Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2014 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2014 Volume 10 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells

  • Authors:
    • Jinghua Luo
    • Yong Yuan
    • Pengyu Chang
    • Dawei Li
    • Zhiqiang Liu
    • Yaqin Qu
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Gynecology, The Tumor Hospital of Jilin, Changchun, Jilin 130021, P.R. China, Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 731-736
    |
    Published online on: June 10, 2014
       https://doi.org/10.3892/mmr.2014.2318
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the effects of aloe-emodin (AE) on the radiosensitivity and differentiation of HeLa human cervical cancer cells. Cell proliferation was assessed in the HeLa cervical cancer cell line by a methylthiazolyldiphenyl-tetrazolium bromide assay. Radiosensitivity was determined by a colony‑forming assay. Flow cytometry was used for analysis of cell cycle distribution and apoptosis. The expression of γ-H2AX and cyclin B was assessed by western blotting. Alkaline phosphatase (ALP) activity was measured by an ALP activity kit. It was demonstrated that AE inhibited the proliferation of HeLa cells in a concentration- and time-dependent manner, induced G2/M and S phase cell cycle arrest and enhanced the radiosensitivity of HeLa cells. The combination of AE and radiation induced apoptosis, upregulated cyclin B and γ-H2AX expression and further improved ALP activity compared with treatment with AE or radiation alone. AE enhanced the radiosensitivity of HeLa human cervical cancer cells in vitro, inhibited the proliferation of HeLa cells, induced G2/M phase cell cycle arrest and, in combination with radiation, induced the apoptosis and improved the differentiation of HeLa cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Shepherd JH: Cervical cancer. Best Pract Res Clin Obstet Gynaecol. 26:293–309. 2012. View Article : Google Scholar

2 

Yashar CM, Spanos WJ, Taylor DD and Gercel-Taylor C: Potentiation of the radiation effect with genistein in cervical cancer cells. Gynecol Oncol. 99:199–205. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Dutta A, Bandyopadhyay S, Mandal C and Chatterjee M: Aloe vera leaf exudate induces a caspase-independent cell death in Leishmania donovani promastigotes. J Med Microbiol. 56:629–636. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Krumbiegel G and Schulz HU: Rhein and aloe-emodin kinetics from senna laxatives in man. Pharmacology. 47:120–124. 1993. View Article : Google Scholar : PubMed/NCBI

5 

Andersen DO, Weber ND, Wood SG, Hughes BG, Murray BK and North JA: In vitro virucidal activity of selected anthraquinones and anthraquinone derivatives. Antiviral Res. 16:185–196. 1991. View Article : Google Scholar : PubMed/NCBI

6 

Arosio B, Gagliano N, Fusaro LM, Parmeggiani L, Tagliabue J, Galetti P, De Castri D, Moscheni C and Annoni G: Aloe-Emodin quinone pretreatment reduces acute liver injury induced by carbon tetrachloride. Pharmacol Toxicol. 87:229–233. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Eshun K and He Q: Aloe vera: a valuable ingredient for the food, pharmaceutical and cosmetic industries - a review. Crit Rev Food Sci Nutr. 44:91–96. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Guo JM, Xiao BX, Liu Q, Zhang S, Liu DH and Gong ZH: Anticancer effect of aloe-emodin on cervical cancer cells involves G2/M arrest and induction of differentiation. Acta Pharmacol Sin. 28:1991–1995. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Xiao B, Guo J, Liu D and Zhang S: Aloe-emodin induces in vitro G2/M arrest and alkaline phosphatase activation in human oral cancer KB cells. Oral Oncol. 43:905–910. 2007. View Article : Google Scholar

10 

Lin JG, Chen GW, Li TM, Chouh ST, Tan TW and Chung JG: Aloe-emodin induces apoptosis in T24 human bladder cancer cells through the p53 dependent apoptotic pathway. J Urol. 175:343–347. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Chen HC, Hsieh WT, Chang WC and Chung JG: Aloe-emodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells. Food Chem Toxicol. 42:1251–1257. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Suboj P, Babykutty S, Srinivas P and Gopala S: Aloe emodin induces G2/M cell cycle arrest and apoptosis via activation of caspase-6 in human colon cancer cells. Pharmacology. 89:91–98. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Lin ML, Lu YC, Chung JG, Li YC, Wang SG, NGSH, Wu CY, Su HL and Chen SS: Aloe-emodin induces apoptosis of human nasopharyngeal carcinoma cells via caspase-8-mediated activation of the mitochondrial death pathway. Cancer Lett. 291:46–58. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Guo J, Xiao B, Zhang S, Liu D, Liao Y and Sun Q: Growth inhibitory effects of gastric cancer cells with an increase in S phase and alkaline phosphatese activity repression by aloe-emodin. Cancer Biol Ther. 6:85–88. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Chiu TH, Lai WW, Hsia TC, et al: Aloe-emodin induces cell death through S-phase arrest and caspase-dependent pathways in human tongue squamous cancer SCC-4 Cells. Anticancer Res. 29:4503–4511. 2009.PubMed/NCBI

16 

Pawlik TM and Keyomarsi K: Role of the cell cycle in mediating sensitivity to radiotherapy. Int J Radiat Oncol Biol Phys. 59:928–942. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Terasima T and Tolmach LJ: X-ray sensitivity and DNA synthesis in synchronous populations of HeLa cells. Science. 140:490–492. 1963. View Article : Google Scholar : PubMed/NCBI

18 

Sinclair WK and Morton RA: X-ray sensitivity during the cell generation cycle of cultured Chinese hamster cells. Radiat Res. 29:450–474. 1966. View Article : Google Scholar : PubMed/NCBI

19 

Sinclair WK: Cyclic x-ray responses in mammalian cells in vitro. Radiat Res. 33:620–643. 1968. View Article : Google Scholar : PubMed/NCBI

20 

Chu HL, Mao H, Feng W, Liu JW and Geng Y: Effects of sulfated polysaccharide from Masson pine (Pinus massoniana) pollen on the proliferation and cell cycle of HepG2 cells. Int J Biol Macromol. 55:104–108. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Westendorf JM, Swenson KI and Ruderman JV: The role of cyclin B in meiosis I. J Cell Biol. 108:1431–1444. 1989. View Article : Google Scholar : PubMed/NCBI

22 

West MH and Bonner WM: Histone 2A, a heteromorphous family of eight protein species. Biochemistry. 19:3238–3245. 1980. View Article : Google Scholar : PubMed/NCBI

23 

Bonner WM, Redon CE, Dickey JS, Nakamura AJ, Sedelnikova OA, Solier S and Pommier Y: Gamma H2AX and cancer. Nat Rev Cancer. 8:957–967. 2008.PubMed/NCBI

24 

Rogakou EP, Boon C, Redon C and Bonner WM: Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol. 146:905–916. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Sedelnikova OA, Pilch DR, Redon C and Bonner WM: Histone H2AX in DNA damage and repair. Cancer Biol Ther. 2:233–235. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Celeste A, Fernandez-Capetillo O, Kruhlak MJ, Pilch DR, Staudt DW, Lee A, Bonner RF, Bonner WM and Nussenzweig A: Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks. Nat Cell Biol. 5:675–679. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Ward IM, Minn K, Jorda KG and Chen J: Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX. J Biol Chem. 278:19579–19582. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Baritaki S, Sifakis S, Huerta-Yepez S, et al: Overexpression of VEGF and TGF-beta1 mRNA in Pap smears correlates with progression of cervical intraepithelial neoplasia to cancer: implication of YY1 in cervical tumorigenesis and HPV infection. Int J Oncol. 31:69–79. 2007.

29 

Arvanitis DA and Spandidos DA: Deregulation of the G1/S phase transition in cancer and squamous intraepithelial lesions of the uterine cervix: a case control study. Oncol Rep. 20:751–760. 2008.PubMed/NCBI

30 

Rudel T and Bokoch GM: Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2. Science. 276:1571–1574. 1997. View Article : Google Scholar : PubMed/NCBI

31 

Xiao B, Guo J, Lou Y, Meng D, Zhao W, Zhang L, Yan C and Wang D: Inhibition of growth and increase of alkaline phosphatase activity in cultured human oral cancer cells by all-trans retinoic acid. Int J Oral Maxillofac Surg. 35:643–648. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Dabare AA, Nouri AM, Cannell H, Moss T, Nigam AK and Oliver RT: Profile of placental alkaline phosphatase expression in human malignancies: effect of tumour cell activation on alkaline phosphatase expression. Urol Int. 63:168–174. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Leng B, Liu XD and Chen QX: Inhibitory effects of anticancer peptide from Mercenaria on the BGC-823 cells and several enzymes. FEBS Lett. 579:1187–1190. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Pecere T, Gazzola MV, Mucignat C, Parolin C, Vecchia FD, Cavaggioni A, Basso G, Diaspro A, Salvato B, Carli M and Palù G: Aloe-emodin is a new type of anticancer agent with selective activity against neuroectodermal tumors. Cancer Res. 60:2800–2804. 2000.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Luo J, Yuan Y, Chang P, Li D, Liu Z and Qu Y: Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells. Mol Med Rep 10: 731-736, 2014.
APA
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., & Qu, Y. (2014). Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells. Molecular Medicine Reports, 10, 731-736. https://doi.org/10.3892/mmr.2014.2318
MLA
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., Qu, Y."Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells". Molecular Medicine Reports 10.2 (2014): 731-736.
Chicago
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., Qu, Y."Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells". Molecular Medicine Reports 10, no. 2 (2014): 731-736. https://doi.org/10.3892/mmr.2014.2318
Copy and paste a formatted citation
x
Spandidos Publications style
Luo J, Yuan Y, Chang P, Li D, Liu Z and Qu Y: Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells. Mol Med Rep 10: 731-736, 2014.
APA
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., & Qu, Y. (2014). Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells. Molecular Medicine Reports, 10, 731-736. https://doi.org/10.3892/mmr.2014.2318
MLA
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., Qu, Y."Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells". Molecular Medicine Reports 10.2 (2014): 731-736.
Chicago
Luo, J., Yuan, Y., Chang, P., Li, D., Liu, Z., Qu, Y."Combination of aloe-emodin with radiation enhances radiation effects and improves differentiation in human cervical cancer cells". Molecular Medicine Reports 10, no. 2 (2014): 731-736. https://doi.org/10.3892/mmr.2014.2318
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team