports, 10, 825-831. https://doi.org/10.3892/mmr.2014.2309
MLA
Huang, Q., Fei, J., Yu, H., Gou, Y., Huang, X."Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus". Molecular Medicine Reports 10.2 (2014): 825-831.
Chicago
Huang, Q., Fei, J., Yu, H., Gou, Y., Huang, X."Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus". Molecular Medicine Reports 10, no. 2 (2014): 825-831. https://doi.org/10.3892/mmr.2014.2309
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Q, Fei J, Yu H, Gou Y and Huang X: Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus. Mol Med Rep 10: 825-831, 2014.
APA
Huang, Q., Fei, J., Yu, H., Gou, Y., & Huang, X. (2014). Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus. Molecular Medicine Reports, 10, 825-831. https://doi.org/10.3892/mmr.2014.2309
MLA
Huang, Q., Fei, J., Yu, H., Gou, Y., Huang, X."Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus". Molecular Medicine Reports 10.2 (2014): 825-831.
Chicago
Huang, Q., Fei, J., Yu, H., Gou, Y., Huang, X."Effects of human β-defensin-3 on biofilm formation‑regulating genes dltB and icaA in Staphylococcus aureus". Molecular Medicine Reports 10, no. 2 (2014): 825-831. https://doi.org/10.3892/mmr.2014.2309
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