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Article

Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway

  • Authors:
    • Cong Liu
    • Lin Yang
    • Hongxing Dang
    • Fang Fang
    • Feng Xu
  • View Affiliations / Copyright

    Affiliations: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Pediatric Intensive Care Unit, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China
  • Pages: 1604-1608
    |
    Published online on: June 16, 2014
       https://doi.org/10.3892/mmr.2014.2330
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Abstract

Oxidative stress injury and cell death in alveolar epithelial cells may lead to abnormal repair, further resulting in acute and chronic pulmonary diseases. Substance P (SP) has multiple biological activities. The Sonic hedgehog (SHH) pathway is important in lung development and decreasing epithelial injury. To investigate the effects of SP on alveolar epithelial type II cells (AEC IIs), AEC IIs were exposed to 95% oxygen and the SHH signaling pathway was examined. Primary AEC IIs were isolated and purified from premature rats. The cells were divided into four groups: The air (21% oxygen) group, hyperoxia (95% oxygen) group, hyperoxia + SP group and hyperoxia + SP + L703.606 group. The activity of AEC IIs was examined using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. The apoptotic rate of AEC IIs was analyzed by flow cytometry. The oxidative damage was evaluated by flow cytometry and reactive oxygen species (ROS) were detected using a 2',7'‑dichlorodihydrofluorescein diacetate probe. Quantitative polymerase chain reaction and western blotting were used to detect the mRNA and protein expression of the SHH signaling molecule Smoothened (SMO). The results demonstrated that exposure to 95% oxygen for 24 h significantly increased the level of ROS, contributed to apoptosis and markedly decreased the proliferation of AEC IIs. Compared with hyperoxia exposure, SP treatment decreased the level of ROS, reduced AEC II apoptosis and improved the cell survival sequentially. SMO was found to be expressed in AEC IIs and its expression increased when the cells were in hyperoxic conditions. These effects were enhanced by treatment with SP, which was able to significantly increase the expression of SMO. The aforementioned protective effect was weakened following treatment with L703.606. These findings suggested that SP was a protective regulatory factor that was able to decrease the hyperoxia‑induced cell injury and death, and improve the survival of AEC IIs exposed to hyperoxia, which may be associated with the activation of the SHH signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Yang L, Dang H, Fang F and Xu F: Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway. Mol Med Rep 10: 1604-1608, 2014.
APA
Liu, C., Yang, L., Dang, H., Fang, F., & Xu, F. (2014). Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway. Molecular Medicine Reports, 10, 1604-1608. https://doi.org/10.3892/mmr.2014.2330
MLA
Liu, C., Yang, L., Dang, H., Fang, F., Xu, F."Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway". Molecular Medicine Reports 10.3 (2014): 1604-1608.
Chicago
Liu, C., Yang, L., Dang, H., Fang, F., Xu, F."Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway". Molecular Medicine Reports 10, no. 3 (2014): 1604-1608. https://doi.org/10.3892/mmr.2014.2330
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Yang L, Dang H, Fang F and Xu F: Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway. Mol Med Rep 10: 1604-1608, 2014.
APA
Liu, C., Yang, L., Dang, H., Fang, F., & Xu, F. (2014). Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway. Molecular Medicine Reports, 10, 1604-1608. https://doi.org/10.3892/mmr.2014.2330
MLA
Liu, C., Yang, L., Dang, H., Fang, F., Xu, F."Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway". Molecular Medicine Reports 10.3 (2014): 1604-1608.
Chicago
Liu, C., Yang, L., Dang, H., Fang, F., Xu, F."Effect of Substance P on type II alveolar epithelial cells exposed to hyperoxia and its regulation of the Sonic hedgehog signaling pathway". Molecular Medicine Reports 10, no. 3 (2014): 1604-1608. https://doi.org/10.3892/mmr.2014.2330
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