Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells

  • Authors:
    • Jinghua Luo
    • Yong Yuan
    • Pengyu Chang
    • Dawei Li
    • Zhiqiang Liu
    • Yaqin Qu
  • View Affiliations

  • Published online on: June 10, 2014     https://doi.org/10.3892/mmr.2014.2318
  • Pages: 731-736
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the effects of aloe-emodin (AE) on the radiosensitivity and differentiation of HeLa human cervical cancer cells. Cell proliferation was assessed in the HeLa cervical cancer cell line by a methylthiazolyldiphenyl-tetrazolium bromide assay. Radiosensitivity was determined by a colony‑forming assay. Flow cytometry was used for analysis of cell cycle distribution and apoptosis. The expression of γ-H2AX and cyclin B was assessed by western blotting. Alkaline phosphatase (ALP) activity was measured by an ALP activity kit. It was demonstrated that AE inhibited the proliferation of HeLa cells in a concentration- and time-dependent manner, induced G2/M and S phase cell cycle arrest and enhanced the radiosensitivity of HeLa cells. The combination of AE and radiation induced apoptosis, upregulated cyclin B and γ-H2AX expression and further improved ALP activity compared with treatment with AE or radiation alone. AE enhanced the radiosensitivity of HeLa human cervical cancer cells in vitro, inhibited the proliferation of HeLa cells, induced G2/M phase cell cycle arrest and, in combination with radiation, induced the apoptosis and improved the differentiation of HeLa cells.
View Figures
View References

Related Articles

Journal Cover

August-2014
Volume 10 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Luo J, Yuan Y, Chang P, Li D, Liu Z and Qu Y: Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells. Mol Med Rep 10: 731-736, 2014
APA
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., & Qu, Y. (2014). Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells. Molecular Medicine Reports, 10, 731-736. https://doi.org/10.3892/mmr.2014.2318
MLA
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., Qu, Y."Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells". Molecular Medicine Reports 10.2 (2014): 731-736.
Chicago
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., Qu, Y."Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells". Molecular Medicine Reports 10, no. 2 (2014): 731-736. https://doi.org/10.3892/mmr.2014.2318