Diabetes mellitus potentiates diffuse large B‑cell lymphoma via high levels of CCL5

Zhang,Jingcheng; Luo,Jia; Liu,Fanrong; Wu,Dongmei; Zhong,Qingling; Zeng,Liangtao; Wu,Ziqing; Zhao,Tong; Wu,Liqing; Hao,Hua

doi:10.3892/mmr.2014.2341

September-2014 Volume 10 Issue 3

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September-2014 Volume 10 Issue 3

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Article

Diabetes mellitus potentiates diffuse large B‑cell lymphoma via high levels of CCL5

Authors:
- Jingcheng Zhang
- Jia Luo
- Fanrong Liu
- Dongmei Wu
- Qingling Zhong
- Liangtao Zeng
- Ziqing Wu
- Tong Zhao
- Liqing Wu
- Hua Hao
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Affiliations: Department of Pathology, Second Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330006, P.R. China , College of Nursing, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Pages: 1231-1236
|
Published online on: June 17, 2014

https://doi.org/10.3892/mmr.2014.2341
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Abstract

There is much evidence suggesting that CCL5 is one of the chemoattractant cytokines involved in diabetes mellitus (DM) with diffuse large B‑cell lymphoma (DLBCL). However, the pathological impact is unclear. In the current study, in order to improve understanding regarding the role of CCL5 in DM with DLBCL, the expression levels of CCL5 mRNA were examined in normal B cells, human DLBCL cell lines (Ly1, Ly8 and Ly10) and a mouse DLBCL cell line (A20), as well as those in cells cultured with either 5 or 30 mmol/l glucose. A20‑CCL5+ (CCL5 overexpression) and A20‑CCL5‑ (CCL5 knockdown) subclones were obtained through cell transduction with a lentiviral vector, and were subcutaneously injected into BALB/c DM mice and normal mice. Tumor growth was observed by calculating the tumor volume. The results demonstrated that CCL5 mRNA levels in DLBCL cells were significantly higher than those in the normal cells (P<0.05); and levels in DLBCL cells in 30 mmol/l Glu were significantly higher than in those of DLBCL cells in 5 mmol/l Glu (P<0.05). A20‑CCL5+ cells led to tumor formation in DM mice compared with A20 and A20‑CCL5‑ cells. These results indicate that high levels of CCL5 expression may accelerate DLBCL formation in DM.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Diabetes mellitus potentiates diffuse large B‑cell lymphoma via high levels of CCL5

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